Mouse ASGR1/ASGPR1 Antibody

Catalog #: MAB27551 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB27551-100
MAB27551-SP

Mouse ASGR1/ASGPR1 ELISA Standard Curve.

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All ASGR1/ASGPR1 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse ASGR1/ASGPR1 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse ASGPR1 in direct ELISAs.

Source

Monoclonal Rat IgG_2A Clone # 352802.113

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse ASGPR1
Ser60-Asn284
Accession # NP_033844

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA capture antibody when paired with Rat Anti-Mouse ASGR1/ASGPR1 Monoclonal Antibody (Catalog # MAB27552).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA

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Mouse ASGR1/ASGPR1 ELISA Standard Curve. Recombinant Mouse ASGR1/ASGPR1 protein was serially diluted 2-fold and captured by Rat Anti-Mouse ASGR1/ASGPR1 Monoclonal Antibody (Catalog # MAB27551) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Mouse ASGR1/ASGPR1 Monoclonal Antibody (Catalog # MAB27552) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ASGR1/ASGPR1

The mouse asialoglycoprotein receptor (ASGP-R) is an endocytic recycling receptor that belongs to the long-form subfamily of the C-type/Ca⁺⁺-dependent lectin family (1‑3). It is a complex of two non‑covalently linked subunits, a major 42 kDa glycoprotein (ASGPR1), and a minor 51 kDa glycoprotein (ASGR2). The major mouse ASGP‑R subunit, ASGPR1, is synthesized as a 284 amino acid (aa) type II transmembrane (TM) protein that contains a 39 aa cytoplasmic region, a 21 aa TM segment, and a 224 aa extracellular domain (ECD) (4-6). The ECD contains two important structural regions. The first is a stalk region of 56 aa (aa’s 59-117) that contributes to non-covalent oligomerization. The second is a 118 aa, carbohydrate-binding, Ca⁺⁺-dependent C-type lectin domain (aa’s 160-277) that is unusually stabilized by three Ca⁺⁺ ions (3, 5). There are two potential alternate splice forms for ASGPR1. Both are TM and show a deletion of the C-type lectin domain. One is 113 aa in length and shows a deletion of aa’s 114-284 (7). The second is 132 aa in length and shows a deletion of aa’s 118-146 and aa’s 162-284 (8). Mouse ASGPR1 ECD is 89% and 79% aa identical to the ASGPR1 ECD in rat and human, respectively. The minor mouse ASGP-R subunit, ASGR2, is also a C-type lectin that shares the same structural organization as ASGR-1. It is 301 aa in length and has two 45 kDa and 51 kDa differentially-glycosylated isoforms (4, 6, 9). The ECD of ASGR2 is 50% aa identical to the ECD of ASGPR1. Although ASGPR1 and 2 can be expressed individually, a fully functional and stable ASGP-R requires simultaneous expression of both subunits (10-12). The stoichiometry of a functional ASGP-R is suggested to be either a 2:2, 3:1 or 3:2 ratio of ASGPR1:ASGR2 (13, 14). ASGPR1 is reported to bind Gal (nonreducing), GalNAc, and sialic acid alpha 2,6GalNAc (3, 15, 16). This is generally in the context of triantennary or tetraantennary configurations (2).

References

Stockert, R. J. (1995) Physiol. Rev. 75:591.
Weigel, P.H. and J.H.N. Yik (2002) Biochim. Biophys. Acta 1572:341.
Meier, M. et al. (2000) J. Mol. Biol. 300:857.
Takezawa, R. et al. (1993) Biochim. Biophys. Acta 1172 :220.
Monroe, R.S. and B.E. Huber (1994) Gene 148:237.
Sanford, J.P. et al. (1988) DNA 7:721.
Heath, P. (2005) GenBank Accession #:Q5NCV2
Heath, P. (2005) GenBank Accession #:Q5NCV1.
Sanford, J.P. and D. Doyle (1990) Biochim. Biophys. Acta 1087:259.
Braun, J.R. et al. (1996) J. Biol. Chem. 271:21160.
Ishibashi, S. et al. (1994) J. Biol. Chem. 269:27803.
Tozawa, R. et al. (2001) J. Biol. Chem. 276:12624.
Bider, M.D. et al. (1996) J. Biol. Chem. 271:31996.
Lodish, H. (1991) Trends Biochem. Sci. 16:374.
Westerlind, U. et al. (2004) Glyconj. J. 21:227.
Park, E.I. et al. (2005) Proc. Natl. Acad. Sci. USA 102:17125.

Long Name

Asialoglycoprotein Receptor 1

Entrez Gene IDs

432 (Human); 11889 (Mouse); 24210 (Rat)

Alternate Names

ASGPR 1; ASGP-R 1; ASGPR; ASGPR1; ASGR1; asialoglycoprotein receptor 1; CLEC4H1; CLEC4H1ASGPR1; C-type lectin domain family 4 member H1; Hepatic lectin H1; HL-1; MHL1; RHL1