Mouse Autoimmune Regulator/AIRE Antibody

Catalog #: AF6184 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6184
AF6184-SP

Detection of Mouse Autoimmune Regulator/AIRE by Western Blot.

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Mouse Autoimmune Regulator/AIRE Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Autoimmune Regulator/AIRE in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse Autoimmune Regulator/AIRE
Ser476-Ser552
Accession # Q9Z0E3

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse Autoimmune Regulator/AIRE by Western Blot. Western blot shows lysates of mouse thymus tissue. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Mouse Autoimmune Regulator/AIRE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6184) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Autoimmune Regulator/AIRE at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Autoimmune Regulator/AIRE

AIRE (Autoimmune regulator; also APECED) is a 58-60 kDa transcriptional regulator that exhibits tight regulation of its expression. It is widely expressed in lymphoid organs, but is most pronounced in thymic medullary epithelium, where it induces the expression of organ specific antigens that are used to sequester and delete autoreactive lymphocytes. Mouse AIRE is 552 amino acids (aa) in length. It contains multiple domains, including an HSR domain with two LXXLL motifs (aa 1-106), an NLS (aa 114-134), one SAND domain (aa 182-282) and two PHD zinc-finger regions (aa 298-345; 434-475). AIRE will homodimerize and tetramerize. There are multiple potential isoforms that involve various combinations of deletions of aa 265-268, 368-426 and Lys296, plus a 42 aa substitution for aa 368-552. Over aa 476-552, mouse AIRE shares 91% and 65% aa identity with rat and human AIRE, respectively.

Entrez Gene IDs

326 (Human); 11634 (Mouse); 294328 (Rat)

Alternate Names

AIRE; APECED protein; APECED; APECEDAPSI; APS1; APSI; Autoimmune polyendocrinopathy candidiasis ectodermal dystrophy protein; autoimmune regulator (APECED protein)10APS1AIRE1; autoimmune regulator (autoimmune polyendocrinopathy candidiasis ectodermaldystrophy); Autoimmune Regulator; PGA1