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Mouse BAFF/BLyS/TNFSF13B Quantikine ELISA Kit

Catalog #: MBLYS0
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MBLYS0
Mouse BAFF/BLyS/TNFSF13B ELISA Standard Curve
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Citations (30)
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Summary Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Mouse BAFF/BLyS/TNFSF13B Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (40 uL), Serum (17 uL), EDTA Plasma (17 uL), Heparin Plasma (17 uL)
Sensitivity
7.8 pg/mL
Assay Range
46.9 - 3,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant mouse BAFF/BLyS
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.

Product Summary

The Quantikine Mouse BAFF/BLyS Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse BAFF/BLyS in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant mouse BAFF/BLyS and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse BAFF/BLyS showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse BAFF/BLyS.

Recovery

The recovery of mouse BAFF/BLyS spiked to three levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=6) 103 90-108
EDTA Plasma (n=6) 96 85-103
Heparin Plasma (n=6) 94 85-100
Serum (n=6) 98 86-115

Linearity

To assess the linearity of the assay, samples containing mouse BAFF/BLyS in each matrix were diluted with Calibrator Diluent and assayed.
Mouse BAFF/BLyS/TNFSF13B ELISA Linearity

Scientific Data

N/A Mouse BAFF/BLyS/TNFSF13B ELISA Standard Curve View Larger

Mouse BAFF/BLyS/TNFSF13B ELISA Standard Curve

ELISA Detection of Mouse BAFF/BLyS/TNFSF13B by ELISA View Larger

Detection of Mouse BAFF/BLyS/TNFSF13B by ELISA OTULIN Deficiency in Myeloid Cells Leads to Neutrophil Infiltration, Multi-organ Inflammation, Hyper-Immunoglobulinemia, and Autoimmunity(A and B) Micrographs of H&E and Masson’s trichome stained sections of livers and spleens from 4- to 9-months-old LysMCre-Otulinflox mice reveal (A) infiltration in and fibrosis (right, arrowheads) of liver parenchyma and (B) distorted spleen architecture with germinal center activation (left, arrowheads) and fibrosis (right, arrowheads) in LysMCre-OtulinLacZ/flox mice. Scale bars, 200 μm. Micrographs are representative of five mice of each genotype from two independent experiments.(C and D) Flow cytometry analysis of total cellular infiltrate (CD45.1+ and CD45.2+) in peritoneal lavage (PL), spleen, liver, and kidney of LysMCre-Otulinflox mice presented as (C) representative dot plots of neutrophils with percent of cells in gate indicated and (D) total cell number of neutrophils, macrophages, and CD8+ T cells.(E) Concentrations of immunoglobulins in serum from 4- to 9-months-old LysMCre-Otulinflox mice.(F) ELISA analysis of serum autoantibody reactivity to ENA, dsDNA, and Smith antigen from 3- to 9-months-old LysMCre-Otulinflox mice.(G) ELISA analysis of serum B cell activating factor (BAFF) concentration from 3- to 9-months-old LysMCre-Otulinflox mice.(E–G) Data are mean of two technical replicas.(D–F) Data are presented as mean ± SEM, and n represents number of mice.See also Figure S5. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27523608), licensed under a CC-BY license. Not internally tested by R&D Systems.

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: BAFF/BLyS/TNFSF13B

BAFF (B-cell activating factor), also known as BLyS, TALL-1, THANK, and TNFSF13B, is a TNF superfamily ligand that plays a critical role in the development and survival of B lineage cells. It can be expressed as a homotrimer or as a heteromer in association with the related protein APRIL. It is produced by many hematopoietic cell types, and a soluble form can be released by activated neutrophils. Both BAFF and APRIL signal through the receptors BCMA and TACI, and BAFF additionally signals through BAFF R. Mice that overexpress BAFF exhibit elevated B cell numbers, increased formation and size of germinal centers, and symptoms of autoimmunity. In addition, BAFF costimulates T cell activation, promotes a Th1 biased immune response, and promotes the expansion of Treg cells.

Long Name:
B cell Activating Factor
Entrez Gene IDs:
10673 (Human); 24099 (Mouse); 498666 (Rat); 102123214 (Cynomolgus Monkey)
Alternate Names:
ApoL related ligand TALL-1; B lymphocyte stimulator; BAFF; BAFFB-cell activating factor; B-cell-activating factor; BLyS; BLYSB-lymphocyte stimulator; CD257 antigen; CD257; Dendritic cell-derived TNF-like molecule; DTL; TALL1; TALL-1delta BAFF; TALL1Delta4 BAFF; THANK; TNF- and APOL-related leukocyte expressed ligand 1; TNF and ApoL-related leukocyte expressed ligand 1; TNF homolog that activates apoptosis; TNFSF13B; TNFSF20; tumor necrosis factor (ligand) superfamily, member 13b; tumor necrosis factor (ligand) superfamily, member 20; tumor necrosis factor ligand superfamily member 13B; tumor necrosis factor superfamily, member 13b; tumor necrosis factor-like protein ZTNF4; ZTNF4
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 80 µL Assay Diluent
  3.   Add 80 µL of Assay Diluent to each well.
    4. 40 µL Standard, Control, or Sample
  4.   Add 40 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  5.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.
    6. 120 µL Conjugate
  6.   Add 120 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  7.   Aspirate and wash 5 times.
    8. 120 µL Substrate Solution
  8.   Add 120 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
    9. 120 µL Stop Solution
  9.   Add 120 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Mouse BAFF/BLyS/TNFSF13B Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

30 Citations: Showing 1 - 10
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  1. Berberine might block colorectal carcinogenesis by inhibiting the regulation of B-cell function by Veillonella parvula
    Authors: Qian, Y;Kang, Z;Zhao, L;Chen, H;Zhou, C;Gao, Q;Wang, Z;Liu, Q;Cui, Y;Li, X;Chen, Y;Zou, T;Fang, J;
    Chinese medical journal
    Species: Mouse
    Sample Types: Serum
  2. A dominant function of LynB kinase in preventing autoimmunity
    Authors: BF Brian, ML Sauer, JT Greene, SE Senevirath, AJ Lindstedt, OL Funk, BL Ruis, LA Ramirez, JL Auger, WL Swanson, MG Nunez, BS Moriarity, CA Lowell, BA Binstadt, TS Freedman
    Science Advances, 2022-04-22;8(16):eabj5227.
    Species: Mouse
    Sample Types: Serum
  3. Homeostatic apoptosis prevents competition-induced atrophy in follicular B cells
    Authors: S Chappaz, K McArthur, L Kealy, CW Law, M Tailler, RM Lane, A Lieschke, ME Ritchie, KL Good-Jacob, A Strasser, BT Kile
    Cell Reports, 2021-07-20;36(3):109430.
    Species: Mouse
    Sample Types: Serum
  4. Mechanism of Graft Damage Caused by NTPDase1-activated Macrophages in Acute Antibody-Mediated Rejection
    Authors: X Peng, Z Yong, W Xiaoyan, C Yuanshan, W Guangzhu, L Xuehuan
    Transplant. Proc., 2020-08-06;0(0):.
    Species: Mouse
    Sample Types: Serum
  5. Transplantation tolerance modifies donor-specific B cell fate to suppress de novo alloreactive B cells
    Authors: SH Khiew, D Jain, J Chen, J Yang, D Yin, JS Young, A Dent, R Sciammas, ML Alegre, AS Chong
    J. Clin. Invest., 2020-07-01;0(0):.
    Species: Mouse
    Sample Types: BAFF
  6. Low-dose 17&alpha-ethinyl estradiol (EE) exposure exacerbates lupus renal disease and modulates immune responses to TLR7/9 agonists in genetically autoimmune-prone mice
    Authors: MR Edwards, R Dai, B Heid, C Cowan, SR Werre, T Cecere, SA Ahmed
    Sci Rep, 2020-03-23;10(1):5210.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  7. Depletion of B cell-activating factor attenuates hepatic fat accumulation in a murine model of nonalcoholic fatty liver disease
    Authors: Y Nakamura, M Abe, K Kawasaki, T Miyake, T Watanabe, O Yoshida, M Hirooka, B Matsuura, Y Hiasa
    Sci Rep, 2019-01-30;9(1):977.
    Species: Mouse
    Sample Types: Serum
  8. Testosterone is an endogenous regulator of BAFF and splenic B cell number
    Authors: AS Wilhelmson, M Lantero Ro, A Stubelius, P Fogelstran, I Johansson, MB Buechler, S Lianoglou, VN Kapoor, ME Johansson, JB Fagman, A Duhlin, P Tripathi, A Camponesch, BT Porse, AG Rolink, H Nissbrandt, SJ Turley, H Carlsten, IL Mårtensson, MCI Karlsson, Å Tivesten
    Nat Commun, 2018-05-25;9(1):2067.
    Species: Mouse
    Sample Types: Serum
  9. Expression of the B cell differentiation factor BAFF and chemokine CXCL13 in a murine model of Respiratory Syncytial Virus infection
    Authors: W Alturaiki, AJ McFarlane, K Rose, R Corkhill, PS McNamara, J Schwarze, BF Flanagan
    Cytokine, 2018-02-01;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  10. BAFF and CD4T cells are major survival factors for long-lived splenic plasma cells in a B-cell-depletion context
    Authors: LH Thai, S Le Gallou, A Robbins, E Crickx, T Fadeev, Z Zhou, N Cagnard, J Mégret, C Bole, JC Weill, CA Reynaud, M Mahévas
    Blood, 2018-01-29;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  11. Irgm1 coordinately regulates autoimmunity and host defense at select mucosal surfaces
    Authors: KM Azzam, JH Madenspach, DW Cain, L Lai, KM Gowdy, P Rai, K Janardhan, N Clayton, W Cunningham, H Jensen, PS Patel, JF Kearney, GA Taylor, MB Fessler
    JCI Insight, 2017-08-17;2(16):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  12. B Cell Homeostasis and Functional Properties Are Altered in an Hypochlorous Acid-Induced Murine Model of Systemic Sclerosis
    Authors: S Sanges, M Jendoubi, N Kavian, C Hauspie, S Speca, JC Crave, T Guerrier, G Lefèvre, V Sobanski, A Savina, E Hachulla, PY Hatron, M Labalette, F Batteux, S Dubucquoi, D Launay
    Front Immunol, 2017-02-07;8(0):53.
    Species: Mouse
    Sample Types: Serum
  13. Elevated GDF-15 contributes to pulmonary inflammation upon cigarette smoke exposure
    Authors: FM Verhamme, LJ Seys, EG De Smet, S Provoost, W Janssens, D Elewaut, GF Joos, GG Brusselle, KR Bracke
    Mucosal Immunol, 2017-02-01;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  14. The Deubiquitinase OTULIN Is an Essential Negative Regulator of Inflammation and Autoimmunity
    Cell, 2016-08-11;0(0):.
    Species: Mouse
    Sample Types: Serum
  15. BANK1 Regulates IgG Production in a Lupus Model by Controlling TLR7-Dependent STAT1 Activation
    PLoS ONE, 2016-05-26;11(5):e0156302.
    Species: Mouse
    Sample Types: Serum
  16. MRL Strains Have a BAFFR Mutation without Functional Consequence
    PLoS ONE, 2016-05-05;11(5):e0154518.
    Species: Mouse
    Sample Types: Serum
  17. Nfkb1 activation by the E26 transformation-specific transcription factors PU.1 and Spi-B promotes Toll-like receptor-mediated splenic B cell proliferation.
    Authors: Li S, Abbas A, Solomon L, Groux G, DeKoter R
    Mol Cell Biol, 2015-03-02;35(9):1619-32.
    Species: Mouse
    Sample Types: Serum
  18. The B lymphocyte differentiation factor (BAFF) is expressed in the airways of children with CF and in lungs of mice infected with Pseudomonas aeruginosa.
    Authors: Neill, Daniel R, Saint, Gemma L, Bricio-Moreno, Laura, Fothergill, Joanne L, Southern, Kevin W, Winstanley, Craig, Christmas, Stephen, Slupsky, Joseph R, McNamara, Paul S, Kadioglu, Aras, Flanagan, Brian F
    PLoS ONE, 2014-05-21;9(5):e95892.
    Species: Mouse
    Sample Types: Tissue Homogenates
  19. Impaired B cell receptor signaling is responsible for reduced TACI expression and function in X-linked immunodeficient mice.
    Authors: Uslu K, Coleman A, Allman W, Katsenelson N, Bram R, Alugupalli K, Akkoyunlu M
    J Immunol, 2014-03-19;192(8):3582-95.
    Species: Mouse, Transgenic Mouse
    Sample Types: Serum
  20. Role of Lactobacillus pentosus Strain b240 and the Toll-like receptor 2 axis in Peyer's patch dendritic cell-mediated immunoglobulin A enhancement.
    Authors: Kotani Y, Kunisawa J, Suzuki Y, Sato I, Saito T, Toba M, Kohda N, Kiyono H
    PLoS ONE, 2014-03-14;9(3):e91857.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  21. The development and survival but not function of follicular B cells is dependent on IL-7Ralpha Tyr449 signaling.
    Authors: Patton D, Plumb A, Redpath S, Osborne L, Perona-Wright G, Abraham N
    PLoS ONE, 2014-02-13;9(2):e88771.
    Species: Mouse
    Sample Types: Serum
  22. Oral administration of Lactobacillus plantarum strain AYA enhances IgA secretion and provides survival protection against influenza virus infection in mice.
    Authors: Kikuchi, Yosuke, Kunitoh-Asari, Ayami, Hayakawa, Katsuyuk, Imai, Shinjiro, Kasuya, Kenji, Abe, Kimio, Adachi, Yu, Fukudome, Shin-Ich, Takahashi, Yoshimas, Hachimura, Satoshi
    PLoS ONE, 2014-01-22;9(1):e86416.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  23. Recombination-activating gene 1 (Rag1)-deficient mice with severe combined immunodeficiency treated with lentiviral gene therapy demonstrate autoimmune Omenn-like syndrome.
    Authors: van Til N, Sarwari R, Visser T, Hauer J, Lagresle-Peyrou C, van der Velden G, Malshetty V, Cortes P, Jollet A, Danos O, Cassani B, Zhang F, Thrasher A, Fontana E, Poliani P, Cavazzana M, Verstegen M, Villa A, Wagemaker G
    J Allergy Clin Immunol, 2013-12-09;133(4):1116-23.
    Species: Mouse
    Sample Types: Plasma
  24. Inhibition of hedgehog signaling for the treatment of murine sclerodermatous chronic graft-versus-host disease.
    Blood, 2012-08-22;120(14):2909-17.
    Species: Mouse
    Sample Types: Serum
  25. Lack of T cells in Act1-deficient mice results in elevated IgM-specific autoantibodies but reduced lupus-like disease.
    Authors: Johnson AC, Davison LM, Giltiay NV, Vareechon C, Li X, Jorgensen TN
    Eur. J. Immunol., 2012-07-01;42(7):1695-705.
    Species: Mouse
    Sample Types: Serum
  26. Mice overexpressing BAFF develop a commensal flora-dependent, IgA-associated nephropathy.
    Authors: McCarthy DD, Kujawa J, Wilson C
    J. Clin. Invest., 2011-09-01;121(10):3991-4002.
    Species: Mouse
    Sample Types: Serum
  27. The adaptor molecule Act1 regulates BAFF responsiveness and self-reactive B cell selection during transitional B cell maturation.
    Authors: Giltiay NV, Lu Y, Allman D, Jorgensen TN, Li X
    J. Immunol., 2010-06-11;185(1):99-109.
    Species: Mouse
    Sample Types: Serum
  28. Secreted IgM enhances B cell receptor signaling and promotes splenic but impairs peritoneal B cell survival.
    Authors: Notley CA, Baker N, Ehrenstein MR
    J. Immunol., 2010-03-05;184(7):3386-93.
    Species: Mouse
    Sample Types: Serum
  29. Emu-BCL10 mice exhibit constitutive activation of both canonical and noncanonical NF-kappaB pathways generating marginal zone (MZ) B-cell expansion as a precursor to splenic MZ lymphoma.
    Authors: Li Z, Wang H, Xue L, Shin DM, Roopenian D, Xu W, Qi CF, Sangster MY, Orihuela CJ, Tuomanen E, Rehg JE, Cui X, Zhang Q, Morse HC, Morris SW
    Blood, 2009-08-20;114(19):4158-68.
    Species: Mouse
    Sample Types: Serum
  30. Prevention of murine antiphospholipid syndrome by BAFF blockade.
    Authors: Kahn P, Ramanujam M, Bethunaickan R, Huang W, Tao H, Madaio MP, Factor SM, Davidson A
    Arthritis Rheum., 2008-09-01;58(9):2824-34.
    Species: Mouse
    Sample Types: Serum

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