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Mouse BCMA/TNFRSF17 Antibody

Catalog #: MAB593
1 Citation Datasheet / COA / SDS
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MAB593
MAB593-SP
Product Details
Citations (1)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse BCMA/TNFRSF17 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse BCMA in direct ELISAs. In direct ELISAs, this antibody does not cross-react with recombinant human (rh) BCMA or rhTACI.
Source
Monoclonal Rat IgG1 Clone # 161616
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse BCMA
Met1-Thr49
Accession # O88472
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Blockade of Receptor-ligand Interaction
In a functional ELISA, 2 - 8 μg/mL of this antibody will block 50% of the binding of 750 ng/mL of recombinant human APRIL to immobilized recombinant mouse BCMA/Fc Chimera coated at 1 μg/mL (100 μL/well). At 100 μg/mL, this antibody will block >90% of the binding. The specific conditions are described in the figure legend. In this application, the use of anti-mouse BCMA monoclonal antibody (Catalog # MAB5931), is recommended.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BCMA/TNFRSF17

BCMA, B cell maturation, is a member of the TNF receptor superfamily. It has been designated TNFRSF17. BCMA is a type III membrane protein containing one extracellular cysteine rich domain. Within the TNFRSF, it shares the highest homology with TACI. BCMA and TACI have both been shown to bind to APRIL and BAFF, members of the TNF ligand superfamily. BCMA expression has been found in immune organs and mature B cell lines. Although some expression has been observed at the cell surface, BCMA appears to be localized to the Golgi compartment. The binding of BCMA to APRIL or BAFF has been shown to stimulate IgM production in peripheral blood B cells and increase the survival of cultured B cells. This data suggests that BCMA may play an important role in B cell development, function and regulation. Mouse BCMA is a 185 amino acid (aa) protein consisting of a 49 aa extracellular domain, a 23 aa transmembrane domain, and a 113 aa intracellular domain. Mouse and human BCMA share 62% amino acid identity.

References
  1. Madry, C. et al. (1998) Int. Immunol. 10:1693.
  2. Gras, M. et al. (1995) Int. Immunol. 7:1093.
  3. Kwon, B. et al. (1999) Curr. Opin. Immunol. 11:340.
  4. Marsters, S. et al. (2000) Curr. Biol. 10:785.
  5. Thompson, J. et al. (2000) J. Exp. Med. 192:129.
Long Name
B Cell Maturation Factor
Entrez Gene IDs
608 (Human); 21935 (Mouse); 287034 (Rat); 102145399 (Cynomolgus Monkey)
Alternate Names
B cell maturation antigen; B-cell maturation protein; BCMA; BCMAtumor necrosis factor receptor superfamily member 17; BCMB-cell maturation factor; CD269 antigen; CD269; TNFRSF13A; TNFRSF17; tumor necrosis factor receptor superfamily, member 17

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Citation for Mouse BCMA/TNFRSF17 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. B cell maturation antigen, the receptor for a proliferation-inducing ligand and B cell-activating factor of the TNF family, induces antigen presentation in B cells.
    Authors: Yang M, Hase H, Legarda-Addison D, Varughese L, Seed B, Ting AT
    J. Immunol., 2005-09-01;175(5):2814-24.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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