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Mouse BCMA/TNFRSF17 Antibody

Catalog #: AF593
3 Citations Datasheet / COA / SDS
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AF593
AF593-SP
Product Details
Citations (3)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse BCMA/TNFRSF17 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects BCMA/TNFRSF17 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant human BCMA is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse BCMA/TNFRSF17
Met1-Thr49
Accession # O88472
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse BCMA/TNFRSF17 Fc Chimera (Catalog # 593-BC)
Blockade of Receptor-ligand Interaction
In a functional ELISA, 1-4 µg/mL of this antibody will block 50% of the binding of 750 ng/mL of Recombinant Human APRIL/TNFSF13 (Catalog # 884-AP) to immobilized Recombinant Mouse BCMA/TNFRSF17 Fc Chimera (Catalog # 593-BC) coated at 1 µg/mL (100 µL/well). At 25 μg/mL, this antibody will block >90% of the binding.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BCMA/TNFRSF17

BCMA, B cell maturation, is a member of the TNF receptor superfamily. It has been designated TNFRSF17. BCMA is a type III membrane protein containing one extracellular cysteine rich domain. Within the TNFRSF, it shares the highest homology with TACI. BCMA and TACI have both been shown to bind to APRIL and BAFF, members of the TNF ligand superfamily. BCMA expression has been found in immune organs and mature B cell lines. Although some expression has been observed at the cell surface, BCMA appears to be localized to the Golgi compartment. The binding of BCMA to APRIL or BAFF has been shown to stimulate IgM production in peripheral blood B cells and increase the survival of cultured B cells. This data suggests that BCMA may play an important role in B cell development, function and regulation. Mouse BCMA is a 185 amino acid (aa) protein consisting of a 49 aa extracellular domain, a 23 aa transmembrane domain, and a 113 aa intracellular domain. Mouse and human BCMA share 62% amino acid identity.

References
  1. Madry, C. et al. (1998) Int. Immunol. 10:1693.
  2. Gras, M. et al. (1995) Int. Immunol. 7:1093.
  3. Kwon, B. et al. (1999) Curr. Opin. Immunol. 11:340.
  4. Marsters, S. et al. (2000) Curr. Biol. 10:785.
  5. Thompson, J. et al. (2000) J. Exp. Med. 192:129.
Long Name
B Cell Maturation Factor
Entrez Gene IDs
608 (Human); 21935 (Mouse); 287034 (Rat); 102145399 (Cynomolgus Monkey)
Alternate Names
B cell maturation antigen; B-cell maturation protein; BCMA; BCMAtumor necrosis factor receptor superfamily member 17; BCMB-cell maturation factor; CD269 antigen; CD269; TNFRSF13A; TNFRSF17; tumor necrosis factor receptor superfamily, member 17

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Citations for Mouse BCMA/TNFRSF17 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. The BAFF receptor TACI controls IL-10 production by regulatory B cells and CLL B cells.
    Authors: Saulep-Easton D, Vincent F, Quah P, Wei A, Ting S, Croce C, Tam C, Mackay F
    Leukemia, 2015-07-03;30(1):163-72.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  2. Functional implication of BAFF synthesis and release in gangliosides-stimulated microglia.
    Authors: Kim KS, Park JY, Jou I, Park SM
    J. Leukoc. Biol., 2009-04-30;86(2):349-59.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. B cell maturation antigen, the receptor for a proliferation-inducing ligand and B cell-activating factor of the TNF family, induces antigen presentation in B cells.
    Authors: Yang M, Hase H, Legarda-Addison D, Varughese L, Seed B, Ting AT
    J. Immunol., 2005-09-01;175(5):2814-24.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Functional Assay

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