Mouse beta IG-H3 Antibody

Catalog #: AF2559 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2559
AF2559-SP

1 Image

All beta IG-H3 Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse beta IG-H3 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse beta IG-H3 in ELISAs and Western blots. In sandwich immunoassays, less than 6% cross-reactivity with recombinant human (rh) beta IG-H3 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse beta IG-H3
Gly24-His683
Accession # P82198

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse beta IG-H3 (Catalog # 2559-BG)

Immunohistochemistry

5-15 µg/mL

See below

Mouse beta IG-H3 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

0.2-0.8 µg/mL

Use in combination with:

Detection Reagent: Mouse beta IG‑H3 Biotinylated Antibody (Catalog # BAF2559)

Standard: Recombinant Mouse beta IG-H3 Protein, CF (Catalog # 2559-BG)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

View Larger

beta IG‑H3 in Mouse Embryo. beta IG-H3 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Sheep Anti-Mouse beta IG-H3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2559) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to developing duodenum. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: beta IG-H3

Beta IG-H3, also known as TGFBI and RGD-CAP, is a matricellular adaptor protein that is induced in most cell types in response to TGF-beta stimulation (1‑4). The mouse beta IG-H3 cDNA encodes a 683 amino acid (aa) precursor that includes a 23 aa signal sequence, one EMI domain, four FAS1 domains, and one RGD motif (2). Mouse beta IG-H3 shares 91% aa sequence identity with human and porcine beta IG-H3. beta IG-H3 is expressed as a 75 kDa protein with no post-translational additions (5). Following secretion, cleavages at multiple positions near the C-terminal end liberate peptides with pro-apoptotic activity (5, 6). Peptides that encompass the RGD motif contribute to the pro-apoptotic effects of TGF-beta (6). FAS1 domains contain YH motifs that are characterized by conserved Tyr and His residues (7). The YH motifs in each of the FAS1 domains enable beta IG-H3 to bind to matrix fibronectin, collagen I, collagen VI, biglycan, and decorin (3, 8‑11), in addition to cell expressed integrins alpha V/ beta 3, alpha V beta 5, and alpha 3 beta 1 (7, 8, 12, 13). The expression of beta IG-H3 is modulated at particular developmental stages in some cell types. It is upregulated in keratinocytes and immature dendritic cells but downregulated in osteoblasts (8, 12, 14). It promotes keratinocyte differentiation but blocks osteoblast differentiation (8, 12). beta IG-H3 stimulates macrophage endocytosis and vascular endothelial cell proliferation and migration (13, 14). High glucose levels induce beta IG-H3 in renal proximal tubule cells which is predictive of diabetic nephropathy (3). Several point mutations (clustered in the fourth FAS1 domain) of beta IG-H3 are linked to different corneal dystrophies (15). beta IG-H3 is downregulated in many cancers (4, 16) and functions as a suppressor of tumorigenicity when overexpressed (2, 4, 16).

References

Skonier, J. et al. (1992) DNA Cell Biol. 11:511.
Skonier, J. et al. (1994) DNA Cell Biol. 13:571.
Lee, S-H. et al. (2003) Kidney Int. 64:1012.
Zhao, Y.L. et al. (2002) Oncogene 21:7471.
Andersen, R.B. et al. (2004) Biochemistry 43:16374.
Kim, J-E. et al. (2003) Oncogene 22:2045.
Kim, J-E. et al. (2002) J. Biol. Chem. 277:46159.
Thapa, N. et al. (2005) Bone 36:232.
Hanssen, E. et al. (2003) J. Biol. Chem. 278:24334.
Billings, P.C. et al. (2002) J. Biol. Chem. 277:28003.
Reinboth, B. et al. (2006) J. Biol. Chem. 281:7816.
Oh, J-E. et al. (2005) J. Biol. Chem. 280:21629.
Nam, J-O. et al. (2003) J. Biol. Chem. 278:25902.
Cao, W. et al. (2006) Blood 107:2777.
Stewart, H.S. et al. (1999) Hum. Mutat. 14:126.
Zhao, Y. et al. (2006) Mol. Carcinog. 45:84.

Long Name

TGF-beta Induced Gene H3

Entrez Gene IDs

7045 (Human); 21810 (Mouse)

Alternate Names

beta IGH3; beta IG-H3; BIGH3EBMD; CDB1; CDG2; CDGG1transforming growth factor, beta-induced, 68kD; CSD; CSD1; CSD2; CSD3; Kerato-epithelin; LCD1; RGD-CAP; RGD-containing collagen-associated protein; TGFBI; TGFBIP; transforming growth factor, beta-induced, 68kDa; transforming growth factor-beta-induced protein ig-h3