Mouse BMP-6 Antibody

Catalog #: AF6325 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6325
AF6325-SP

Detection of Mouse BMP‑6 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse BMP-6 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects recombinant human and mouse BMP-6 in direct ELISAs and detects mouse BMP-6 in Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse (rm) BMP-5 and rmBMP-7 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse BMP-6
Ser372-His510
Accession # P20722

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse BMP‑6 by Western Blot. Western blot shows lysates of NS0 mouse myeloma cell line either mock transfected or transfected with mouse BMP-6. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse BMP-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6325) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). For additional reference, Recombinant Mouse BMP-6 (Catalog # 6325-BM) (10ng/lane) was included. A specific band was detected for BMP-6 at approximately 20kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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BMP‑6 in Mouse Embryo. BMP-6 was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Sheep Anti-Mouse BMP-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6325) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to skeletal muscle cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BMP-6

Bone Morphogenetic Protein 6 (BMP‑6), also known as Vgr-1, is one of at least 15 structurally and functionally related BMPs which are members of the transforming growth factor beta (TGF-beta ) superfamily. Mouse BMP‑6 is synthesized as a 510 amino acid (aa) precursor protein that is cleaved at the dibasic cleavage site (RxxR) to release the 18 kDa C‑terminal mature protein. Biologically active BMP‑6 consists of a disulfide-linked homodimer of the mature proteins (1, 2). Mature mouse BMP‑6 shares 96% and 98% aa sequence identity with human and rat BMP‑6, respectively. Cellular responses to BMP‑6 are mediated by hetero-oligomeric complexes of type I (Activin RIA/ALK-2 and BMPR-IA/ALK-3) and type II (Activin RIIA and BMPR-II) serine/threonine kinase receptors (3‑5). Glycosylation of BMP‑6 is required for its interaction with Activin RIA (6). BMP‑6 induces the expression of Noggin and is subsequently antagonized by Noggin (7, 8). BMP‑6 induces a wide range of cellular responses. It promotes osteoblast differentiation from mesenchymal stem cells (5), chondrocyte maturation (9), Ang II-induced aldosterone production in the adrenal cortex (3), hormone production and responsiveness in ovarian granulosa cells (10), iNOS and TNF-alpha production in macrophages (4), the cell death of B cells (8), and neurite outgrowth (11). BMP‑6 expression is induced in astrocytes surrounding sites of brain injury where it functions as a neuroprotectant (11, 12). BMP‑6 is upregulated during prostate cancer progression and promotes tumor cell metastasis to bone (13). Through interactions with the BMP coreceptor RGM‑C/Hemojuvelin, BMP‑6 plays an important role in iron homeostasis by promoting Hepcidin expression and preventing serum iron overload (14, 15).

References

Chen, D. et al. (2004) Growth Factors 22:233.
Lyons, K. et al. (1989) Proc. Natl. Acad. Sci. 86:4554.
Inagaki, K. et al. (2006) Endocrinology 147:2681.
Hong, J.H. et al. (2008) Immunology 128:e442.
Lavery, K. et al. (2008) J. Biol. Chem. 283:20948.
Saremba, S. et al. (2008) FEBS J. 275:172.
Haudenschild, D.R. et al. (2004) Cancer Res. 64:8276.
Kersten, C. et al. (2005) BMC Immunol. 6:9.
Grimsrud, C.D. et al. (1999) J. Bone Miner. Res. 14:475.
Shi, J. et al. (2009) Fertil. Steril. 92:1794.
Yabe, T. et al. (2002) J. Neurosci. Res. 68:161.
Zhang, Z. et al. (2006) Neuroscience 138:47.
Dai, J. et al. (2005) Cancer Res. 65:8274.
Meynard, D. et al. (2009) Nat. Genet. 41:478.
Andriopoulos, B. Jr. et al. (2009) Nat. Genet. 41:482.

Long Name

Bone Morphogenetic Protein 6

Entrez Gene IDs

654 (Human); 12161 (Mouse); 25644 (Rat)

Alternate Names

BMP6; BMP-6; bone morphogenetic protein 6; vegetal related growth factor (TGFB-related); vegetal-related (TGFB related) cytokine; VG-1-R; VG-1-related protein; Vg1-related sequence; VGR; VGR1; Vgr-1