Mouse BTNL2/Butyrophilin-like 2 Antibody

Catalog #: AF5236 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5236
AF5236-SP

Detection of Mouse BTNL2/Butyrophilin-like 2 by Western Blot.

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Mouse BTNL2/Butyrophilin-like 2 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse BTNL2/Butyrophilin-like 2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross‑reactivity with recombinant mouse (rm) BTN, rmBTN2, and recombinant human BTN is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse BTNL2/Butyrophilin-like 2
aa 27-452
Accession # O70355

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse BTNL2/Butyrophilin-like 2 by Western Blot. Western blot shows lysates of mouse small intestine tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Mouse BTNL2/Butyrophilin-like 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5236) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for BTNL2/Butyrophilin-like 2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BTNL2/Butyrophilin-like 2

Butyrophilin-like 2 (BTNL2; also BTL-II) is a predicted 60‑65 kDa member of the BTN/MOG family, Ig-superfamily of proteins. In mouse, it is expressed in intestinal villi columnar epithelial cells and colonic dendritic cells. BTNL2 apparently blocks CD4+ T cell activation. Mouse BTNL2 is a 454 amino acid (aa) type II transmembrane glycoprotein. It contains a 431 aa extracellular region (aa 24‑454) with four V-type Ig-like domains. There are three splice variants. One shows a deletion of the two C-terminal amino acids, a second shows a deletion of aa 237‑243, while a third shows a C-terminal 62 substitution for Gly453Trp454. Over aa 27‑452, mouse BTNL2 shares 88% and 64% aa identity with rat and human BTNL2, respectively.

Entrez Gene IDs

56244 (Human); 547431 (Mouse)

Alternate Names

BTL-II; BTNL2; Butyrophilin like 2; butyrophilin-like 2 (MHC class II associated); Butyrophilin-like 2