Mouse C5L2/GPR77 Antibody

Catalog #: MAB4729
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4729
MAB4729-SP

Detection of C5L2/GPR77 in RAW 264.7 Mouse Cell Line by Flow Cytometry.

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All C5L2/GPR77 Antibodies

Product Details

Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse C5L2/GPR77 Antibody Summary

Species Reactivity

Mouse

Specificity

This antibody stains mouse C5L2 transfectants but not irrelevant transfectants.

Source

Monoclonal Rat IgG_2B Clone # 468705

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line transfected with mouse C5L2
Met1-Val344
Accession # Q8BW93

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of C5L2/GPR77 antibody in RAW 264.7 Mouse Cell Line antibody by Flow Cytometry.

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Detection of C5L2/GPR77 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse C5L2/GPR77 Monoclonal Antibody (Catalog # MAB4729, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: C5L2/GPR77

C5L2, also known as GPR77, is a 45 kDa 7TM receptor that binds the complement proteins C3a, C4a, and C5a as well as the acylation stimulating peptide (ASP). C5L2 is expressed on various hematopoietic cells where it promotes complement-mediated inflammation. ASP binding to C5L2 on adipocytes triggers triglyceride synthesis and glucose uptake and inhibits lipolysis. Human C5L2 shares 60% amino acid sequence identity with mouse and rat C5L2.

Long Name

C5a Anaphylatoxin Chemotactic Receptor C5L2

Entrez Gene IDs

27202 (Human); 319430 (Mouse); 445269 (Rat)

Alternate Names

C5a anaphylatoxin chemotactic receptor C5L2; C5L2; C5L2G-protein coupled receptor 77; G protein-coupled receptor 77; G protein-coupled receptor C5L2; GPF77; GPR77

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citations for Mouse C5L2/GPR77 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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T Cell Expression of C5a Receptor 2 Augments Murine Regulatory T Cell (T) Generation and T-Dependent Cardiac Allograft Survival
Authors: D A Verghese, M Demir, N Chun, M Fribourg, P Cravedi, I Llaudo, TM Woodruff, P Yadav, SA Lira, ME Medof, PS Heeger
J. Immunol., 2018-02-07;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
Monitoring C5aR2 Expression Using a Floxed tdTomato-C5aR2 Knock-In Mouse
Authors: CM Karsten, AV Wiese, F Mey, J Figge, TM Woodruff, T Reuter, O Scurtu, A Kordowski, LN Almeida, D Briukhovet, KM Quell, J Sun, F Ender, I Schmudde, T Vollbrandt, Y Laumonnier, J Köhl
J. Immunol., 2017-09-01;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry