Mouse Carbonic Anhydrase XII/CA12 Antibody

Catalog #: MAB2345 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2345
MAB2345-SP

All Carbonic Anhydrase XII/CA12 Antibodies

Product Details

Supplemental Products

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Carbonic Anhydrase XII/CA12 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Carbonic Anhydrase XII/CA12 in direct ELISAs and Western blots. In Western blots, 100% cross‑reactivity with recombinant human (rh) CA12, 10% cross-reactivity with rhCA2, and no cross-reactivity with rhCA1, 3, 5A, 5B, 6, 7, 8, 10, 11, 13, recombinant mouse CA4, 9, or 14 is observed.

Source

Monoclonal Rat IgG_2A Clone # 336103

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Carbonic Anhydrase XII/CA12
Ala25-Ser301
Accession # Q8CI85

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse Carbonic Anhydrase XII (Catalog # 2345-CA)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Carbonic Anhydrase XII/CA12

Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO₂ + H₂O = HCO₃^- + H⁺, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Mouse CA12 is a type I membrane enzyme with both CA activity and esterase activity (2, 3). The amino acid sequence of mouse CA12 shares 97%, 82%, and 68% identity with that of rat, human/chimpanzee/canine, and chicken.

References

Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
Strausberg, R.L. et al. (2002) Proc. Natl. Acad. Sci. USA 99:16899.
Okazaki, Y. et al. (2002) Nature 420:563.

Entrez Gene IDs

771 (Human); 76459 (Mouse)

Alternate Names

CA12; Carbonate dehydratase XII; carbonic anhydrase 12; Carbonic Anhydrase XII; carbonic anhydrase XIICAXII; carbonic dehydratase; CA-XII; EC 4.2.1.1; FLJ20151; HsT18816; Tumor antigen HOM-RCC-3.1.3