Mouse Cardiotrophin-1/CT-1 Antibody

Catalog #: AF438
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF438
AF438-SP

Cell Proliferation Induced by Cardiotrophin‑1/CT‑1 and Neutralization by Mouse Cardiotrophin‑1/CT‑1 Antibody.

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Citations (2)

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Mouse Cardiotrophin-1/CT-1 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects Cardiotrophin‑1/CT‑1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 40% cross-reactivity with recombinant human CT-1 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse Cardiotrophin-1/CT‑1
Ser2-Ala203
Accession # Q60753

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse Cardiotrophin‑1/CT‑1 (Catalog # 438-CT)

Neutralization

Measured by its ability to neutralize Cardiotrophin‑1/CT‑1-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND₅₀) is typically 0.08-0.48 µg/mL in the presence of 10 ng/mL Recombinant Mouse Cardiotrophin‑1/CT‑1.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Cell Proliferation Induced by Cardiotrophin‑1/CT‑1 and Neutralization by Mouse Cardiotrophin‑1/CT‑1 Antibody. Recombinant Mouse Cardiotrophin-1/CT-1 (Catalog # 438-CT) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse Cardiotrophin-1/CT-1 (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse Cardiotrophin-1/CT-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF438). The ND₅₀ is typically 0.08-0.48 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cardiotrophin-1/CT-1

Cardiotrophin-1 (CT-1) is a member of the cytokine family which also includes IL-6, IL-11, leukemia inhibitory factor (LIF), oncostatin M (OSM), and ciliary neurotrophic factor (CNTF). It was originally isolated based on its ability to induce cardiac myocyte hypertrophy in vitro. CT-1 has since been shown to be a pleiotrophic cytokine with overlapping actions with other IL-6 family members on a variety of cell types. Mouse CT-1 encodes a 203 amino acid (aa) residue protein that lacks a hydrophobic signal peptide. The mechanism of CT-1 release from cells is currently not understood. Human and mouse CT-1 share 80% aa sequence identity and exhibit cross-species activity. CT-1 is highly expressed in heart, skeletal muscle, liver, lung and kidney. Lower levels of CT-1 expression is also seen in testis and brain. CT-1 initiates downstream signaling pathways through the heterodimerization of gp130 and the LIF receptor beta subunit. A third alpha receptor subunit has also been implicated in the receptor complex.

References

Pennica, D. et al. (1996) Cytokine and Growth Factor Reviews 7:81.
Robledo, O. et al. (1997) J. Biol. Chem. 272:4855.

Entrez Gene IDs

1489 (Human); 13019 (Mouse)

Alternate Names

cardiophin 1; cardiotrophin 1; Cardiotrophin1; Cardiotrophin-1; CT-1CT1; CTF1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse Cardiotrophin-1/CT-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Cardiotrophin-1 defends the liver against ischemia-reperfusion injury and mediates the protective effect of ischemic preconditioning.
Authors: Iniguez M, Berasain C, Martinez-Anso E, Bustos M, Fortes P, Pennica D, Avila MA, Prieto J
J. Exp. Med., 2006-12-18;203(13):2809-15.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization
In vivo electrotransfer of the cardiotrophin-1 gene into skeletal muscle slows down progression of motor neuron degeneration in pmn mice.
Authors: Lesbordes JC, Bordet T, Haase G, Castelnau-Ptakhine L, Rouhani S, Gilgenkrantz H, Kahn A
Hum. Mol. Genet., 2002-07-01;11(14):1615-25.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA Development