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Mouse Cathepsin 7/Cathepsin 1 Antibody

Catalog #: MAB1499
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1499
MAB1499-SP
Product Details
Citations (1)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Cathepsin 7/Cathepsin 1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Cathepsin 7/Cathepsin 1 in direct ELISAs and Western blots. In Western blots, no cross‑reactivity with recombinant human Cathepsin O, S, or V, or recombinant mouse Cathepsin B, C, H, L, or Z is observed.
Source
Monoclonal Rat IgG2A Clone # 228905
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Cathepsin 7/Cathepsin 1
Ala18-Leu331
Accession # Q91ZF2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse Cathepsin 7/Cathepsin 1
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Mouse Cathepsin 7/Cathepsin 1, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cathepsin 7/Cathepsin 1

Mouse Cathepsin 7, also known as Cathepsin 1, is a cysteine protease encoded by one of the genes in the cluster on mouse chromosome 13 that is absent from the human genome. It is expressed only in the placenta, suggesting a role of the gene cluster in formation and development of murine placenta.

Entrez Gene IDs
56092 (Mouse); 290970 (Rat)
Alternate Names
Cathepsin 1; Cathepsin 7; CTS1; CTS7; Epcs24; Epcs71

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Citation for Mouse Cathepsin 7/Cathepsin 1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Plet1 is an epigenetically regulated cell surface protein that provides essential cues to direct trophoblast stem cell differentiation
    Authors: Alexander Murray, Arnold R. Sienerth, Myriam Hemberger
    Scientific Reports

FAQs

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