Mouse Ccd1/DIXDC1 Antibody Summary
Gln481-Asn711
Accession # Q80Y83
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Mouse Ccd1/DIXDC1 by Western Blot. Western blot shows lysates of mouse brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Ccd1/DIXDC1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5599) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Specific bands were detected for Ccd1/DIXDC1 at approximately 59 and 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
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Ccd1/DIXDC1 in Mouse Embryo. Ccd1/DIXDC1 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Mouse Ccd1/DIXDC1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5599) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to neopallial cortex and midbrain. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Ccd1/DIXDC1
Coiled-coil-DIX1 (Ccd1), also known as DIXDC1, is an 80 kDa cytoplasmic actin-binding protein that mediates Wnt signaling by interacting with Dishevelled and Axin. It is highly expressed during nervous system development. Mouse Ccd1 contains a CH domain (aa 22‑152), a coiled-coil domain (aa 279‑452), and a DIX domain (aa 629‑705). Multiple alternately spliced isoforms of mouse Ccd11 are expressed in a tissue specific manner during development and in the adult. These variants may lack approximately one third of the N-terminal region of the molecule or carry short deletions within the CH domain or spacer region between the coiled-coil and DIX domains. Over aa 481‑711, mouse Ccd1 shares 88% and 75% aa sequence identity with human and rat Ccd1, respectively.
Product Datasheets
Citation for Mouse Ccd1/DIXDC1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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A novel AMPK-independent signaling pathway downstream of the LKB1 tumor suppressor controls Snail1 and metastatic potential
Authors: Jonathan M. Goodwin, Robert U. Svensson, Hua Jane Lou, Monte M. Winslow, Benjamin E. Turk, Reuben J. Shaw
Molecular Cell
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