Mouse CCDC134 Antibody

Catalog #: MAB7786 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7786
MAB7786-SP

Detection of Mouse CCDC134 by Western Blot.

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All CCDC134 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CCDC134 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CCDC134 in direct ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant human CCDC134 is observed.

Source

Monoclonal Rat IgG_2A Clone # 815606

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CCDC134
Ala23-Leu229
Accession # Q8C7V8

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse CCDC134 by Western Blot. Western blot shows lysates of J774A.1 mouse reticulum cell sarcoma macrophage cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse CCDC134 Monoclonal Antibody (Catalog # MAB7786) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for CCDC134 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCDC134

Coiled-coil domain containing 134 (CCDC134) is a widely expressed glycoprotein that is secreted as a 38 kDa molecule. CCDC134 overexpression inhibits kinase activity in the Raf-1/MEK/ERK and JNK/SAPK pathways. In humans, CCDC134 polymorphisms are associated with reduced levels of Amyloid beta peptide (1-42) in the cerebrospinal fluid, a potential biomarker for Alzheimer’s disease. Alternative splicing of mouse CCDC134 generates a short isoform that lacks the N-terminal 37 amino acids including the signal peptide. Mouse CCDC134 shares 90% and 95% aa sequence identity with human and rat CCDC134, respectively.

Long Name

Coiled-coil Domain Containing 134

Entrez Gene IDs

79879 (Human); 76457 (Mouse); 500909 (Rat)

Alternate Names

CCDC134; coiled-coil domain containing 134; coiled-coil domain-containing protein 134; dJ821D11.3; FLJ22349; MGC21013