Mouse CCL21/6Ckine Quantikine ELISA Kit

Catalog #: M6C00
1 Citation Datasheet / COA / SDS

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M6C00

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All CCL21/6Ckine ELISAs

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Mouse CCL21/6Ckine Quantikine ELISA Kit Summary

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)

Sensitivity

0.489 pg/mL

Assay Range

7.8 - 500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant mouse 6Ckine.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Mouse CCL21/6Ckine Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse 6Ckine in tissue culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse 6Ckine and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant mouse 6Ckine. Results obtained using natural mouse 6Ckine showed dose response curves that were parallel to the standard curves obtained using the Quantikine mouse kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse 6Ckine.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	70.4	145	315	70.1	138	295
Standard Deviation	2.96	4.3	13.9	4.79	8.72	15.8
CV%	4.2	3	4.4	6.8	6.3	5.4

Recovery

The recovery of mouse 6Ckine spiked to levels throughout the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	96	88-104

Linearity

To assess the linearity of the assay, samples containing high concentrations of mouse 6Ckine were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Scientific Data

N/A

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Mouse CCL21/6Ckine ELISA Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CCL21/6Ckine

CCL21, also known as 6Ckine, TCA-4, SLC, Exodus-2, and A21, is a homeostatic chemokine that signals through CCR7 or CXCR3. CCL21 is constitutively presented on initial lymphatic vessels, high endothelial venules (HEV), and lymph node dendritic cells (DC) where it promotes the docking of DC to lymphatic vessels and the retention of T cells by lymph node DC, resulting in T cell priming for activation. Its upregulation during chronic inflammation or tissue damage promotes fibrosis, inflammatory cytokine production, and neuropathic pain. The soluble chemokine is elevated in rheumatoid arthritis synovial fluid and in the serum of coronary artery disease patients.

Entrez Gene IDs:

6366 (Human); 18829 (Mouse); 298006 (Rat)

Alternate Names:

6Ckine; 6CkineSmall-inducible cytokine A21; Beta-chemokine exodus-2; CCL21; chemokine (C-C motif) ligand 21; CKb9; exodus-2; member 21; SCYA21; SCYA21MGC34555; secondary lymphoid tissue chemokine; SLC; SLCSecondary lymphoid-tissue chemokine; TCA-4

Related Research Areas

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citation for Mouse CCL21/6Ckine Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Drainage of senescent astrocytes from brain via meningeal lymphatic routes
Authors: Q Li, Y Chen, W Feng, J Cai, J Gao, F Ge, T Zhou, Z Wang, F Ding, C Marshall, C Sheng, Y Zhang, M Sun, J Shi, M Xiao
Brain, Behavior, and Immunity, 2022-04-12;103(0):85-96.
Species: Mouse
Sample Types: Tissue Homogenates