Mouse CCL22/MDC Antibody

Catalog #: MAB439
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB439
MAB439-SP

All CCL22/MDC Antibodies

Product Details

Citations (1)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CCL22/MDC Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CCL22/MDC in direct ELISAs and Western blots. Shows 12% cross-reactivity with recombinant human (rh) CCL22 and recombinant mouse (rm) MIP-3 alpha, 25% cross-reactivity with rmCTACK, 6% cross-reactivity with rhBLC/BCA-1, and no cross-reactivity with rcrMIP-1 alpha, rhMIP-1 alpha, rmMIP-1 alpha, rcrMIP-1 beta, rmMIP-1 beta, rhMIP-1 beta rhPARC, rcrRANTES, rhRANTES, rmRANTES, rhTARC, or rmTARC,

Source

Monoclonal Rat IgG_2A Clone # 158113

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant mouse CCL22/MDC
Gly25-Ser92
Accession # O88430

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse CCL22/MDC (Catalog # 439-MD)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL22/MDC

Mouse ABCD-1, the mouse orthologue of the human macrophage-derived chemokine (MDC)/stimulated T cell chemotactic protein (STCP-1), is a CC chemokine cloned from activated mouse B cells. Mouse MDC cDNA encodes a precursor protein of 92 amino acid (aa) residues with a 24 aa residue predicted signal peptide that is cleaved to yield a 68 aa residue mature 7.8 kDa protein. At the amino acid sequence level, mouse and human MDC share 64% identity and 83% similarity. The genomic organization of the mouse and human MDC genes are very similar, exhibiting sequence identity at the intron-exon boundaries. Mouse MDC is expressed at high levels in dendritic cells and activated B lymphocytes. Low levels of mouse MDC mRNA are also detectable in lung, unstimulated spleen cells, lymph node cells and in thymocytes. MDC is a functional ligand for the CC chemokine receptor 4. Recombinant or chemically synthesized mature mouse MDC has been shown to induce chemotaxis or Ca²⁺ mobilization in activated mouse and human T cells.

References

Schaniel, C. et al. (1998) J. Exp. Med. 188:451.
Imai, T. et al. (1998) J. Biol. Chem. 273:1764.
Godiska, R. et al. (1997) J. Exp. Med. 185:1595.
Chang, M-S. et al. (1997) J. Biol. Chem. 272:25229.

Entrez Gene IDs

6367 (Human); 20299 (Mouse)

Alternate Names

A-152E5.1; ABCD-1; CC chemokine STCP-1; C-C motif chemokine 22; CCL22; chemokine (C-C motif) ligand 22; DC/B-CK; Macrophage-derived chemokine; MDC; MDCStimulated T-cell chemotactic protein 1; MGC34554; SCYA22MDC(1-69); small inducible cytokine A22; small inducible cytokine subfamily A (Cys-Cys), member 22; Small-inducible cytokine A22; STCP-1; stimulated T cell chemotactic protein 1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Mouse CCL22/MDC Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

CD4+CD25+ regulatory T cells reverse established allergic airway inflammation and prevent airway remodeling.
Authors: Kearley J, Robinson DS, Lloyd CM
J. Allergy Clin. Immunol., 2008-07-30;122(3):617-24.e6.
Species: Mouse
Sample Types: BALF
Applications: ELISA Development