Mouse CCL3/MIP-1 alpha Biotinylated Antibody

Name: Mouse CCL3/MIP-1 alpha Biotinylated Antibody
Brand: R&D Systems
SKU: BAF450
Rating: 4.7 (3 reviews)

Catalog #: BAF450
12 Citations 3 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
BAF450

All CCL3/MIP-1 alpha Antibodies

Product Details

Citations (12)

FAQs

Supplemental Products

Reviews (3)

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CCL3/MIP-1 alpha Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse MIP-1 alpha in ELISAs and Western blots. In sandwich immunoassays, less than 0.05% cross‑reactivity with rhCCL3, rmCCL9/10 and rmCCL4 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse CCL3/MIP-1 alpha (R&D Systems, Catalog # 450-MA)
Ala24-Ala92
Accession # Q5QNW0

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse CCL3/MIP‑1 alpha (Catalog # 450-MA)

Mouse CCL3/MIP-1 alpha Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.1-0.4 µg/mL

Use in combination with:

Capture Reagent: Mouse CCL3/MIP‑1 alpha Antibody (Catalog # AF-450-NA)

Standard: Recombinant Mouse CCL3/MIP-1 alpha Protein (Catalog # 450-MA)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL3/MIP-1 alpha

The macrophage inflammatory proteins 1 alpha and 1 beta, two closely related but distinct proteins, were originally co-purified from medium conditioned by a LPS-stimulated murine macrophage cell line. Mature mouse MIP-1 alpha shares approximately 77% and 70% amino acid identity with human MIP-1 alpha and mouse MIP-1 beta, respectively. MIP-1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation. The MIP-1 proteins are members of the beta (C-C) subfamily of chemokines.

Both MIP-1 alpha and MIP-1 beta are monocyte chemoattractants in vitro. Additionally, the MIP-1 proteins have been reported to have chemoattractant and adhesive effects on lymphocytes, with MIP-1 alpha and MIP-1 beta preferentially attracting CD8⁺ and CD4⁺ T cells, respectively. MIP-1 alpha has also been shown to attract B cells as well as eosinophils. MIP-1 proteins have been reported to have multiple effects on hematopoietic precursor cells and MIP-1 alpha has been identified as a stem cell inhibitory factor that can inhibit the proliferation of hematopoietic stem cells in vitro as well as in vivo. In the same assays, MIP-1 beta was reported to be much less active. The functional receptor for MIP-1 alpha has been identified as CCR1 and CCR5.

References

Menten, P. et al. (2002) Cytokine Growth Factor Rev. 13:455.

Entrez Gene IDs

6348 (Human); 20302 (Mouse); 25542 (Rat); 448787 (Canine); 102136134 (Cynomolgus Monkey)

Alternate Names

C-C motif chemokine 3; MIP1-(a); AI323804; CCL3; chemokine (C-C motif) ligand 3; G0S19-1; LD78a; LD78alpha; MIP1 alpha; MIP-1 alpha; MIP1A; MIP-1alpha; MIP1-alpha; PAT 464.1; SCYA3; SIS-beta

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse CCL3/MIP-1 alpha Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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Increased generation of HIV-1 gp120-reactive CD8+ T cells by a DNA vaccine construct encoding the chemokine CCL3.
Authors: Oynebraten I, Hinkula J, Fredriksen A, Bogen B
PLoS ONE, 2014-08-14;9(8):e104814.
Lactobacillus plantarum displaying CCL3 chemokine in fusion with HIV-1 Gag derived antigen causes increased recruitment of T cells
Authors: Katarzyna Kuczkowska, Geir Mathiesen, Vincent G. H. Eijsink, Inger Øynebråten
Microbial Cell Factories
Response patterns of cytokines/chemokines in two murine strains after irradiation.
Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
Cytokine, 2012-01-25;58(2):169-77.
Species: Mouse
Sample Types: Plasma
Applications: Luminex Development
Diesel exhaust particulates exacerbate asthma-like inflammation by increasing CXC chemokines.
Authors: Kim J, Natarajan S, Vaickus LJ, Bouchard JC, Beal D, Cruikshank WW, Remick DG
Am. J. Pathol., 2011-10-01;179(6):2730-9.
Species: Mouse
Sample Types: BALF
Applications: ELISA Development
Resistance of human alveolar macrophages to Bacillus anthracis lethal toxin.
Authors: Wu W, Mehta H, Chakrabarty K, Booth JL, Duggan ES, Patel KB, Ballard JD, Coggeshall KM, Metcalf JP
J. Immunol., 2009-10-07;183(9):5799-806.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA Development
Increased cytokine production in IL-18 receptor alpha-deficient cells is associated with dysregulation of suppressors of cytokine signaling (SOCS).
Authors: Nold-Petry CA, Nold MF, Nielsen JW, Bustamante A, Zepp JA, Storm KA, Hong JW, Kim SH, Dinarello CA
J. Biol. Chem., 2009-07-10;0(0):.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA Development
Neutrophil recruitment in immunized mice depends on MIP-2 inducing the sequential release of MIP-1alpha, TNF-alpha and LTB(4).
Authors: Ramos CD, Fernandes KS, Canetti C, Teixeira MM, Silva JS, Cunha FQ
Eur. J. Immunol., 2006-08-01;36(8):2025-34.
Species: Mouse
Sample Types: Tissue Secretion
Applications: ELISA Development
Selective macrophage suppression during sepsis.
Authors: Ellaban E, Bolgos G, Remick D
Cell. Immunol., 2005-02-26;231(1):103-11.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA Development
Synthesis of several chemokines but few cytokines by primed uncommitted precursor CD4 T cells suggests that these cells recruit other immune cells without exerting direct effector functions.
Authors: Mosmann T
Eur. J. Immunol., 2004-06-01;34(6):1617-26.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA Development, ELISpot Development
A soluble chemokine-binding protein from vaccinia virus reduces virus virulence and the inflammatory response to infection.
Authors: Reading PC, Symons JA, Smith GL
J. Immunol., 2003-02-01;170(3):1435-42.
Species: Human
Sample Types: BALF
Applications: ELISA Development

Immunostimulatory DNA-based vaccines elicit multifaceted immune responses against HIV at systemic and mucosal sites.
Authors: Horner AA, Datta SK, Takabayashi K, Belyakov IM, Cinman N, Nguyen MD, Van Uden JH, Berzofsky JA
J. Immunol., 2001-08-01;167(3):1584-91.
Species: Mouse
Sample Types: Whole Cells
Applications: ELISpot Development

Regulatory effects of eotaxin on acute lung inflammatory injury.
Authors: Guo RF, Lentsch AB, Warner RL, Huber-Lang M, Sarma JV, Hlaing T, Shi MM, Lukacs NW, Ward PA
J. Immunol., 2001-04-15;166(8):5208-18.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA Development