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Mouse CCL4/MIP-1 beta Quantikine ELISA Kit

Catalog #: MMB00
32 Citations 1 Review Datasheet / COA / SDS
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MMB00
Mouse CCL4/MIP-1 beta ELISA Standard Curve
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Citations (32)
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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Mouse CCL4/MIP-1 beta Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (25 uL), EDTA Plasma (25 uL)
Sensitivity
3 pg/mL
Assay Range
7.8 - 500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma)
Specificity
Natural and recombinant mouse MIP-1 beta
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Mouse MIP-1 beta Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse MIP-1 beta levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse MIP-1 beta and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant mouse MIP-1 beta accurately. Results obtained using natural mouse MIP-1 beta showed dose response curves that were parallel to the standard curves obtained using the recombinant standards. These results indicate that this kit can be used to determine relative mass values for natural mouse MIP-1 beta.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 23.1 73 234 23.7 77 245
Standard Deviation 2.2 4 5.4 1.7 3.4 12.5
CV% 9.5 5.5 2.3 7.2 4.4 5.1

Recovery

The recovery of mouse MIP-1 beta spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=5) 104 86-118
EDTA Plasma (n=5) 99 87-106
Serum (n=5) 101 93-109

Linearity

To assess the linearity of the assay, five or more samples containing and/or spiked with various concentrations of mouse MIP-1 beta in each matrix were diluted with Calibrator Diluent and then assayed.
Mouse CCL4/MIP-1 beta ELISA Linearity

Scientific Data

N/A Mouse CCL4/MIP-1 beta ELISA Standard Curve View Larger

Mouse CCL4/MIP-1 beta ELISA Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CCL4/MIP-1 beta

CCL4/MIP-1 beta is a beta chemokine that is secreted at sites of inflammation by activated leukocytes, lymphocytes, vascular endothelial cells, and pulmonary smooth muscle cells. It attracts a variety of immune cells to sites of microbial infection as well as to other pathologic inflammation such as allergic asthma and ischemic myocardium. CCL4 is secreted from activated monocytes as a heterodimer with CCL3/MIP-1 alpha. It signals through CCR5, and an N-terminally trimmed form additionally interacts with CCR1 and CCR2. In humans, the ability of CCL4 to bind CCR5 inhibits the cellular entry of M-tropic HIV-1 which utilizes CCR5 as a coreceptor.

Entrez Gene IDs:
6351 (Human); 20303 (Mouse); 116637 (Rat); 448786 (Canine); 102117861 (Cynomolgus Monkey)
Alternate Names:
ACT2; ACT-2; AT744.1; C-C motif chemokine 4; CCL4; chemokine (C-C motif) ligand 4; Exodus-3; G-26 T-lymphocyte-secreted protein; G-26; HC21; LAG1; LAG-1; Lymphocyte activation gene 1 protein; Macrophage inflammatory protein 1-beta; MIP1 beta; MIP-1 beta; MIP1B; MIP1B1; MIP-1-beta; MIP-1-beta(1-69); PAT 744; Protein H400; SCYA2; SCYA4; secreted protein G-26; SIS-gamma; small inducible cytokine A4 (homologous to mouse Mip-1b); Small-inducible cytokine A4; T-cell activation protein 2
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 50 µL Assay Diluent
  3.   Add 50 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  5.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.
    6. 100 µL Conjugate
  6.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate and wash 5 times.
    8. 100 µL Substrate Solution
  8.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
    9. 100 µL Stop Solution
  9.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Mouse CCL4/MIP-1 beta Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

32 Citations: Showing 1 - 10
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  1. LHPP-mediated inorganic pyrophosphate hydrolysis-driven lysosomal acidification in astrocytes regulates adult neurogenesis
    Authors: Sha, L;Li, J;Shen, H;Wang, Q;Meng, P;Zhang, X;Deng, Y;Zhu, W;Xu, Q;
    Cell reports
    Species: Mouse
    Sample Types: Cell Culture Supernates
  2. Asthma reduces glioma formation by T cell decorin-mediated inhibition of microglia
    Authors: J Chatterjee, S Sanapala, O Cobb, A Bewley, AK Goldstein, E Cordell, X Ge, JR Garbow, MJ Holtzman, DH Gutmann
    Nature Communications, 2021-12-08;12(1):7122.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  3. Lack of whey acidic protein four disulphide core (WFDC) 2 protease inhibitor causes neonatal death from respiratory failure in mice
    Authors: K Nakajima, M Ono, U Radovi?, S Dizdarevi?, SI Tomizawa, K Kuroha, G Naganatsu, I Hoshi, R Matsunaga, T Shirakawa, T Kurosawa, Y Miyazaki, M Seki, Y Suzuki, H Koseki, M Nakamura, T Suda, K Ohbo
    Dis Model Mech, 2019-11-12;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  4. Type I interferon-dependent CCL4 is induced by a cGAS/STING pathway that bypasses viral inhibition and protects infected tissue, independent of viral burden
    Authors: NJ Parekh, TE Krouse, IE Reider, RP Hobbs, BM Ward, CC Norbury
    PLoS Pathog., 2019-10-11;15(10):e1007778.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  5. Tofacitinib enhances delivery of antibody-based therapeutics to tumor cells through modulation of inflammatory cells
    Authors: N Simon, A Antignani, SM Hewitt, M Gadina, C Alewine, D FitzGerald
    JCI Insight, 2019-03-07;0(0):.
    Species: Mouse
    Sample Types: Serum
  6. CCL3 augments tumor rejection and enhances CD8+T cell infiltration through NK and CD103+dendritic cell recruitment via IFN?
    Authors: F Allen, ID Bobanga, P Rauhe, D Barkauskas, N Teich, C Tong, J Myers, AY Huang
    Oncoimmunology, 2017-11-20;7(3):e1393598.
    Species: Mouse
    Sample Types: Serum
    Applications: ELISA Capture
  7. PD-1 blockade enhances elotuzumab efficacy in mouse tumor models
    Authors: NA Bezman, A Jhatakia, AY Kearney, T Brender, M Maurer, K Henning, MR Jenkins, AJ Rogers, PJ Neeson, AJ Korman, MD Robbins, RF Graziano
    Blood Adv, 2017-05-08;1(12):753-765.
    Species: Mouse
    Sample Types: Tissue Homogenates
  8. Behavioural traits propagate across generations via segregated iterative-somatic and gametic epigenetic mechanisms
    Nat Commun, 2016-05-13;7(0):11492.
    Species: Mouse
    Sample Types: Plasma
  9. CCL3 production by microglial cells modulates disease severity in murine models of retinal degeneration.
    Authors: Kohno H, Maeda T, Perusek L, Pearlman E, Maeda A
    J Immunol, 2014-03-17;192(8):3816-27.
    Species: Mouse
    Sample Types: Tissue Homogenates
  10. Cleavage of the T cell protein tyrosine phosphatase by the hepatitis C virus nonstructural 3/4A protease induces a Th1 to Th2 shift reversible by ribavirin therapy.
    Authors: Brenndorfer E, Brass A, Karthe J, Ahlen G, Bode J, Sallberg M
    J Immunol, 2014-01-17;192(4):1671-80.
    Species: Mouse
    Sample Types: Cell Lysates
  11. Interferon gamma-induced intratumoral expression of CXCL9 alters the local distribution of T cells following immunotherapy with
    Authors: Guirnalda P, Wood L, Goenka R, Crespo J, Paterson Y
    Oncoimmunology, 2013-07-23;2(8):e25752.
    Species: Mouse
    Sample Types: Tissue Homogenates
  12. Potential roles of CCR5(+) CCR6(+) dendritic cells induced by nasal ovalbumin plus Flt3 ligand expressing adenovirus for mucosal IgA responses.
    Authors: Fukuyama Y, Tokuhara D, Sekine S, Aso K, Kataoka K, Davydova J, Yamamoto M, Gilbert R, Tokuhara Y, Fujihashi K, Kunisawa J, Yuki Y, Kiyono H, McGhee J, Fujihashi K
    PLoS ONE, 2013-04-02;8(4):e60453.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  13. Modelling of mouse experimental colitis by global property screens: a holistic approach to assess drug effects in inflammatory bowel disease.
    Authors: Gottfries J, Melgar S, Michaelsson E
    PLoS ONE, 2012-01-18;7(1):e30005.
    Species: Mouse
    Sample Types: Tissue Homogenates
  14. CCR5 is involved in resolution of inflammation in proteoglycan-induced arthritis.
    Authors: Doodes PD, Cao Y, Hamel KM, Wang Y, Rodeghero RL, Kobezda T, Finnegan A
    Arthritis Rheum., 2009-10-01;60(10):2945-53.
    Species: Mouse
    Sample Types: Serum
  15. Endotoxin augmented antigen-induced Th1 cell trafficking amplifies airway neutrophilic inflammation.
    Authors: Mikhak Z, Farsidjani A, Luster AD
    J. Immunol., 2009-06-15;182(12):7946-56.
    Species: Mouse
    Sample Types: BALF
  16. TLR9 agonist, but not TLR7/8, functions as an adjuvant to diminish FI-RSV vaccine-enhanced disease, while either agonist used as therapy during primary RSV infection increases disease severity.
    Authors: Johnson TR, Rao S, Seder RA, Chen M, Graham BS
    Vaccine, 2009-04-03;27(23):3045-52.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  17. Role of the chemokine decoy receptor D6 in balancing inflammation, immune activation, and antimicrobial resistance in Mycobacterium tuberculosis infection.
    Authors: Di Liberto D, Locati M, Caccamo N, Vecchi A, Meraviglia S, Salerno A, Sireci G, Nebuloni M, Caceres N, Cardona PJ, Dieli F, Mantovani A
    J. Exp. Med., 2008-08-11;205(9):2075-84.
    Species: Mouse
    Sample Types: Serum
  18. Pulmonary eosinophilia requires interleukin-5, eotaxin-1, and CD4+ T cells in mice immunized with respiratory syncytial virus G glycoprotein.
    Authors: Johnson TR, Rothenberg ME, Graham BS
    J. Leukoc. Biol., 2008-06-02;84(3):748-59.
    Species: Mouse
    Sample Types: Tissue Homogenates
  19. The direct action of 1alpha,25(OH)2-vitamin D3 on purified mouse Langerhans cells.
    Authors: Fujita H, Asahina A, Komine M, Tamaki K
    Cell. Immunol., 2007-05-15;245(2):70-9.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  20. CCL4 protects from type 1 diabetes by altering islet beta-cell-targeted inflammatory responses.
    Authors: Meagher C, Arreaza G, Peters A, Strathdee CA, Gilbert PA, Mi QS, Santamaria P, Dekaban GA, Delovitch TL
    Diabetes, 2007-03-01;56(3):809-17.
    Species: Mouse
    Sample Types: Serum
  21. Cannabinoid-2 receptor mediates protection against hepatic ischemia/reperfusion injury.
    Authors: Batkai S, Osei-Hyiaman D, Pan H, El-Assal O, Rajesh M, Mukhopadhyay P, Hong F, Harvey-White J, Jafri A, Hasko G, Huffman JW, Gao B, Kunos G, Pacher P
    FASEB J., 2007-02-27;21(8):1788-800.
    Species: Mouse
    Sample Types: Tissue Homogenates
  22. Deficiency of P-selectin or P-selectin glycoprotein ligand-1 leads to accelerated development of glomerulonephritis and increased expression of CC chemokine ligand 2 in lupus-prone mice.
    Authors: He X, Schoeb TR, Panoskaltsis-Mortari A, Zinn KR, Kesterson RA, Zhang J, Samuel S, Hicks MJ, Hickey MJ, Bullard DC
    J. Immunol., 2006-12-15;177(12):8748-56.
    Species: Mouse
    Sample Types: Tissue Homogenates
  23. Local production of chemokines and prostaglandin E2 in the acute, chronic and recovery phase of murine experimental colitis.
    Authors: Drmotova M, Rehnstrom E, Jansson L
    Cytokine, 2006-11-07;35(5):275-83.
    Species: Mouse
    Sample Types: Tissue Homogenates
  24. Organ-specific role of MyD88 for gene regulation during polymicrobial peritonitis.
    Authors: Weighardt H, Mages J, Jusek G, Kaiser-Moore S, Lang R, Holzmann B
    Infect. Immun., 2006-06-01;74(6):3618-32.
    Species: Mouse
    Sample Types: Tissue Homogenates
  25. Immunosuppressant triptolide inhibits dendritic cell-mediated chemoattraction of neutrophils and T cells through inhibiting Stat3 phosphorylation and NF-kappaB activation.
    Authors: Liu Q, Chen T, Chen G, Li N, Wang J, Ma P, Cao X
    Biochem. Biophys. Res. Commun., 2006-05-12;345(3):1122-30.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  26. Immunization with gp120-depleted whole killed HIV immunogen and a second-generation CpG DNA elicits strong HIV-specific responses in mice.
    Authors: Trabattoni D, Clivio A, Bray DH, Bhagat L, Beltrami S, Maffeis G, Cesana E, Lowry P, Lissoni F, Kandimalla ER, Sullivan T, Agrawal S, Bartholomew R, Clerici M
    Vaccine, 2005-09-22;24(9):1470-7.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  27. Vbeta14(+) T cells mediate the vaccine-enhanced disease induced by immunization with respiratory syncytial virus (RSV) G glycoprotein but not with formalin-inactivated RSV.
    Authors: Johnson TR, Varga SM, Braciale TJ, Graham BS
    J. Virol., 2004-08-01;78(16):8753-60.
    Species: Mouse
    Sample Types: Tissue Homogenates
  28. The role of gammadelta T cells in the regulation of neutrophil-mediated tissue damage after thermal injury.
    Authors: Toth B, Alexander M, Daniel T, Hubbard WJ, Schwacha MG
    J. Leukoc. Biol., 2004-06-14;76(3):545-52.
    Species: Mouse
    Sample Types: Plasma
  29. Effects of poly(ADP-ribose) polymerase inhibition on inflammatory cell migration in a murine model of asthma.
    Authors: Virag L, Bai P, Bak I, Pacher P, Mabley JG, Liaudet L, Bakondi E, Gergely P, Kollai M, Szabo C
    Med. Sci. Monit., 2004-03-01;10(3):BR77-83.
    Species: Mouse
    Sample Types: BALF
  30. Role of the autocrine chemokines MIP-1alpha and MIP-1beta in the metastatic behavior of murine T cell lymphoma.
    Authors: Menten P, Saccani A, Dillen C, Wuyts A, Struyf S, Proost P, Mantovani A, Wang JM, Van Damme J
    J. Leukoc. Biol., 2002-10-01;72(4):780-9.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  31. Immunostimulatory DNA-based vaccines elicit multifaceted immune responses against HIV at systemic and mucosal sites.
    Authors: Horner AA, Datta SK, Takabayashi K, Belyakov IM, Cinman N, Nguyen MD, Van Uden JH, Berzofsky JA
    J. Immunol., 2001-08-01;167(3):1584-91.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  32. Inhibition of lipopolysaccharide-induced signal transduction in endotoxin-tolerized mouse macrophages: dysregulation of cytokine, chemokine, and toll-like receptor 2 and 4 gene expression.
    Authors: Medvedev AE, Kopydlowski KM, Vogel SN
    J. Immunol., 2000-06-01;164(11):5564-74.
    Species: Mouse
    Sample Types: Cell Culture Supernates

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Mouse CCL4/MIP-1 beta Quantikine ELISA Kit
By Anonymous on 10/26/2022
Sample Tested: Cell culture supernatant

Cell culture supernatants produced from transfected BHK-21 cells

Mouse CCL4/MIP-1 beta Quantikine ELISA Kit MMB00