Mouse CCL6/C10 Biotinylated Antibody

Catalog #: BAM4872 Datasheet / COA / SDS

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BAM4872

All CCL6/C10 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CCL6/C10 Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CCL6/C10 in ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant mouse (rm) CCL3, rmCCL4, or rmCCL9/10 is observed.

Source

Monoclonal Rat IgG_2A Clone # 262008

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant mouse CCL6/C10
Gly22-Ala116
Accession # P27784

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Mouse CCL6/C10 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.5-2 µg/mL

Use in combination with:

Capture Reagent: Mouse CCL6/C10 Antibody (Catalog # MAB4871)

Standard: Recombinant Mouse CCL6/C10 Protein (Catalog # 487-C)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL6/C10

Mouse CCL6, a member of the beta subfamily of chemokines, was initially identified as a transcript that is induced in bone marrow cells upon stimulation with GM-CSF. A human homologue for mouse CCL6 has not been identified. The mCCL6 cDNA encodes a 116 amino acid precursor protein with a hydrophobic signal peptide that is cleaved to yield a 95 amino acid mature protein. Compared to other chemokines, mCCL6 has a large N-terminal extension. The expression of CCL6 mRNA has been detected in mouse monocytes and neutrophils, where it is strongly induced upon GM-CSF stimulation. CCL6 expression has also been detected in an IL-2-dependent T cell line, where the expression is inhibited following T cell stimulation. Mouse CCL6 expression was also shown to be strongly induced by IL-4 in mouse macrophages. The gene for CCL6 has been mapped to mouse chromosome 11.

Entrez Gene IDs

20305 (Mouse); 287910 (Rat)

Alternate Names

C10; c10;C-C motif chemokine 6;MRP;MRP-1;Scy;Scya6; CCL6; MRP-1; SCYA6