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Mouse CCL7/MCP-3/MARC Antibody

Catalog #: AF-456-NA
15 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF-456-NA
AF-456-SP
Chemotaxis Induced by CCL7/MARC and Neutralization by Mouse CCL7/MARC Antibody.
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Citations (15)
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Mouse CCL7/MCP-3/MARC Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CCL7/MCP-3/MARC in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross‑reactivity with recombinant human Eotaxin and recombinant mouse (rm) JE is observed and less than 5% cross-reactivity with rmMCP-5 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse CCL7/MCP-3/MARC
Gln24-Pro97
Accession # Q03366
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse CCL7/MCP‑3/MARC (Catalog # 456-MC)
Neutralization
Measured by its ability to neutralize CCL7/MCP‑3/MARC-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR2A. The Neutralization Dose (ND50) is typically 7.5-45 µg/mL in the presence of 2 µg/mL Recombinant Mouse CCL7/MCP‑3/MARC.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Chemotaxis Induced by CCL7/MARC and Neutralization by Mouse CCL7/MARC Antibody. View Larger

Chemotaxis Induced by CCL7/MARC and Neutralization by Mouse CCL7/MARC Antibody. Recombinant Mouse CCL7/ MARC (Catalog # 456-MC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL7/ MARC (2 µg/mL) is neutralized (green line) by increasing concentrations of Mouse CCL7/MARC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-456-NA). The ND50 is typically 7.5-45 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL7/MCP-3/MARC

Mouse MARC, a member of the beta  subfamily of chemokines, was initially identified as a transcript that is induced in a mouse mast cell line after Fc epsilon RI triggering by IgE plus antigen. Sequence comparisons suggest that MARC may be the mouse homologue of the human MCP-3 gene. Mouse MARC/MCP-3 expression has also been detected during murine experimental allergic encephalomyelitis in the spinal cord, and in LPS-stimulated murine WEHI -3 cells and Swiss 3T3 cells where MARC expression is glucocorticoid-attenuated. Except for one amino acid subsititution, mouse MARC is identical to mouse FIC, the product of a growth factor‑activated gene. The mouse MARC cDNA encodes a 97 amino acid residue precursor protein with a 23 amino acid residue signal peptide that is cleaved to yield a 74 amino acid residue mature protein. Mouse CCR2, a mouse chemokine receptor, has been shown to bind JE/MCP-1 with high affinity and MARC/MCP-3 with lower affinity. The E. coli-expressed mouse MARC/MCP-3 produced at R&D Systems has been shown to be a monocyte and T-lymphocyte chemoattractant.

References
  1. Kulmburg, P.A. et al. (1992) J. Exp. Med. 176:1773.
  2. Thirion, S. et al. (1994) Biochem. Biophys. Res. Commun. 201:493.
  3. Smith, J.B. and H.R. Herschman (1995) J. Biol. Chem. 270:16756.
  4. Kurihara, T. and R. Bravo (1996) J. Biol. Chem. 271:11603.
Entrez Gene IDs
6354 (Human); 20306 (Mouse)
Alternate Names
C-C motif chemokine 7; CCL7; chemokine (C-C motif) ligand 7; FIC; MARC; MCP-3; MCP3Monocyte chemotactic protein 3; MCP-3Small-inducible cytokine A7; MGC138465; Monocyte chemoattractant protein 3; NC28SCYA6; SCYA7MGC138463; small inducible cytokine A7 (monocyte chemotactic protein 3)

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Citations for Mouse CCL7/MCP-3/MARC Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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  1. Hypoxia inducible factor 1 alpha in vascular smooth muscle cells promotes angiotensin II-induced vascular remodeling via activation of CCL7-mediated macrophage recruitment
    Authors: Qi D, Wei M, Jiao S et al.
    Cell Death Dis
  2. Glioma-derived CCL2 and CCL7 mediate migration of immune suppressive CCR2+/CX3CR1+ M-MDSCs into the tumor microenvironment in a redundant manner
    Authors: Gregory P. Takacs, Christian J. Kreiger, Defang Luo, Guimei Tian, Julia S. Garcia, Loic P. Deleyrolle et al.
    Frontiers in Immunology
  3. Essential roles of the cytokine oncostatin M in crosstalk between muscle fibers and immune cells in skeletal muscle after aerobic exercise
    Authors: T Komori, Y Morikawa
    The Journal of Biological Chemistry, 2022-11-09;0(0):102686.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  4. ROS-induced PADI2 downregulation accelerates cellular senescence via the stimulation of SASP production and NF kappa B activation
    Authors: Hyun-Jung Kim, Woo-Jin Kim, Hye-Rim Shin, Hee-In Yoon, Jae-I Moon, Eunji Lee et al.
    Cellular and Molecular Life Sciences
  5. CCL7 contributes to angiotensin II-induced abdominal aortic aneurysm by promoting macrophage infiltration and pro-inflammatory phenotype
    Authors: C Xie, F Ye, N Zhang, Y Huang, Y Pan, X Xie
    Journal of Cellular and Molecular Medicine, 2021-06-29;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  6. Deficiency in CCR2 increases susceptibility of mice to infection with an intracellular pathogen, Francisella tularensis LVS, but does not impair development of protective immunity
    Authors: SL Kurtz, R De Pascali, AI Meierovics, KL Elkins
    PLoS ONE, 2021-03-24;16(3):e0249142.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  7. Reticular Fibroblasts Expressing the Transcription Factor WT1 Define a Stromal Niche that Maintains and Replenishes Splenic Red Pulp Macrophages
    Authors: Alicia Bellomo, Isabelle Mondor, Lionel Spinelli, Marine Lagueyrie, Benjamin J. Stewart, Nicolas Brouilly et al.
    Immunity
  8. Fbxw7 increases CCL2/7 in CX3CR1hi macrophages to promote intestinal inflammation
    Authors: J He, Y Song, G Li, P Xiao, Y Liu, Y Xue, Q Cao, X Tu, T Pan, Z Jiang, X Cao, L Lai, Q Wang
    J. Clin. Invest., 2019-06-27;130(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Chemokines CCL2 and CCL7, but not CCL12, play a significant role in the development of pain-related behavior and opioid-induced analgesia
    Authors: K Kwiatkowsk, K Popiolek-B, A Piotrowska, E Rojewska, K Ciapa?a, W Makuch, J Mika
    Cytokine, 2019-04-16;119(0):202-213.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  10. CCL7 and IRF-7 Mediate Hallmark Inflammatory and IFN Responses following Rhinovirus 1B Infection.
    Authors: Girkin J, Hatchwell L, Foster P, Johnston S, Bartlett N, Collison A, Mattes J
    J Immunol, 2015-04-06;194(10):4924-30.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  11. The expression of Fn14 via mechanical stress-activated JNK contributes to apoptosis induction in osteoblasts.
    Authors: Matsui H, Fukuno N, Kanda Y, Kantoh Y, Chida T, Nagaura Y, Suzuki O, Nishitoh H, Takeda K, Ichijo H, Sawada Y, Sasaki K, Kobayashi T, Tamura S
    2014-01-20;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  12. B lymphocytes trigger monocyte mobilization and impair heart function after acute myocardial infarction
    Authors: Yasmine Zouggari, Hafid Ait-Oufella, Philippe Bonnin, Tabassome Simon, Andrew P Sage, Coralie Guérin et al.
    Nature Medicine
  13. Identification of Chemoattractive Factors Involved in the Migration of Bone Marrow-Derived Mesenchymal Stem Cells to Brain Lesions Caused by Prions
    Authors: Chang-Hyun Song, Osamu Honmou, Hidefumi Furuoka, Motohiro Horiuchi
    Journal of Virology
  14. Comprehensive assessment of chemokine expression profiles by flow cytometry.
    Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
    J. Clin. Invest., 2010-02-08;120(3):907-23.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  15. The CCL7-CCL2-CCR2 axis regulates IL-4 production in lungs and fungal immunity.
    Authors: Szymczak WA, Deepe GS
    J. Immunol., 2009-07-08;183(3):1964-74.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization

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