Mouse CCR1 Antibody

Catalog #: MAB5986
5 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5986
MAB5986-SP

Detection of Mouse CCR1 by Western Blot.

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All CCR1 Antibodies

Product Details

Citations (5)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CCR1 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CCR1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse CCR4, 5, or 8 is observed.

Source

Monoclonal Rat IgG_2B Clone # 643854

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant mouse CCR1
Extracellular regions (aa 1-34, aa 92-107, aa 172-192, aa 265-281)
Accession # P51675

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Flow Cytometry

2.5 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse CCR1 by Western Blot. Western blot shows lysates of mouse heart tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse CCR1 Monoclonal Antibody (Catalog # MAB5986) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for CCR1 at approximately 43 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry

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Detection of CCR1 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse CCR1 Monoclonal Antibody (Catalog # MAB5986, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCR1

CCR1 (C-C chemokine receptor 1; also MIP-1a receptor and CD191) is a 43 kDa member of the GPCR #1 family of transmembrane proteins. Mouse CCR1 is expressed on osteoclasts, IL-13⁺ T cells, neutrophils, bone marrow-derived mast cells, eosinophils, monocytes and vascular intimal smooth muscle cells. Multiple chemokines are reported to bind to CCR1. MIP-1a/CCL3 and RANTES/CCL5 are natural ligands, while CCL15, CCL9 and CCL23 are potent angonists after N-terminal processing. Mouse CCR1 is a 7-transmembrane protein that is 355 amino acids (aa) in length. It contains a 34 aa N-terminal extracellular domain plus a
50 aa C-terminal cytoplasmic tail. Over aa sequences 1-34, 92-107, 172-192 and 265-281 collectively, mouse CCR1 shares 76% and 89% aa identity with human and rat CCR1, respectively.

Entrez Gene IDs

1230 (Human); 12768 (Mouse); 57301 (Rat)

Alternate Names

C-C chemokine receptor type 1; CC-CKR-1; CCR1; CCR-1; CD191 antigen; CD191; chemokine (C-C motif) receptor 1; CKR1; CKR-1; CMKBR1SCYAR1; CMKR1; HM145C-C CKR-1; LD78 receptor; Macrophage inflammatory protein 1-alpha receptor; MIP-1alpha-R; MIP1aR; RANTES receptor; RANTES-R

Product Datasheets

Product Datasheet

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Related Research Areas

Citations for Mouse CCR1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Luteolin Alleviates Liver Fibrosis in Rat Hepatic Stellate Cell HSC-T6: A Proteomic Analysis
Authors: Batudeligen, ;Han, Z;Chen, H;Narisu, ;Xu, Y;Anda, ;Han, G;
Drug design, development and therapy
Species: Rat
Sample Types: Cell Lysates
Applications: Western Blot
CCL7 contributes to angiotensin II-induced abdominal aortic aneurysm by promoting macrophage infiltration and pro-inflammatory phenotype
Authors: C Xie, F Ye, N Zhang, Y Huang, Y Pan, X Xie
Journal of Cellular and Molecular Medicine, 2021-06-29;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot
The role of CCR1 and therapeutic effects of anti‐CCL3 antibody in herpes simplex virus‐induced Behçet's disease mouse model
Authors: Hasan M. Sayeed, Eun‐So Lee, Hae‐Ok Byun, Seonghyang Sohn
Immunology
CCR1 plays a critical role in modulating pain through hematopoietic and non-hematopoietic cells.
Authors: Lewis, Nuruddee, Muthukumarana, Akalushi, Fogal, Steven E, Corradini, Laura, Stefanopoulos, Dimitria, Adusumalli, Prathima, Pelletier, Josephin, Panzenbeck, Mark, Berg, Karen, Canfield, Melissa, Cook, Brian N, Razavi, Hossein, Kuzmich, Daniel, Anderson, Shawn, Allard, Devan, Harrison, Paul, Grimaldi, Christin, Souza, Donald, Harcken, Christia, Fryer, Ryan M, Modis, Louise K, Brown, Maryanne
PLoS ONE, 2014-08-29;9(8):e105883.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
MIP-1alpha/CCL3-mediated maintenance of leukemia-initiating cells in the initiation process of chronic myeloid leukemia.
Authors: Baba T, Naka K, Morishita S, Komatsu N, Hirao A, Mukaida N
J Exp Med, 2013-10-28;210(12):2661-73.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry