Mouse CCR10 PerCP-conjugated Antibody

Catalog #: FAB2815C Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB2815C-025
FAB2815C-100

Detection of CCR10 in B16‑F1 Mouse Cell Line by Flow Cytometry.

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Mouse CCR10 PerCP-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CCR10. Stains mouse CCR10-transfected cells but not irrelevant transfectants.

Source

Monoclonal Rat IgG_2A Clone # 248918

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

C6 rat glioma cell line transfected with mouse CCR10
Met1-Asn362
Accession # NP_031747

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

PerCP (Peridinin-chlorophyll Protein Complex) (Excitation= 482 and 564 nm, Emission= 675 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CCR10 in B16‑F1 Mouse Cell Line by Flow Cytometry. B16-F1 mouse melanoma cell line was stained with Rat Anti-Mouse CCR10 PerCP-conjugated Monoclonal Antibody (Catalog # FAB2815C, filled histogram) or isotype control antibody (Catalog # IC006C, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry

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Detection of CCR10 in BaF3 Mouse Cell Line Transfected with CCR10 and eGFP by Flow Cytometry. BaF3 mouse pro-B cell line transfected with mouse CCR10 and eGFP was stained with Rat Anti-Mouse CCR10 PerCP-conjugated Monoclonal Antibody (Catalog # FAB2815C) and. Quadrant markers were set based on control antibody staining (Catalog # IC006C). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry

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Detection of CCR10 in HEK293 Human Cell Line transfected with Mouse CCR10 and eGFP vs irrelevant GPCR by Flow Cytometry HEK293 Human Cell Line transfected with Mouse CCR10 and eGFP (A) vs irrelevant GPCR (B) were stained with Rat Anti-Mouse CCR10 PerCP‑conjugated Monoclonal Antibody (Catalog # fab2815C) and eGFP isotype control antibody (Catalog # IC006C). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCR10

CCR10 is a G protein-linked seven transmembrane domain protein expressed by T cells and B cell subsets that functions as a receptor for CCL27. CCR10 mediates lymphocyte migration to the skin and mucosa, and its expression correlates with the metastatic capacity of melanomas.

Entrez Gene IDs

2826 (Human); 12777 (Mouse); 363682 (Rat)

Alternate Names

CC chemokine receptor 10; C-C chemokine receptor type 10; C-C CKR-10; CC-CKR-10; CCR10; CCR-10; chemokine (C-C motif) receptor 10; G protein-coupled receptor 2; GPR2; GPR2G-protein coupled receptor 2