Mouse CCRL2/LCCR Antibody

Catalog #: MAB5519
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5519
MAB5519-SP

Detection of CCRL2/L‑CCR in RAW 264.7 Mouse Cell Line by Flow Cytometry.

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All CCRL2/LCCR Antibodies

Product Details

Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CCRL2/LCCR Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CCRL2/LCCR in direct ELISAs.

Source

Monoclonal Rat IgG₁ Clone # 498321

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

RBL-1 rat basophilic leukemia cell line transfected with mouse CCRL2/LCCR
Accession # O35457

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Flow Cytometry

2.5 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CCRL2/L‑CCR in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse CCRL2/L-CCR Monoclonal Antibody (Catalog # MAB5519, filled histogram) or isotype control antibody (Catalog # MAB005, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).

Immunocytochemistry

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CCRL2/L‑CCR/CRAM‑A/B in RAW 264.7 Mouse Cell Line. CCRL2/L-CCR/CRAM-A/B was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line using Rat Anti-Mouse CCRL2/L-CCR/CRAM-A/B Monoclonal Antibody (Catalog # MAB5519) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCRL2/LCCR

Lipopolysaccharide-inducible CC chemokine receptor (LCCR), also known as CCRL2, is a 7TM chemokine receptor-like protein that is expressed on macrophages, glial cells, and mast cells at inflammatory sites. LCCR functions as a receptor for Chemerin, CCL2, 5, 7, and 8. Mouse LCCR shares 49% and 70% aa sequence identity with human and rat LCCR, respectively.

Long Name

CC Chemokine Receptor-like 2

Entrez Gene IDs

54199 (Mouse)

Alternate Names

ACKR5;C-C chemokine receptor-like 2;CKRX;CRAM;CRAM-A;CRAM-B;HCR; CCRL2/LCCR; LCCR

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Mouse CCRL2/LCCR Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Studies with neutralizing antibodies suggest CXCL8-mediated neutrophil activation is independent of C-C motif chemokine receptor-like 2 (CCRL2) ligand binding function
Authors: Z Su, J Brooks, J Pelker, T Andreyeva, H Sobon, R Gifford, M Powers, J Wang, C Dower, M Hegen, D Messing, AD Sheehan, JJ Brennan
PLoS ONE, 2023-01-20;18(1):e0280590.
Species: Human
Sample Types: Transfected Whole Cells
Applications: Flow Cytometry, Neutralization