Mouse CD11b/Integrin alpha M Antibody

Catalog #: MAB11242 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB11242-100
MAB11242-SP

Detection of Mouse CD11b/Integrin alpha M by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CD11b/Integrin alpha M Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CD11b/Integrin alpha M in direct ELISAs.

Source

Recombinant Monoclonal Rabbit IgG Clone # 1250D

Purification

Protein A or G purified from cell culture supernatant

Immunogen

MouseCD11b/Integrin alpha M synthetic peptide
Accession # P11215

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

Mouse brain

Simple Western

5 µg/mL

Mouse brain and rat brain

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse CD11b/Integrin alpha M by Western Blot. Western blot shows lysates of mouse brain. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Mouse CD11b/Integrin alpha M Monoclonal Antibody (Catalog # MAB11242) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for CD11b/Integrin alpha M at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Simple Western

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Detection of Mouse and Rat CD11b/Integrin alpha M by Simple Western^TM. Simple Western lane view shows lysates of mouse brain and rat brain, loaded at 0.2 mg/mL. A specific band was detected for CD11b/Integrin alpha M at approximately 180-200 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Mouse CD11b/Integrin alpha M Monoclonal Antibody (Catalog # MAB11242). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD11b/Integrin alpha M

The Integrin alpha M subunit (CD11b), associates with the Integrin beta 2 subunit (CD18) to form the non-covalent heterodimeric Integrin CD11b/CD18, also known as Mac-1 and CR-3. Upon activation, CD11b/CD18 is expressed on granulocytes, monocytes, a subset of NK cells and activated lymphocytes. Integrin CD11b/CD18 functions as a receptor for complement fragment iC3b, ICAM-1 (CD54), ICAM-2 (CD102) and fibrinogen to mediate phagocyte adhesion, migration and ingestion of complement-opsonized particles (1‑3).

References

Springer, T.A. et al. (1978) Eur. J. Immunol. 8:539.
Springer, T.A. et al. (1979) Eur. J. Immunol. 9:301.
Springer, T.A. et al. (1982) Immunol. Rev. 68:171.

Entrez Gene IDs

3684 (Human); 16409 (Mouse)

Alternate Names

antigen CD11b (p170); CD11 antigen-like family member B; CD11b antigen; CD11b; Cell surface glycoprotein MAC-1 subunit alpha; Complement component 3 receptor 3 subunit; CR-3 alpha chain; CR3A; CR3AMGC117044; Integrin alpha M; integrin alpha-M; integrin, alpha M (complement component 3 receptor 3 subunit); ITGAM; Leukocyte adhesion receptor MO1; MAC-1; MAC1A; macrophage antigen alpha polypeptide; MO1A; neutrophil adherence receptor alpha-M subunit; Neutrophil adherence receptor; SLEB6