Mouse CD161/NK1.1 APC‑conjugated Antibody

Name: Mouse CD161/NK1.1 APC‑conjugated Antibody
Brand: R&D Systems
SKU: FAB8319A

Catalog #: FAB8319A Datasheet / COA / SDS

Discontinued Product

FAB8319A has been discontinued.

View all CD161/NK1.1 products.

Detection of CD161/NK1.1 in C57BL/6 Mouse Splenocytes by Flow Cytometry.

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All CD161/NK1.1 Antibodies

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Mouse CD161/NK1.1 APC‑conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CD161/NK1.1 by flow cytometry

Source

Monoclonal Mouse IgG_2A Clone # PK136

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse NK-1⁺ splenocytes and bone marrow cells

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CD161/NK1.1 in C57BL/6 Mouse Splenocytes by Flow Cytometry. C57BL/6 mouse splenocytes were stained with Rat Anti-Mouse NKp46/NCR1 PE-conjugated Monoclonal Antibody (Catalog # FAB22252P) and either (A) Mouse Anti-Mouse CD161/NK1.1 APC-conjugated Monoclonal Antibody (Catalog # FAB8319A) or (B) Mouse IgG_2AAllophycocyanin Isotype Control (Catalog # IC003A). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD161/NK1.1

CD161, also known as NK1.1, KLRB-1C, Ly-55, and NKR-P1c, is a 28 kDa type 2 transmembrane protein in the Killer Cell Lectin-like Receptor family. CD161 is expressed as a disulfide-linked homodimer on the surface of NK cells and subpopulations of NKT, CD4⁺, CD8⁺, and gamma /δ T cells. Its cross-linking on NK cells induces cytolytic activity, and on CD4 cells it promotes IgE production and the expansion of Th2 responses. Mature mouse CD161 consists of a 45 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 157 aa extracellular domain with one C-type lectin domain.

Entrez Gene IDs

17059 (Mouse)

Alternate Names

CD161 antigen; CD161; CD161/NK1.1; CD161c; CLEC5BC-type lectin domain family 5 member B; HNKR-P1a; killer cell lectin-like receptor subfamily B member 1; killer cell lectin-like receptor subfamily B, member 1; Klrb1c; Ly55c; Ly-59; Natural killer cell surface protein P1A; NKR-P1; NKR-P1AMGC138614; NKRP1ANKR