Mouse CD30/TNFRSF8 Biotinylated Antibody

Catalog #: BAF852
1 Citation Datasheet / COA / SDS

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BAF852

All CD30/TNFRSF8 Antibodies

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Citations (1)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CD30/TNFRSF8 Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CD30/TNFRSF8 in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross‑reactivity with recombinant human (rh) CD30, recombinant mouse (rm) TNF RI, rhTNF RI, rmTNF RII, and rhTNF RII is observed. In Western blots, less than 1% cross-reactivity with rhCD30 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CD30/TNFRSF8
Phe19-Thr281
Accession # Q60846

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse CD30/TNFRSF8 Fc Chimera (Catalog # 852-CD)

Mouse CD30/TNFRSF8 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.1-0.4 µg/mL

Use in combination with:

Capture Reagent: Mouse CD30/TNFRSF8 Antibody (Catalog # MAB8521)

Standard: Recombinant Mouse CD30/TNFRSF8 Fc Chimera Protein, CF (Catalog # 852-CD)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD30/TNFRSF8

CD30, also known as Ki-1 antigen and TNFRSF8, is a 120 kDa type I transmembrane glycoprotein belonging to the TNF receptor superfamily (1, 2). Mature mouse CD30 consists of a 264 amino acid (aa) extracellular domain (ECD) with three cysteine-rich repeats, a 27 aa transmembrane segment, and a 190 aa cytoplasmic domain (3). In contrast, human CD30 includes an additional 90 aa in the ECD and contains six cysteine-rich repeats. Within common regions of the ECD, mouse CD30 shares 53% and 80% aa sequence identity with human and rat CD30, respectively. CD30 is normally expressed on antigen‑stimulated Th cells and B cells (4‑6). However, it is upregulated in Hodgkin’s disease (on Reed-Sternberg cells), other lymphomas, chronic inflammation, and autoimmunity (7). CD30 binds to CD30 Ligand/TNFSF8 which is expressed on activated Th cells, monocytes, granulocytes and medullary thymic epithelial cells (1, 5). CD30 signaling costimulates antigen‑induced Th0 and Th2 proliferation and cytokine secretion but favors a Th2-biased immune response (8). In the absence of antigenic stimulation, it can still induce T cell expression of IL-13 (9). CD30 contributes to thymic negative selection by inducing the apoptotic cell death of CD4⁺CD8⁺ T cells (10, 11). In B cells, CD30 ligation promotes cellular proliferation and antibody production in addition to the expression of CXCR4, CCL3, and CCL5 (5, 12). An 85‑90 kDa soluble form of CD30 is shed from the cell surface by TACE-mediated cleavage (13, 14). Soluble CD30 retains the ability to bind CD30 Ligand and functions as an inhibitor of normal CD30 signaling (15).

References

Kennedy, M.K. et al. (2006) Immunology 118:143.
Tarkowski, M. (2003) Curr. Opin. Hematol. 10:267.
Bowen, M.A. et al. (1996) J. Immunol. 156:442.
Hamann, D. et al. (1996) J. Immunol. 156:1387.
Shanebeck, S.D. et al. (1995) Eur. J. Immunol. 25:2147.
Gruss, H.-J. et al. (1994) Blood 83:2045.
Oflzoglu E. et al. (2009) Adv. Exp. Med. Biol. 647:174.
Del Prete, G. et al. (1995) J. Exp. Med. 182:1655.
Harlin, H. et al. (2002) J. Immunol. 169:2451.
Amakawa, R. et al. (1996) Cell 84:551.
Chiarle, R. et al. (1999) J. Immunol. 163:194.
Vinante, F. et al. (2002) Blood 99:52.
Hansen, H.P. et al. (1995) Int. J. Cancer 63:750.
Hansen, H.P. et al. (2000) J. Immunol. 165:6703.
Hargreaves, P.G. and A. Al-Shamkhani (2002) Eur. J. Immunol. 32:163.

Entrez Gene IDs

943 (Human); 21941 (Mouse); 25069 (Rat)

Alternate Names

CD30 antigen; CD30; CD30KI-1; CD30L receptor; cytokine receptor CD30; D1S166EKi-1; Ki-1 antigen; Lymphocyte activation antigen CD30; TNFRSF8; tumor necrosis factor receptor superfamily member 8; tumor necrosis factor receptor superfamily, member 8

Product Datasheets

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Related Research Areas

Citation for Mouse CD30/TNFRSF8 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Response patterns of cytokines/chemokines in two murine strains after irradiation.
Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
Cytokine, 2012-01-25;58(2):169-77.
Species: Mouse
Sample Types: Plasma
Applications: Luminex Development