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Mouse CD39/ENTPD1 Antibody

Catalog #: MAB4398
1 Citation Datasheet / COA / SDS
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MAB4398
MAB4398-SP
Detection of CD39/ENTPD1 in Mouse Splenocytes by Flow Cytometry.
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Mouse CD39/ENTPD1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CD39/ENTPD1 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human CD39 is observed and no cross-reactivity with recombinant mouse CD39L3 or recombinant human CD39L4 is observed.
Source
Monoclonal Rat IgG1 Clone # 495826
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CD39/ENTPD1
Thr38-Ile478
Accession # AAH11278
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse CD39/ENTPD1 (Catalog # 4398-EN)
Flow Cytometry
2.5 µg/106 cells
See below
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Mouse CD39/ENTPD1 (Catalog # 4398-EN), see our available Western blot detection antibodies
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CD39/ENTPD1 antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of CD39/ENTPD1 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD39/ENTPD1 Monoclonal Antibody (Catalog # MAB4398, filled histogram) or isotype control antibody (Catalog # MAB005, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')2Secondary Antibody (Catalog # F0113).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD39/ENTPD1

Ectonucleoside triphosphate diphosphohydrolase-1 (NTPDase-1) is an integral membrane protein with an extracellular active site. NTPDase-1 was originally described as CD39, a B lymphocyte cell surface marker (1), but it is also present on the surface of natural killer cells, T cells, and some endothelial cells (2). NTPDase-1 hydrolyzes the beta - and gamma  phosphate residues of nucleotides, preferring ATP as the substrate. Through its hydrolysis of extracellular nucleotides, NTPDase-1 plays a role in the regulation of purinergic signaling (3). NTPDase-1 is involved in the processes of thromboregulation and vascular inflammation (4). The administration of soluble NTPDase-1 may have therapeutic applications for the treatment of some vascular and transplantation-associated diseases (5).

References
  1. Rowe, M. et al. (1982) Int. J. Cancer 29:373.
  2. Kansas, G.S. et al. (1991) J. Immunol. 146:2235.
  3. Kishore, B.K. et al. (2005) Am. J. Physiol. Renal Physiol. 288:F1032.
  4. Marcus, A.J. et al. (2005) Semin. Thromb. Hemost. 31:234.
  5. Robson, S.C. et al. (2005) Semin. Thromb. Hemost. 31:217.
Long Name
Ecto-Nucleoside Triphosphate Diphosphohydrolase 1
Entrez Gene IDs
953 (Human); 12495 (Mouse); 64519 (Rat); 102132860 (Cynomolgus Monkey)
Alternate Names
ADPase; ATPDase; ATP-diphosphatase; CD39 antigen; CD39; CD39EC 3.6.1.5; DKFZp686D194; DKFZp686I093; EC 3.6.1; ecto-apyrase; Ecto-ATP diphosphohydrolase 1; Ecto-ATPase 1; Ecto-ATPDase 1; ectonucleoside triphosphate diphosphohydrolase 1; ENTPD1; FLJ40921; FLJ40959; Lymphoid cell activation antigen; NTPD1; NTPDase 1; NTPDase-1

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Citation for Mouse CD39/ENTPD1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Expression and distribution of ectonucleotidases in mouse urinary bladder.
    Authors: Yu W, Robson SC, Hill WG
    PLoS ONE, 2011-04-14;6(4):e18704.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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