Mouse CD39L3/ENTPD3 Antibody

Name: Mouse CD39L3/ENTPD3 Antibody
Brand: R&D Systems
SKU: MAB4464

Catalog #: MAB4464 Datasheet / COA / SDS

Discontinued Product

MAB4464 has been discontinued.

View all CD39L3/ENTPD3 products.

Detection of CD39L3/ENTPD3 in Neuro-2A Mouse Cell Line by Flow Cytometry.

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All CD39L3/ENTPD3 Antibodies

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Mouse CD39L3/ENTPD3 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CD39L3/ENTPD3 in direct ELISAs. In Western blots, less than 15% cross-reactivity with recombinant human (rh) CD39L4 and no cross-reactivity with rhCD39L3, recombinant mouse (rm) CD39, or rmCD39L2 is observed.

Source

Monoclonal Rat IgG₁ Clone # 568610

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse CD39L3/ENTPD3
Gln44-Pro485
Accession # NP_848791

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

2.5 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CD39L3/ENTPD3 in Neuro-2A Mouse Cell Line by Flow Cytometry. Neuro-2A mouse neuroblastoma cell line was stained with Mouse CD39L3/ENTPD3 Monoclonal Antibody (Catalog # MAB4464, filled histogram) or isotype control antibody (Catalog # MAB005, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')₂Secondary Antibody (Catalog # F0105B).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD39L3/ENTPD3

Ectonucleoside triphosphate diphosphohydrolase-3 (NTPDase-3) is an integral membrane protein with an extracellular catalytic domain (1). rhNTPDase-3 was expressed as a protein lacking its N- and C-terminal transmembrane domains, resulting in the secretion of the soluble rhNTPDase-3 ectodomain. NTPDase-3 hydrolyzes the beta - and gamma -phosphate groups of nucleotides, preferring ATP, ADP, UTP, and UDP as substrates (1). Through its hydrolysis of extracellular nuceotides, NTPDase-3 is important for the regulation of purinergic signaling (2). The enzyme is expressed at its highest levels in brain, pancreas, spleen, and prostate tissues (3). In the brain, NTPDase-3 may play a role in the regulation of feeding, sleep, and other behaviors (4).

References

Lavoie, E.G. et al. (2004) Biochem. Pharmacol. 67:1917.
Crawford, P.A. et al. (2007) Arch. Biochem. Biophys. 457:7.
Chadwick, B.P. and A.M. Frischauf (1998) Genomics 50:357.
Belcher, S.M. et al. (2006) Neuroscience 137:1331.

Long Name

Ecto-Nucleoside Triphosphate Diphosphohydrolase 3

Entrez Gene IDs

956 (Human); 215446 (Mouse); 316077 (Rat)

Alternate Names

ATPDase; CD39 antigen-like 3; CD39L3; CD39L3EC 3.6.1.5; CD39-like 3; EC 3.6.1; Ecto-apyrase 3; Ecto-ATP diphosphohydrolase 3; Ecto-ATPase 3; Ecto-ATPDase 3; ectonucleoside triphosphate diphosphohydrolase 3; ENTPD3; FLJ93839; HB6; HB6NTPDase 3; NTPDase-3