Mouse CD40/TNFRSF5 Antibody Summary
Extracellular domain
Applications
The ED50 for this effect is typically 0.03-0.18 μg/mL (1).
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD40/TNFRSF5
CD40 is a type I transmembrane glycoprotein belonging to the TNF receptor superfamily. The mature mCD40 consists of a 172 amino acid (aa) extracellular domain, a 22 aa transmembrane region and a 90 aa cytoplasmic domain (1). CD40 is expressed on B cells, follicular dendritic cells, dendritic cells, activated monocytes, macrophages, endothelial cells, vascular smooth muscle cells and several tumor cell lines (2). The extracellular domain has the cysteine-rich repeat regions, which are characteristic for many of the receptors of the TNF superfamily. Interaction of CD40 with its ligand, CD40L, leads to the aggregation of CD40 molecules, which in turn interact with cytoplasmic components to initiate signaling pathways. Early studies on the CD40-CD40L system revealed its role in humoral immunity. Interaction between CD40L on T cells and CD40 on B cells stimulated B cell proliferation and provided the signal for immunoglobulin isotype switching (3). Mutations in the CD40L gene, which resulted in a CD40L molecule unable to interact with CD40, are responsible for the hyper-IgM syndrome (4). Cross-linking of CD40 with antibodies or by binding to CD40L produces cell type-specific responses which include costimulation and induction of proliferation, induction of cytokine production, rescue from apoptosis, and upregulation of adhesion molecules (5). Some of the early events of intracellular signaling by the CD40-CD40L system include the association of the CD40 with TRAFs and the activation of various kinases (6‑8).
- Torres, R.M. and E.A. Clark (1992) J. Immunol. 148:620.
- Schonbeck, U. et al. (1997) J. Biol. Chem. 272:19569.
- Armitage, R.J. et al. (1993) J. Immunol. 150:3671.
- Callard, R.E. et al. (1993) Immunol. Today 14:559.
- Stout, R.D. and J. Suttles (1996) Immunol. Today 17:487.
- Pullen, S.S. et al. (1999) Biochemistry 38:10168.
- Faris, M. et al. (1994) J. Exp. Med. 179:1923.
- Hanissian, S.H. and R.S. Geha (1997) Immunity 6:379.
Product Datasheets
Citations for Mouse CD40/TNFRSF5 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Inhibition of Rag GTPase signaling in mice suppresses B cell responses and lymphomagenesis with minimal detrimental trade-offs
Authors: A Ortega-Mol, C Lebrero-Fe, A Sanz, N Deleyto-Se, AB Plata-Góme, C Menéndez, O Graña-Cast, E Caleiras, A Efeyan
Cell Reports, 2021-07-13;36(2):109372.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization -
Monoclonal Antibody Targeting Sialyl-di-Lewisa - Containing Internalizing and non-Internalizing Glycoproteins with Cancer Immunotherapy Development Potential
Authors: ST Tivadar, RS McIntosh, JX Chua, R Moss, T Parsons, AM Zaitoun, S Madhusudan, LG Durrant, M Vankemmelb
Mol. Cancer Ther., 2019-12-23;0(0):.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Oncogenic Rag GTPase signalling enhances B cell activation and drives follicular lymphoma sensitive to pharmacological inhibition of mTOR
Authors: Ana Ortega-Molina, Nerea Deleyto-Seldas, Joaquim Carreras, Alba Sanz, Cristina Lebrero-Fernández, Camino Menéndez et al.
Nature Metabolism
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Epitope-dependent mechanisms of CD27 neutralization revealed by X-ray crystallography
Authors: Galina Obmolova, Alexey Teplyakov, Thomas J. Malia, Nicole Wunderler, Deborah Kwok, Linda Barone et al.
Molecular Immunology
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Discovering Molecules That Regulate Efferocytosis Using Primary Human Macrophages and High Content Imaging
Authors: Sandra Santulli-Marotto, Alexis Gervais, Jamie Fisher, Brandy Strake, Carol Anne Ogden, Chelsea Riveley et al.
PLOS ONE
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B cells promote induction of experimental autoimmune encephalomyelitis by facilitating reactivation of T cells in the central nervous system.
Authors: Pierson E, Stromnes I, Goverman J
J Immunol, 2013-12-23;192(3):929-39.
Species: Mouse
Sample Types: Whole Cells
Applications: Functional Assay -
NFATc1 Induction in Peripheral T and B Lymphocytes
Authors: Matthias Hock, Martin Vaeth, Ronald Rudolf, Amiya Kumar Patra, Duong Anh Thuy Pham, Khalid Muhammad et al.
The Journal of Immunology
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B-cell receptor signal strength influences terminal differentiation.
Authors: Lechouane F, Bonaud A, Delpy L, Casola S, Oruc Z, Chemin G, Cogne M, Sirac C
Eur J Immunol, 2013-01-24;43(3):619-28.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Suppressive effect of reactive oxygen species on CD40-induced B cell activation.
Authors: Liu J, Yoshida Y, Yamashita U
FEBS Lett., 2007-09-29;581(26):5043-9.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
3BP2 deficiency impairs the response of B cells, but not T cells, to antigen receptor ligation.
Authors: de la Fuente MA, Kumar L, Lu B, Geha RS
Mol. Cell. Biol., 2006-07-01;26(14):5214-25.
Species: Mouse
Sample Types: Whole Cells
Applications: Functional Assay
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