Mouse CD45 Alexa Fluor® 405-conjugated Antibody

Catalog #: FAB114V
1 Citation Datasheet / COA / SDS

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FAB114V-025

Detection of CD45 in Mouse Bone Marrow Cells by Flow Cytometry.

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All CD45 Antibodies

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Mouse CD45 Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CD45. Recognizes all CD45 isoforms as well as the known mouse CD45 alloantigens (CD45-1/Ly 5a and CD45-2/Ly 5b) (15).

Source

Monoclonal Rat IgG_2B Clone # 30-F11

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse thymus and spleen cells

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Alexa Fluor 405 (Excitation= 405 nm, Emission= 421 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

5 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CD45 in Mouse Bone Marrow Cells by Flow Cytometry. Mouse bone marrow cells were stained with Rat Anti-Mouse TER-119 APC-conjugated Monoclonal Antibody (Catalog # FAB1125A) and either (A) Rat Anti-Mouse CD45 Alexa Fluor® 405-conjugated Monoclonal Antibody (Catalog # FAB114V) or (B) Rat IgG_2BAlexa Fluor 405 Isotype Control (Catalog # IC013V). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD45

CD45, previously called LCA (Leukocyte Common Antigen), T200, or Ly5 in mouse, is member C of the class 1 (receptor-like) Protein Tyrosine Phosphatase family (PTPRC) (1, 2). It is a variably glycosylated 180-220 kDa transmembrane protein that is abundantly expressed on all nucleated cells of hematopoietic origin (1-3). CD45 has several isoforms, expressed according to cell type, developmental stage and antigenic exposure (1-5). The longest form, CD45RABC (called B220 in mouse), is expressed on B lymphocytes (5). The mouse CD45RABC cDNA encodes 1291 amino acids (aa), including a 23 aa signal sequence, a 541 aa extracellular domain containing the splicing region, a cysteine-rich region and two fibronectin type III domains, a 22 aa transmembrane sequence, and a 705 aa cytoplasmic domain that contains two phosphatase domains, D1 and D2. Only D1 has phosphatase activity. CD45R0 is the shortest form, lacking exons 4, 5 and 6 which encode aa 30-169. It is expressed on memory cells, while intermediate sizes are expressed on other T cells (3, 4, 6). CD45 has been best studied in T cells, where it determines T cell receptor signaling thresholds (3, 6-8). CD45 is moved into or out of the immunological synapse (IS) membrane microdomain depending on the relative influence of interaction with the extracellular galectin lattice or the intracellular actin cytoskeleton (9, 10). Galectin interaction can be fine-tuned by varying usage of the heavily O-glycosylated spliced regions and sialylation of N-linked carbohydrates (4, 9). Within the IS, CD45 dephosphorylates and negatively regulates the src family kinase, LCK (8-10). In other leukocytes, CD45 influences differentiation and links immunoreceptor signaling with cytokine secretion and cell survival, partially overlapping in function with DEP-1/CD148 (11-14). CD45 deletion causes severe immunodeficiency, while point mutations may be associated with autoimmune disorders (6, 7).

References

Anderson, J.N. et al. (2004) FASEB J. 18:8.
Streuli, M. et al. (1987) J. Exp. Med. 166:1548.
Hermiston, M.L. et al. (2003) Annu. Rev. Immunol. 21:107.
Earl, L.A. and L.G. Baum (2008) Immunol. Cell Biol. 86:608.
Ralph, S.J. et al. (1987) EMBO J. 6:1251.
Falahti, R. and D. Leitenberg (2008) J. Immunol. 181:6082.
Tchilian, E.Z. and P.C.L. Beverley (2006) Trends Immunol. 27:146.
McNiell, L. et al. (2007) Immunity 27:425.
Chen, I-J. et al. (2007) J. Biol. Chem. 282:35361.
Freiberg, B.A. et al. (2002) Nat. Immunol. 3:911.
Zhu, J.W. et al. (2008) Immunity 28:183.
Huntington, N.D. et al. (2006) Nat. Immunol. 7:190.
Hesslein, D.G. et al. (2006) Proc. Natl. Acad. Sci. USA 103:7012.
Cross, J.L. et al. (2008) J. Immunol. 180:8020.
Ledbetter, J.A. and L.A. Herzenberg (1979) Immunol. Rev. 47:63.

Long Name

Cluster of Differentiation 45

Entrez Gene IDs

5788 (Human); 19264 (Mouse); 490255 (Canine); 100061950 (Equine)

Alternate Names

B220; CD_antigen: CD45; CD45 antigen; CD45; CD45R; EC 3.1.3.48; EC:3.1.3.48; GP180; LCA; L-CA; Leukocyte common antigen; LY5; protein tyrosine phosphatase, receptor type, c polypeptide; PTPRC; receptor-type tyrosine-protein phosphatase C; T200 Glycoprotein; T200 leukocyte common antigen; T200

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Citation for Mouse CD45 Alexa Fluor® 405-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Clathrin mediates both internalization and vesicular release of triggered T cell receptor at the immunological synapse
Authors: A Kvalvaag, S Valvo, PF Céspedes, DG Saliba, E Kurz, K Korobchevs, ML Dustin
Proceedings of the National Academy of Sciences of the United States of America, 2023-02-02;120(6):e2211368120.
Species: Human
Sample Types: Whole Cells
Applications: ICC