Mouse CD55/DAF PE-conjugated Antibody

Name: Mouse CD55/DAF PE-conjugated Antibody
Brand: R&D Systems
SKU: FAB5376P

Catalog #: FAB5376P Datasheet / COA / SDS

Discontinued Product

FAB5376P has been discontinued.

View all CD55/DAF products.

Detection of CD55/DAF in Mouse Splenocytes by Flow Cytometry.

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All CD55/DAF Antibodies

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Mouse CD55/DAF PE-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CD55/DAF in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human CD55 or recombinant mouse CD97 is observed.

Source

Monoclonal Rat IgG_2A Clone # 583905

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CD55/DAF
Met1-Pro359
Accession # Q61475

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CD55/DAF in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD55/DAF PE-conjugated Monoclonal Antibody (Catalog # FAB5376P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: CD55/DAF

CD55, also known as Decay-accelerating Factor/DAF, is a glycoprotein member of the RCA family of molecules. It is found on all blood cells, select epithelium and endothelium, and serves both as a receptor for CD97, and a negative regulator of the C3 convertases, C4b2a and C3bBb. Mature mouse CD55 is the product of two genes that arose by duplication. There is a 55-70 kDa, 328 amino acid (aa), GPI-linked form (aa 35-362) that is ubiquitously expressed and a 52-56 kDa type I transmembrane (TM) form that shoiws limited expression. The TM form shows the same domain architecture and is 93% aa identical to the GPI-form. At least three GPI gene isoforms exist. One contains a 21 aa 390 substitution for aa 286, a second shows a six aa substitution for aa 339-350, and a third contains a Pro substitution for aa 333-361. Over aa 35-359, mouse CD55 is 66% and 50% aa identical to rat and human CD55, respectively.

Entrez Gene IDs

1604 (Human); 13136 (Mouse)

Alternate Names

CD55 antigen; CD55 molecule, decay accelerating factor for complement (Cromer blood group); CD55; CR; CRdecay accelerating factor for complement (CD55, Cromer blood group system); CROMDAFcomplement decay-accelerating factor; DAF; decay accelerating factor for complement; TC