Mouse CD68/SR-D1 Antibody

Catalog #: MAB10114 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB10114-100
MAB10114-SP

CD68/SR‑D1 in RAW 264.7 and NIH-3T3 Mouse Cell Lines.

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All CD68/SR-D1 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CD68/SR-D1 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CD68 in direct ELISAs.

Source

Recombinant Monoclonal Rabbit IgG Clone # 2449C

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Chinese Hamster Ovary cell line, CHO-derived mouse CD68
Asp21-Pro282
Accession # P31996

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

3-25 µg/mL

See below

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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CD68/SR‑D1 in RAW 264.7 and NIH-3T3 Mouse Cell Lines. CD68/SR-D1 was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line (positive stain) and NIH-3T3 mouse embryonic fibroblast cell line (negative stain) using Rabbit Anti-Mouse CD68/SR-D1 Monoclonal Antibody (Catalog # MAB10114) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Intracellular Staining by Flow Cytometry

Detection of CD68 antibody in Raw264.7 Mouse Cell line antibody by Flow Cytometry.

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Detection of CD68 in Raw264.7 Mouse Cell line by Flow Cytometry. Raw264.7 mouse cell line was stained with Rabbit Anti-Mouse CD68 Monoclonal Antibody (Catalog # MAB10114, filled histogram) or Normal Rabbit IgG Control (Catalog # MAB1050, open histogram) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (1x) (Catalog # FC004) and permeabilized with ice-cold methanol. View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD68/SR-D1

Macrosialin (CD68) is a macrophage-restricted glycosylated transmembrane protein. It belongs to the lamp family and carries out specialized functions in dedicated phagocytic cells. Macrosialin binds to tissue- and organ-specific lectins to allow homing of macrophages to specific targets. Its presence in macrophages is useful in diagnosing conditions related to proliferation or abnormality of these cells.

Entrez Gene IDs

968 (Human); 12514 (Mouse); 287435 (Rat)

Alternate Names

CD_antigen: CD68; CD68 antigen; CD68 molecule; CD68; DKFZp686M18236; gp110; LAMP4; macrophage antigen CD68; Macrosialin; SCARD1; scavenger receptor class D, member 1; SRD1; SR-D1