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Mouse CD81 PE-conjugated Antibody

Catalog #: FAB4865P
1 Citation Datasheet / COA / SDS

Discontinued Product

FAB4865P has been discontinued.
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Detection of CD81 in Mouse Splenocytes by Flow Cytometry.
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Citations (1)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CD81 PE-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CD81 in flow cytometry. Stains mouse CD81 transfectants but not irrelevant transfectants.
Source
Monoclonal Rat IgG2A Clone # 431301
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with mouse CD81
Met1-Tyr236
Accession # NP_598416
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CD81 antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of CD81 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD81 PE-conjugated Monoclonal Antibody (Catalog # FAB4865P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: CD81

CD81, also known as TAPA-1 and Tspan28, is a widely expressed protein in the Tetraspanin family. CD81 is a multifunctional protein that interacts with a variety of other molecules, including Tetraspanins, and is important for organization of the plasma membrane into microdomains. CD81 facilitates B cell and T cell activation and is an integrin-binding adhesion molecule. CD81 expression on lymphocytes is altered during infection by hepatitis C virus or HIV-1 and contributes to the pathogenicity of those viruses. Mouse CD81 shares 92%‑94% aa sequence identity with human and rat CD81.

Entrez Gene IDs
975 (Human); 12520 (Mouse); 25621 (Rat)
Alternate Names
CD81 antigen (target of antiproliferative 1); CD81 antigen; CD81 molecule; CD81; CVID6; TAPA1; TAPA-1; Target of the antiproliferative 1; Tetraspanin-28; TSPAN28; Tspan-28; tspan-28,26 kDa cell surface protein TAPA-1; TSPAN28S5.7

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Citation for Mouse CD81 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Circulating miRNAs in Small Extracellular Vesicles Secreted by a Human Melanoma Xenograft in Mouse Brains
    Authors: L Guglielmi, M Nardella, C Musa, I Cifola, M Porru, B Cardinali, I Iannetti, C Di Pietro, G Bolasco, V Palmieri, L Vilardo, N Panini, F Bonaventur, M Papi, F Scavizzi, M Raspa, C Leonetti, G Falcone, A Felsani, I D'Agnano
    Cancers (Basel), 2020-06-19;12(6):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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