Mouse CD9 Antibody

Catalog #: MAB5218
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5218
MAB5218-SP

Detection of CD9 in D3 Mouse Cell Line by Flow Cytometry.

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All CD9 Antibodies

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Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CD9 Antibody Summary

Species Reactivity

Mouse

Specificity

Stains mouse CD9-transfected cells but not irrelevant transfecants.

Source

Monoclonal Rat IgG_2A Clone # 479608

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with mouse CD9
Pro2-Val226
Accession # P40240

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

Immunohistochemistry

8-25 µg/mL

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CD9 in D3 Mouse Cell Line by Flow Cytometry. D3 mouse embryonic stem cell line was stained with Rat Anti-Mouse CD9 Monoclonal Antibody (Catalog # MAB5218, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram) followed by anti-Rat IgG APC-conjugated antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.

Immunohistochemistry

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CD9 in Mouse Kidney. CD9 was detected in perfusion fixed frozen sections of mouse kidney using Rat Anti-Mouse CD9 Monoclonal Antibody (Catalog # MAB5218) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD9

CD9, also known as motility-related protein-1 (MRP-1), is a 24 kDa glycoprotein of the tetraspanin family that is expressed by a variety of hematopoietic and epithelial cells. It forms homotypic and heterotypic associations with other tetraspanin proteins, some integrins and MHC class II proteins. CD9 has been shown to modulate cellular adhesion, migration and proliferation. The extracellular portions of mouse CD9 share 81% and 88% amino acid identity with corresponding regions of human and rat CD9, respectively.

Entrez Gene IDs

928 (Human); 12527 (Mouse)

Alternate Names

BA2; BA-2/p24 antigen; BTCC-1; CD9 antigen; CD9 molecule; CD9; Cell growth-inhibiting gene 2 protein; DRAP-27; Leukocyte antigen MIC3; MIC3; motility related protein-1,5H9 antigen; Motility-related protein; MRP-1FLJ99568; p24; tetraspanin-29; TSPAN29; TSPAN-29; TSPAN29MIC3CD9 antigen (p24)

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citations for Mouse CD9 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Immuno-digital invasive cleavage assay for analyzing Alzheimer’s amyloid beta -bound extracellular vesicles
Authors: Kohei Yuyama, Hui Sun, Yasuyuki Igarashi, Kenji Monde, Takumi Hirase, Masato Nakayama et al.
Alzheimer's Research & Therapy
Evaluation of Plant Ceramide Species-Induced Exosome Release from Neuronal Cells and Exosome Loading Using Deuterium Chemistry
Authors: Y Murai, T Honda, K Yuyama, D Mikami, K Eguchi, Y Ukawa, S Usuki, Y Igarashi, K Monde
International Journal of Molecular Sciences, 2022-09-15;23(18):.
Species: Human
Sample Types: Whole Cells
Applications: ICC