Mouse CEACAM-2 Antibody

Catalog #: MAB9214 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB9214-100
MAB9214-SP

Detection of CEACAM-2 in HEK293 Human Cell Line Transfected with Mouse CEACAM-2 and eGFP by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CEACAM-2 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CEACAM-2 in direct ELISAs.

Source

Monoclonal Rat IgG₁ Clone # 935610

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CEACAM-2
Gln35-Asp422
Accession # Q925P2

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CEACAM-2 in HEK293 Human Cell Line Transfected with Mouse CEACAM-2 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) mouse CEACAM-2 or (B) irrelevant transfectants and eGFP was stained with Rat Anti-Mouse CEACAM-2 Monoclonal Antibody (Catalog # MAB9214) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). Quadrant markers were set based on control antibody staining (Catalog # MAB005). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CEACAM-2

CEACAM2 is a member of the family of carcinoembryonic antigen-related cell adhesion molecules. CEACAM2 controls energy balance and peripheral insulin action. It is involved in the regulation of feeding behavior particularly in the ventromedial nucleus of hypothalamus (VMH) regulation of food intake. Alternatively spliced transcripts encoding different proteins have been described.

Long Name

Carcinoembryonic Antigen-related Cell Adhesion Molecule 2

Entrez Gene IDs

26367 (Mouse)

Alternate Names

AI267129; Bgp;Bgp2;Carcinoembryonic antigen-related cell adhesion molecule 2;Ceacam2-L; Bgp2; CEACAM2; CEACAM-2; Ceacam2-L