Mouse Chemerin Antibody

Catalog #: MAB23251
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB23251
MAB23251-SP

All Chemerin Antibodies

Product Details

Citations (2)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Chemerin Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Chemerin in ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant human Chemerin, recombinant mouse (rm) Cystatin C, or rmFetuin A is observed.

Source

Monoclonal Rat IgG_2B Clone # 372409

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant mouse Chemerin
Thr17-Ser156
Accession # Q9DD06

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Mouse Chemerin Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Mouse Chemerin Biotinylated Antibody (Catalog # BAM2325)

Standard: Recombinant Mouse Chemerin (aa 17-156) Protein (Catalog # 2325-CM)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Chemerin

Mouse Chemerin, also known as Tazarotene-induced Gene-2 (TIG2), is a new, but distant member of the cystatin superfamily (1‑3). Members of this superfamily contain at least two intrachain disulfide bonds and an alpha -helical structure over a distance of about 100 amino acids (aa) (2, 3). Chemerin is synthesized as a 162 aa precursor that contains a hydrophobic N-terminal sequence, an intervening 140 aa cystatin-fold containing domain, and a six aa C-terminal prosegment (4‑6). Within the cystatin-fold domain there are three intrachain disulfide bonds that contribute to the characteristic fold (4, 7). The precursor molecule is described as undergoing proteolytic processing at both termini by unknown proteases. The N-terminal 16 residue hydrophobic segment is described as being either a signal sequence or a transmembrane (TM) segment for a type II TM protein (5, 8). In either case it gives rise to a soluble proform that undergoes further processing at the C-terminus (5). In mouse, the C-terminal six residues are cleaved, giving rise to a monomeric, 16 kDa heparin-binding bioactive molecule (aa 17‑156) (5‑7). A shorter form has been described in human (7). The activity seems to be concentrated in the nine aa’s preceding the prosegment (aa 148‑156). Retention of the prosegment blocks activity (4). The 140 aa mature segment is known to bind to the G-protein coupled receptor termed ChemR23 (5, 7). Binding results in macrophage and immature dendritic cell chemotaxis (5). The distribution of this receptor is limited to immune APCs, and it is assumed that Chemerin is an inflammatory molecule. It is unclear which cells are actually producing Chemerin, but keratinocytes, endothelial cells and osteoclasts are potential candidates (1, 7). Mature mouse Chemerin shares 67%, 84% and 82% aa sequence identity with human, rat and hamster Chemerin, respectively (6). There is apparently cross-species activity for the protein (6).

References

Nagpal, S. et al. (1997) J. Invest. Dermatol. 109:91.
Storici, P. et al. (1996) Eur. J. Biochem. 238:769.
Zanetti, M. (2004) J. Leukoc. Biol. 75:39.
Wittamer, V. et al. (2004) J. Biol. Chem. 279:9956.
Wittamer, V. et al. (2003) J. Exp. Med. 198:977.
Busmann, A. et al. (2004) J. Chromatog. B 811:217.
Meder, W. et al. (2003) FEBS Lett. 555:495.
Yokoyama-Kobayashi, M. et al. (1999) Gene 228:161.

Long Name

Retinoic Acid Receptor Responder Protein 2

Entrez Gene IDs

5919 (Human); 71660 (Mouse)

Alternate Names

Chemerin; RARRES2; RAR-responsive protein TIG2; retinoic acid receptor responder (tazarotene induced) 2; retinoic acid receptor responder protein 2; Tazarotene-induced gene 2 protein; TIG-2; TIG2HP10433

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Related Research Areas

Citations for Mouse Chemerin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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The methylation status of the chemerin promoter region located from -?252 to +?258ï¿½bp regulates constitutive but not acute-phase cytokine-inducible chemerin expression levels
Authors: K Kwiecien, P Brzoza, M Bak, P Majewski, I Skulimowsk, K Bednarczyk, J Cichy, M Kwitniewsk
Sci Rep, 2020-08-13;10(1):13702.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA Capture
Dynamic and tissue-specific proteolytic processing of chemerin in obese mice
Authors: L Zhao, Y Yamaguchi, WJ Shen, J Morser, LLK Leung
PLoS ONE, 2018-08-30;13(8):e0202780.
Species: Mouse
Sample Types: Recombinant Protein
Applications: ELISA Development (Capture)