Home / CHL-1/L1CAM-2 / Mouse CHL-1/L1CAM-2 Antibody

Mouse CHL-1/L1CAM-2 Antibody

Catalog #: AF2147
25 Citations 3 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2147
AF2147-SP
Detection of Mouse CHL‑1/L1CAM‑2 by Western Blot.
6 Images
Product Details
Citations (25)
FAQs
Supplemental Products
Reviews (3)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CHL-1/L1CAM-2 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CHL‑1/L1CAM‑2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 40% cross-reactivity with recombinant human CHL-1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CHL‑1/L1CAM‑2
Ala25-Gln1043 (Leu227-Gln242 del, Ala243Ser)
Accession # BAC30699
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.25 µg/mL
See below
Simple Western
2.5 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
Perfusion fixed frozen sections of mouse brain (cortex)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse CHL-1/L1CAM-2 antibody by Western Blot. View Larger

Detection of Mouse CHL‑1/L1CAM‑2 by Western Blot. Western blot shows lysates of mouse brain stem tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse CHL-1/L1CAM-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2147) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for CHL-1/L1CAM-2 at approximately 200 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Mouse CHL-1/L1CAM-2 antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of Mouse CHL‑1/L1CAM‑2 by Simple WesternTM. Simple Western lane view shows lysates of mouse brain (cortex) tissue and mouse brain stem tissue, loaded at 0.2 mg/mL. A specific band was detected for CHL-1/L1CAM-2 at approximately 196-201 kDa (as indicated) using 2.5 µg/mL of Goat Anti-Mouse CHL-1/L1CAM-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2147) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Western Blot Detection of Mouse CHL-1/L1CAM-2 by Western Blot View Larger

Detection of Mouse CHL-1/L1CAM-2 by Western Blot BACE1-mediated processing of APP and CHL1 is reduced in cortex of aged BACE1 cKO mice following tamoxifen treatment. Cortex homogenates from TAM- or VEH-treated mice were resolved by SDS-PAGE for Western blot analysis of APP and CHL1 processing. Homogenates from aged-matched BACE+/− and BACE1−/− were also loaded as control samples. APP-FL, pC99 and pC89 were normalized to GAPDH (MAB374) while CHL1-FL and CHL1-NTF were normalized to beta -tubulin (JDR.3B8). Protein amount was normalized to protein levels in control mice injected with vehicle (set at 1). Representative blots of (a) APP-FL (C1/6.1), (b) APP-CTFs (C1/6.1) and (c) CHL1. (d) Densitometry analysis of protein expression. APP processing was reduced in TAM-treated mice as demonstrated by the accumulation of APP-FL (C1/6.1), and reduced levels of the beta CTFs pC99 and pC89. beta CTFs were clearly identified because missing in the BACE1−/− sample. CHL1-FL (AF2147) levels were increased and CHL1-NTF levels were significantly reduced. Furthermore, the CHL1-NTF/CHL1-FL ratio was significantly decreased in TAM-treated mice demonstrating reduced BACE1 processing (VEH n = 7; TAM n = 7). (e) Quantification of A beta x-40 was performed by MSD immunoassay on cortex homogenates and expressed as pMol/g of cortex. The decrease of levels of A beta x-40 in TAM-treated mice was comparable to the one observed in samples collected from young TAM-treated mice (~50% decrease) (VEH n = 7; TAM n = 7). Results were plotted as Mean ± SEM, *p < 0.05; **p < 0.005; ***p < 0.001; ****p < 0.0001; n.s. = not significant, Student’s t test. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31882662), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse CHL-1/L1CAM-2 by Western Blot View Larger

Detection of Mouse CHL-1/L1CAM-2 by Western Blot Axon guidance defects were absent in hippocampus mossy fibers of aged BACE1 cKO mice following partial BACE1 deletion. (a) Coronal sections collected from aged mice were stained with anti-synaptoporin (SPO) antibody (green) and DAPI (blue). Scale bar 50 μm. (b) Quantification of IPB length showed no alteration in TAM-treated mice compared to controls. IPB length was normalized on the length of the CA3 stratum lucidum (VEH n = 8; TAM n = 7, 3 to 4 sections per mouse). (c) Representative microscopy images showing reduced BACE1 (D10E5) expression in the hippocampus of TAM-treated mice. BACE1 signal was totally absent in BACE−/− mice, used as control to evaluate the amount of background in the staining. Scale bar 200 μm. Hippocampus full homogenates from TAM- or VEH-treated mice were resolved by SDS-PAGE for analysis of APP processing and fractionated (soluble and membrane fractions) for the analysis of SEZ6 and CHL1 processing. Homogenates from aged-matched BACE+/− and BACE1−/− were loaded as control samples. Representative blots of (d) APP-FL (C1/6.1) and APP- CTFs (C1/6.1), (e) fractionation blots of sAPP beta (BAWT), SEZ6 (14E5) and CHL1 (AF2147). (f) Densitometry analysis of protein expression. APP processing was reduced in TAM-treated mice as demonstrated by the accumulation of APP-FL (C1/6.1), and reduced levels of the beta CTFs pC99 and pC89, and sAPP beta. beta CTFs and sAPP beta were identified because missing in the BACE1−/− sample. SEZ6 processing was decreased in TAM-treated mice with accumulation of the full length and decreased levels of the ectodomain (SEZ6-NTF) as well as decreased SEZ6-NTF/SEZ6FL ratio. Processing of CHL1 was also impaired as showed by increased of CHL1-FL levels, while CHL1-NTF was not altered. CHL1-NTF/CHL1-FL ratio was significantly decreased. APP-FL, CTFs, SEZ6-NTF and CHL1-NTF were normalized to GAPDH (MAB374), SEZ6-FL and CHL1-FL were normalized to Calnexin (610523) (VEH n = 5; TAM n = 5). (g) A beta x-40 was quantified from hippocampus homogenates by MSD immunoassay. TAM-treated group displayed a significant reduction of A beta x-40 levels (~50% decrease) compared to control (VEH n = 7; TAM n = 7). Results were plotted as Mean ± SEM, **p < 0.005; ***p < 0.001; n.s. = not significant, Student’s t test. DG: dentate gyrus, IPB: infrapyramidal bundle, slu: stratum lucidum, MB: main bundle. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31882662), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse CHL-1/L1CAM-2 by Western Blot View Larger

Detection of Mouse CHL-1/L1CAM-2 by Western Blot BACE1-mediated processing of APP and CHL1 is reduced in cortex of young BACE1 cKO mice following tamoxifen treatment. Cortex homogenates from TAM- or VEH-treated mice were resolved by SDS-PAGE for Western blot analysis of APP and CHL1 processing. Homogenates from aged-matched BACE+/− and BACE1−/− were also loaded as control samples. Representative blots of (a) APP-full length (APP-FL) (C1/6.1), (b) APP-Carboxy Terminal Fragments (CTFs) (C1/6.1) and (c) CHL1. (d) Densitometry analysis of protein expression. Protein amount was normalized to protein levels in control mice (set at 1). APP-FL, pC99 and pC89 were normalized to GAPDH (MAB374) while CHL1-FL and CHL1-NTF were normalized to beta -tubulin (JDR.3B8). APP processing was reduced in TAM-treated mice as demonstrated by the accumulation of APP-FL (C1/6.1), and reduced levels of the beta CTFs pC99 and pC89. beta CTFs were clearly identified because missing in the BACE1−/− sample. CHL1-FL (AF2147) levels were increased while CHL1-N Terminal Fragment (CHL1-NTF) levels were not affected in cortex of TAM-treated mice. However, the CHL1-NTF/CHL1-FL ratio was significantly decreased in TAM-treated mice demonstrating reduced BACE1 processing (VEH n = 8; TAM n = 8). (e) A beta x-40 was quantified from brain homogenates by ELISA (VEH n = 8; TAM n = 8). Levels of A beta x-40 expressed as pMol/g of cortex were significantly reduced in TAM-treated mice (~50% decrease). Results were plotted as Mean ± SEM, ***p < 0.001; ****p < 0.0001; n.s. = not significant, Student’s t test. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31882662), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse Mouse CHL-1/L1CAM-2 Antibody by Western Blot View Larger

Detection of Mouse Mouse CHL-1/L1CAM-2 Antibody by Western Blot Quantitative proteomics in Rab35 cKO P0 hippocampus.a Volcano plot of the TMT-based quantitative proteomes identifying the dysregulated proteins in Rab35 cKO hippocampus in comparison with the control hippocampus (n = 5 mice per genotype). b Number of proteins identified as significantly dysregulated and as either membrane traffic-related or neuronal migration-related. c, d Western blot analysis of control and Rab35 cKO P0 hippocampi using anti-contactin-2, anti-CHL1, and anti-actin antibodies. e, f Quantification of contactin-2 (c) and CHL1 (d) protein levels in control and Rab35 cKO P0 hippocampi. Band intensities of the indicated proteins were normalized to those of actin (n = 9 mice per genotype). Unpaired Student’s t-test; e, p = 0.0153; f, p = 0.0095. g, h Levels of contactin-2 (g) and CHL1 (h) were quantified by targeted MS using the PRM method (n = 5 mice per genotype). Unpaired Student’s t-test; gp = 0.0053; hp = 0.0229. i Western blot analysis of control and Rab35 cKO P0 hippocampus using anti-N-cadherin and anti-actin antibodies. j Quantification of N-cadherin protein levels in the control and Rab35 cKO P0 hippocampus (n = 9 mice per genotypes). Unpaired Student’s t-test, p = 0.9020. k Representative images of DIV 2 hippocampal primary neurons stained for contactin-2 (green), rhodamine-phalloidin (magenta) and DAPI (blue). Scale bar, 20 μm. l Quantification of contactin-2 intensity at the somatic plasma membrane in control (n = 4) and Rab35-deficient (n = 4) cells. Thirty neurons from four different cultures per genotype were analyzed. Mann–Whitney U-test, p = 0.0286. Data represent the mean ± SEM; n.s. not significant (p > 0.05); *p < 0.05; **p < 0.01. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/37085665), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CHL-1/L1CAM-2

Close homolog of L1 (CHL-1), also known as cell adhesion L1-like (CALL) and L1 cell adhesion molecule 2 (L1CAM-2), belongs to the L1 subfamily of immunoglobulin (Ig) superfamily cell adhesion molecules, which also includes L1, neurofascin and NgCAM-related cell adhesion molecule (NrCAM) (1‑3). These molecules are type I transmembrane proteins that have 6 Ig-like domains and 4‑5 fibronectin type III-like (FNIII) domains in their extracellular regions. They also share a highly conserved cytoplasmic region of approximately 110 amino acid (aa) residues containing an ankyrin-binding site. CHL-1 is expressed as a highly glycosylated 185 kDa transmembrane protein by subpopulations of neurons and glia of the central and peripheral nervous system (4, 5). Ectodomain shedding via the metalloprotease-disintegrin ADAM8 releases 165 kDa and 125 kDa soluble CHL-1 fragments, which can diffuse away to function at distant sites (6). CHL-1 is not capable of homotypic interactions, but an extracellular binding partner of CHL-1 has not been identified (4). Human CHL1 has been mapped to chromosome 3p26 and is a candidate gene for 3p- syndrome characterized by mental impairment (7). A missense CHL1 polymorphism associated increased risk of schizophrenia, has also been reported (8). The functional importance of CHL-1 in the nervous system is also evident in CHL-1 deficient mice, which display behavioral abnormalities and show misguided axons within the hippocampus and olfactory tract (9). Enhanced ectodomain-shedding of CHL-1 is also observed in Wobbler mice, the neurodegenerative mutant mice (6). In vitro, soluble or substrate-coated CHL-1 promotes neurite outgrowth and neuronal survival of both cerebellar and hippocampal neurons. Cell surface CHL-1 interacts with integrins in cis to potentiate integrin-dependent cell migration toward extracellular matrix proteins (10). For this enhanced cell motility, CHL-1 linkage to the actin cytoskeleton via interaction between ankyrin and the CHL-1 cytoplasmic region is required.

References
  1. Moos, M. et al. (1988) Nature 334:701.
  2. Holm, J. et al. (1996) Eur. J. Neusci. 8:1613.
  3. Wei, M. et al. (1998) Hum. Genet. 103:355.
  4. Hillenbrand, R. et al. (1999) Eur. J. Neurosci. 11:813.
  5. Liu, Q. et al. (2000) J. Neurosci. 20:7682.
  6. Naus, S. et al. (2004) J. Biol. Chem. 279:16083.
  7. Angeloni, D. et al. (1999) Am. J. Med. Genet. 86:482.
  8. Sakurai, K. et al. (2002) Mol. Psychiatry 7:412.
  9. Montag-Sallaz, M. et al. (2002) Mol. Cell. Biol. 22:7967.
  10. Buhusi, M. et al. (2003) J. Biol. Chem. 278(27):25024.
Long Name
Cell Adhesion Molecule with Homology to L1CAM
Entrez Gene IDs
10752 (Human); 12661 (Mouse)
Alternate Names
CALL; CALLClose homolog of L1; cell adhesion molecule with homology to L1CAM (close homolog of L1); cell adhesion molecule with homology to L1CAM (close homologue of L1); CHL1; CHL-1; FLJ44930; L1CAM-2; L1CAM2L1 cell adhesion molecule 2; MGC132578; neural cell adhesion molecule L1-like protein

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citations for Mouse CHL-1/L1CAM-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

25 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. RAB35 is required for murine hippocampal development and functions by regulating neuronal cell distribution
    Authors: Ikuko Maejima, Taichi Hara, Satoshi Tsukamoto, Hiroyuki Koizumi, Takeshi Kawauchi, Tomoko Akuzawa et al.
    Communications Biology
  2. Reduced secretion of neuronal growth regulator 1 contributes to impaired adipose-neuronal crosstalk in obesity
    Authors: E Duregotti, CM Reumiller, U Mayr, M Hasman, LE Schmidt, SA Burnap, K Theofilato, J Barallobre, A Beran, M Grandoch, A Viviano, M Jahangiri, M Mayr
    Nature Communications, 2022-11-25;13(1):7269.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC, Western Blot
  3. Proteomic and functional analyses of the periodic membrane skeleton in neurons
    Authors: R Zhou, B Han, R Nowak, Y Lu, E Heller, C Xia, AH Chishti, VM Fowler, X Zhuang
    Nature Communications, 2022-06-09;13(1):3196.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  4. Interplay in neural functions of cell adhesion molecule close homolog of L1 (CHL1) and Programmed Cell Death 6 (PDCD6)
    Authors: Gabriele Loers, Thomas Theis, Helen Baixia Hao, Ralf Kleene, Sanjana Arsha, Nina Samuel et al.
    FASEB BioAdvances
  5. Spatiotemporal processing of neural cell adhesion molecules 1 and 2 by BACE1 in vivo
    Authors: W Kim, H Watanabe, S Lomoio, G Tesco
    The Journal of Biological Chemistry, 2021-02-03;0(0):100372.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  6. BACE1 partial deletion induces synaptic plasticity deficit in adult mice
    Authors: S Lombardo, M Chiacchiar, A Tarr, W Kim, T Cao, G Sigal, TW Rosahl, W Xia, PG Haydon, ME Kennedy, G Tesco
    Sci Rep, 2019-12-27;9(1):19877.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  7. Proximity proteomics identifies cancer cell membrane cis ‐molecular complex as a potential cancer target
    Authors: Norihiro Kotani, Arisa Yamaguchi, Tomoko Ohnishi, Ryusuke Kuwahara, Takanari Nakano, Yuka Nakano et al.
    Cancer Science
  8. Axonal growth of midbrain dopamine neurons is modulated by the cell adhesion molecule ALCAM through trans-heterophilic interaction with L1Cam, ChL1 and Semaphorin
    Authors: CR Bye, V Rytova, WF Alsanie, CL Parish, LH Thompson
    J. Neurosci., 2019-07-12;0(0):.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: Cell Culture, IHC
  9. Close Homolog of L1 Regulates Dendritic Spine Density in the Mouse Cerebral Cortex through Semaphorin 3B
    Authors: V Mohan, SD Wade, CS Sullivan, MR Kasten, C Sweetman, R Stewart, Y Truong, M Schachner, PB Manis, PF Maness
    J. Neurosci., 2019-06-10;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Cells, Whole Tissue
    Applications: ICC, IHC, Immunoprecipitation, Western Blot
  10. Axo-axonic Innervation of Neocortical Pyramidal Neurons by GABAergic Chandelier Cells Requires AnkyrinG-Associated L1CAM
    Authors: Yilin Tai, Nicholas B. Gallo, Minghui Wang, Jia-Ray Yu, Linda Van Aelst
    Neuron
  11. BACE1 elevation engendered by GGA3 deletion increases ?-amyloid pathology in association with APP elevation and decreased CHL1 processing in 5XFAD mice
    Authors: W Kim, L Ma, S Lomoio, R Willen, S Lombardo, J Dong, PG Haydon, G Tesco
    Mol Neurodegener, 2018-02-02;13(1):6.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  12. Proteolytic cleavage of transmembrane cell adhesion molecule L1 by extracellular matrix molecule Reelin is important for mouse brain development
    Authors: David Lutz, Ahmed Sharaf, Dagmar Drexler, Hardeep Kataria, Gerrit Wolters-Eisfeld, Bianka Brunne et al.
    Scientific Reports
  13. A beta -dependent reduction of NCAM2-mediated synaptic adhesion contributes to synapse loss in Alzheimer’s disease
    Authors: Iryna Leshchyns’ka, Heng Tai Liew, Claire Shepherd, Glenda M. Halliday, Claire H. Stevens, Yazi D. Ke et al.
    Nature Communications
  14. Antagonistic Effects of BACE1 and APH1B-gamma -Secretase Control Axonal Guidance by Regulating Growth Cone Collapse
    Authors: Soraia Barão, Annette Gärtner, Eduardo Leyva-Díaz, Galina Demyanenko, Sebastian Munck, Tine Vanhoutvin et al.
    Cell Reports
  15. Transcriptome analysis reveals transmembrane targets on transplantable midbrain dopamine progenitors.
    Authors: Bye C, Jonsson M, Bjorklund A, Parish C, Thompson L
    Proc Natl Acad Sci U S A, 2015-03-09;112(15):E1946-55.
    Species: Mouse, Rat
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: Flow Cytometry, IHC
  16. An aberrant sugar modification of BACE1 blocks its lysosomal targeting in Alzheimer's disease.
    Authors: Kizuka Y, Kitazume S, Fujinawa R, Saito T, Iwata N, Saido T, Nakano M, Yamaguchi Y, Hashimoto Y, Staufenbiel M, Hatsuta H, Murayama S, Manya H, Endo T, Taniguchi N
    EMBO Mol Med, 2015-02-01;7(2):175-89.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  17. Endothelial VEGF Sculpts Cortical Cytoarchitecture
    Authors: Suyan Li, Katharina Haigh, Jody J. Haigh, Anju Vasudevan
    The Journal of Neuroscience
  18. beta-Site amyloid precursor protein (APP)-cleaving enzyme 1 (BACE1)-deficient mice exhibit a close homolog of L1 (CHL1) loss-of-function phenotype involving axon guidance defects.
    Authors: Hitt B, Riordan S, Kukreja L, Eimer W, Rajapaksha T, Vassar R
    J Biol Chem, 2012-09-17;287(46):38408-25.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  19. Neuronal cadherin (NCAD) increases sensory neurite formation and outgrowth on astrocytes
    Authors: Toby A. Ferguson, Steven S. Scherer
    Neuroscience Letters
  20. Secretome protein enrichment identifies physiological BACE1 protease substrates in neurons.
    Authors: Kuhn PH, Koroniak K, Hogl S, Colombo A, Zeitschel U, Willem M, Volbracht C, Schepers U, Imhof A, Hoffmeister A, Haass C, Rossner S, Brase S, Lichtenthaler SF
    EMBO J., 2012-06-22;31(14):3157-68.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  21. EphB regulates L1 phosphorylation during retinocollicular mapping.
    Mol. Cell. Neurosci., 2012-05-08;50(2):201-10.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation, Western Blot
  22. CHL1 negatively regulates the proliferation and neuronal differentiation of neural progenitor cells through activation of the ERK1/2 MAPK pathway.
    Authors: Huang X, Zhu LL, Zhao T
    Mol. Cell. Neurosci., 2010-10-08;46(1):296-307.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  23. Phosphatidylinositol 3-kinase/protein kinase Cdelta activation induces close homolog of adhesion molecule L1 (CHL1) expression in cultured astrocytes.
    Authors: Wu J, Wrathall JR, Schachner M
    Glia, 2010-02-01;58(3):315-28.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Neutralization, Western Blot
  24. CHL1 promotes Sema3A-induced growth cone collapse and neurite elaboration through a motif required for recruitment of ERM proteins to the plasma membrane.
    Authors: Schlatter MC, Buhusi M, Wright AG, Maness PF
    J. Neurochem., 2007-11-06;104(3):731-44.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  25. Tau and other proteins found in Alzheimer's disease spinal fluid are linked to retromer-mediated endosomal traffic in mice and humans.
    Authors: Simoes S, Neufeld J. L, et al.
    Sci Transl Med

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Mouse CHL-1/L1CAM-2 Antibody

Average Rating: 5 (Based on 3 Reviews)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Mouse CHL-1/L1CAM-2 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Mouse CHL-1/L1CAM-2 Antibody
By Anonymous on 07/16/2021
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Cortical and thalamic neurons Species: Mouse
Immunocytochemistry/Immunofluorescence Mouse CHL-1/L1CAM-2 Antibody AF2147
Mouse CHL-1/L1CAM-2 Antibody
By Anonymous on 07/09/2021
Application: WB Sample Tested: Brainstem tissue Species: Mouse
Western Blot Mouse CHL-1/L1CAM-2 Antibody AF2147
Mouse CHL‑1/L1CAM‑2 Antibody
By Rob Andrew on 04/26/2016
Application: WB Sample Tested: Brain tissue Species: Mouse

80, 40 and 20ug of total mouse brain homogenate was separated by SDS-PAGE and transfered to PVDF membrane
Block: 1% BSA, 1% FSG PBS-T 1h RT
Primary: 1:1000, 1% BSA, 1% FSG PBS-T O/N 4oC
Secondary: 1:5000,1% BSA, 1% FSG PBS-T 2h RT
(Secondary - DaG 800, Licor)

Western Blot Mouse CHL‑1/L1CAM‑2 Antibody AF2147