Mouse Common gamma Chain/IL-2 R gamma Antibody

Catalog #: MAB7843
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7843
MAB7843-SP

Detection of Mouse Common gamma Chain/IL‑2 R gamma by Western Blot.

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All Common gamma Chain/IL-2 R gamma Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Common gamma Chain/IL-2 R gamma Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Common gamma Chain/ IL-2 R gamma in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Common gamma Chain, recombinant mouse (rm) IL-2 R alpha, or rmIL-2 R beta is observed.

Source

Monoclonal Rat IgG_2A Clone # 644337

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

S. frugiperda insect ovarian cell line Sf21-derived recombinant mouse Common gamma Chain/ IL-2 R gamma
Ser25-Ala263 (predicted)
Accession # P34902

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse Common gamma Chain/IL‑2 R gamma by Western Blot. Western blot shows lysates of mouse lymphocytes. PVDF Membrane was probed with 2 µg/mL of Rat Anti-Mouse Common gamma Chain/IL-2 R gamma Monoclonal Antibody (Catalog # MAB7843) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Common gamma Chain/IL-2 R gamma at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Common gamma Chain/IL-2 R gamma

The Common gamma Chain, also known as CD132, is a transmembrane protein belonging to the hematopoietin receptor family. gamma _c is a signaling component of the functional receptor complexes for IL-2, IL-4, IL-7, IL-9, and IL-15.

Long Name

IL-2 Receptor gamma

Entrez Gene IDs

3561 (Human); 16186 (Mouse)

Alternate Names

CD132 antigen; CD132; CIDX; combined immunodeficiency, X-linked; common cytokine receptor gamma chain; Common gamma Chain; common gamma-chain; cytokine receptor common subunit gamma; gamma(c); IL-2 R gamma; IL-2 receptor subunit gamma; IL2R gamma; IL-2R subunit gamma; IL2RG; IL-2RG; IMD4; interleukin 2 receptor, gamma; Interleukin-2 receptor subunit gamma; P64; SCIDX; SCIDX1; severe combined immunodeficiency

Product Datasheets

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Citation for Mouse Common gamma Chain/IL-2 R gamma Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1