Mouse Complement Factor D/Adipsin Antibody

Catalog #: MAB5430
4 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5430
MAB5430-SP

Detection of Mouse Complement Factor D/Adipsin by Western Blot.

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All Complement Factor D/Adipsin Antibodies

Product Details

Citations (4)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Complement Factor D/Adipsin Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Complement Factor D/Adipsin in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) Complement Factors B, D,H, I or rhGranzyme A is observed.

Source

Monoclonal Rat IgG₁ Clone # 577620

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 cell-derived recombinant mouse Complement Factor D/Adipsin
Ile26-Ser259
Accession # P03953

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human or Recombinant Mouse Complement Factor D, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse Complement Factor D/Adipsin by Western Blot. Western blot shows lysates of mouse adipose tissue. PVDF Membrane was probed with 2 µg/mL of Rat Anti-Mouse Complement Factor D/Adipsin Monoclonal Antibody (Catalog # MAB5430) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Complement Factor D/Adipsin at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Complement Factor D/Adipsin

Complement Factor D (also Adipsin, C3 convertase activator and Properdin factor D) is a 37‑43 kDa member of the S1 peptidase family of enzymes. In mouse, it is secreted by fat cells and helps form C3 convertase, a complex of C3b, Factor B and Factor D. This complex cleaves C3 into C3b and C3a, the latter of which is converted into ASP, a molecule that binds the adipocyte receptor C5L2 and induces triglyceride synthesis. Mature mouse Complement Factor D contains one peptidase S1 domain that cleaves an Arg‑Lys bond. There are at least two potential alternative splice forms. One shows a two aa substitution for aa 1‑71, and a second shows a 15 aa substitution for aa 1‑107. Mature mouse Complement Factor D shares 85% and 68% aa identity with rat and human Complement Factor D, respectively.

Entrez Gene IDs

1675 (Human); 11537 (Mouse); 54249 (Rat)

Alternate Names

Adipsin; ADN; ADNcomplement factor D; AMBP-1; C3 convertase activator; CFD; complement factor D (adipsin); complement factor D preproprotein; Complement Factor D; D component of complement (adipsin); DF; EC 3.4.21; EC 3.4.21.46; PFD; Properdin factor DADIPSIN

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse Complement Factor D/Adipsin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Toll-like Receptor 2 Facilitates Oxidative Damage-Induced Retinal Degeneration
Authors: K Mulfaul, E Ozaki, N Fernando, K Brennan, KR Chirco, E Connolly, C Greene, A Maminishki, RG Salomon, M Linetsky, R Natoli, RF Mullins, M Campbell, SL Doyle
Cell Rep, 2020-02-18;30(7):2209-2224.e5.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization
Extracellular Vesicle-Contained eNAMPT Delays Aging and Extends Lifespan in Mice
Authors: Mitsukuni Yoshida, Akiko Satoh, Jonathan B. Lin, Kathryn F. Mills, Yo Sasaki, Nicholas Rensing et al.
Cell Metabolism
Fat-Produced Adipsin Regulates Inflammatory Arthritis
Authors: Y Li, W Zou, JR Brestoff, N Rohatgi, X Wu, JP Atkinson, CA Harris, SL Teitelbaum
Cell Rep, 2019-06-04;27(10):2809-2816.e3.
Species: Transgenic Mouse
Sample Types: Serum
Applications: Western Blot
Inhibition of the alternative complement pathway preserves photoreceptors after retinal injury
Authors: J. Harry Sweigard, Hidetaka Matsumoto, Kaylee E. Smith, Leo A. Kim, Eleftherios I. Paschalis, Yoko Okonuki et al.
Science Translational Medicine