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Mouse CREG Antibody

Catalog #: AF1697
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1697
AF1697-SP
Product Details
Citations (2)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CREG Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CREG in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross‑reactivity with recombinant human CREG is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse CREG
Arg32-Gln220
Accession # NP_035934
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse CREG

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CREG

CREG is a secreted glycoprotein that belongs to the CREG protein family. It is induced during differentiation of pluripotent cells and is ubiquitously expressed in adult tissues. Mannose-6 phosphate (M6P) modified CREG binds directly to the M6P/IGF2 receptor to inhibit cell growth (1).

References
  1. DiBacco, A. and G. Gill, 2003, Oncogene 22:5436.
Long Name
Cellular Repressor of E1A-stimulated Genes
Entrez Gene IDs
8804 (Human); 433375 (Mouse)
Alternate Names
cellular repressor of E1A-stimulated genes 1CREG; cellular repressor of E1A-stimulated genes; CREG; CREG1; protein CREG1

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Citations for Mouse CREG Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. The secreted inhibitor of invasive cell growth CREG1 is negatively regulated by cathepsin proteases
    Authors: Alejandro Gomez-Auli, Larissa Elisabeth Hillebrand, Daniel Christen, Sira Carolin Günther, Martin Lothar Biniossek, Christoph Peters et al.
    Cellular and Molecular Life Sciences
  2. Overexpressing cellular repressor of E1A-stimulated genes protects mesenchymal stem cells against hypoxia- and serum deprivation-induced apoptosis by activation of PI3K/Akt.
    Authors: Deng J, Han Y, Yan C, Tian X, Tao J, Kang J, Li S
    Apoptosis, 2010-04-01;15(4):463-73.
    Species: Rat
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot

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