Mouse CRELD1 Antibody

Catalog #: AF4116
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4116
AF4116-SP

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Mouse CRELD1 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CRELD1 in direct ELISAs and Western blots. In Western blots, approximately 15% cross-reactivity with recombinant human CRELD1 is observed and less than 5% cross-reactivity with recombinant mouse CRELD2 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CRELD1
Gln30-Glu362
Accession # Q91XD7

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse CRELD1

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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CRELD1 in Mouse Embryo. CRELD1 was detected in immersion fixed frozen sections of mouse embryo (E13.5) using Goat Anti-Mouse CRELD1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4116) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to the developing heart and is shown at both low (lower panel) and high magnification (upper panel). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CRELD1

CRELD1 (cysteine-rich with EGF-like domain protein 1) is an integral membrane protein that functions as a cell adhesion molecule. It is synthesized as a 420 amino acid (aa) precursor with an N-terminal signal peptide, a large extracellular domain (ECD) with two EGF-like repeats that flank two consecutive furin-like repeats, two‑transmembrane domains separated by one cytoplasmic residue, and a short extracellular C-terminal domain. CRELD1 null-mouse fails to develop a normal heart and missense mutations in human CRELD1 have also been associated with atrioventricular septal defects. The N-terminal ECD of mouse CRELD1 shares 97% and 92% aa sequence identity with the ECD of rat and human CRELD1, respectively.

Long Name

Cysteine-rich Protein With EGF-like Domains 1

Entrez Gene IDs

78987 (Human); 171508 (Mouse); 312638 (Rat)

Alternate Names

atrioventricular septal defect 2; AVSD2; CIRRIN; CRELD1; cysteine-rich with EGF-like domain protein 1; cysteine-rich with EGF-like domains 1; DKFZp566D213

Product Datasheets

Product Datasheet

COA

SDS

Citation for Mouse CRELD1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1