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Mouse CRELD2 Antibody

Catalog #: AF3686
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3686
AF3686-SP
Product Details
Citations (2)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CRELD2 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CRELD2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human CRELD2 is observed and less than 1% cross-reactivity with recombinant mouse CRELD1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CRELD2
Ser23-Leu350
Accession # Q9CYA0
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse CRELD2

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CRELD2

CRELD2 is a ubiquitously expressed protein that is the second member of the CRELD family of matricellular proteins. Mouse CRELD2 is synthesized as a 350 amino acid precursor protein with a signal peptide, a highly conserved domain rich in glutamic acid and tryptophan (WE), and EGF-like repeats. Human CRELD2 has been shown to interact with the alpha 4 subunit of the nicotinic acetylcholine receptor and function as a specific regulator of receptor expression. Mouse CRELD2 shares 77% amino acid sequence homology with the human CRELD2 alpha isoform.

Long Name
Cysteine-rich Protein With EGF-like Domains 2
Entrez Gene IDs
79174 (Human); 76737 (Mouse)
Alternate Names
CRELD2; cysteine-rich with EGF-like domain protein 2; cysteine-rich with EGF-like domains 2; DKFZp667O055; MGC11256

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Citations for Mouse CRELD2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. XBP1 signalling is essential for alleviating mutant protein aggregation in ER-stress related skeletal disease
    Authors: KA Piróg, EP Dennis, CL Hartley, RM Jackson, J Soul, JM Schwartz, JF Bateman, RP Boot-Handf, MD Briggs
    PLoS Genet., 2019-07-01;15(7):e1008215.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Elevated urinary CRELD2 is associated with endoplasmic reticulum stress-mediated kidney disease
    Authors: Y Kim, SJ Park, SR Manson, CA Molina, K Kidd, H Thiessen-P, RJ Perry, H Liapis, S Kmoch, CR Parikh, AJ Bleyer, YM Chen
    JCI Insight, 2017-12-07;2(23):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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