Mouse CRHBP Antibody

Catalog #: AF3087
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3087
AF3087-SP

All CRHBP Antibodies

Product Details

Citations (1)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CRHBP Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CRHBP in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 70% cross-reactivity with recombinant human CRHBP is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CRHBP
Tyr25-Leu322
Accession # Q60571

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse CRHBP

Immunohistochemistry

5-15 µg/mL

Perfusion fixed frozen sections of mouse brain (cortex)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CRHBP

Mouse CRHBP is a 37 kDa, monomeric CRH-binding glycoprotein that is secreted by cells of the rodent adrenal, medulla, cortical neurons and astrocytes, and prolactin positive cells of the pituitary. The mature molecule is 298 amino acids (aa) in length and contains six intrachain disulfide bonds. It binds CRH and CRH-like peptides, either serving as an antagonist for CRH binding to CRH receptor 1, or as a coreceptor for CRH binding to CRH receptor 2. Mature mouse CRHBP shares 94%, 87%, 85% and 80% aa sequence identity with rat, human, canine and bovine CRHBP, respectively.

Long Name

Corticotropin Releasing Hormone Binding Protein

Entrez Gene IDs

1393 (Human)

Alternate Names

corticotropin releasing hormone binding protein; corticotropin-releasing factor-binding protein; Corticotropin-releasing hormone-binding protein; CRFBP; CRFBPcorticotropin releasing hormone-binding protein; CRF-BPCRF-binding protein; CRHBP; CRH-BP

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Mouse CRHBP Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1