Mouse CX3CL1/Fractalkine Chemokine Domain Biotinylated Antibody

Catalog #: BAF472
2 Citations Datasheet / COA / SDS

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BAF472

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Citations (2)

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Mouse CX3CL1/Fractalkine Chemokine Domain Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CX3CL1/Fractalkine in ELISAs and Western blots. In sandwich immunoassays, less than 2% cross‑reactivity with recombinant human (rh) Fractalkine (chemokine domain) is observed, less than 0.3% cross‑reactivity with rhFractalkine (full length) is observed and less than 0.1% cross‑reactivity with recombinant rat Fractalkine (full length and chemokine domain) is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse CX3CL1/Fractalkine (R&D Systems, Catalog # 458-MF)
Leu22-Lys105
Accession # O35188

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse CX3CL1/Fractalkine Full Length (Catalog # 458-MF)

Mouse CX3CL1/Fractalkine Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.1-0.4 µg/mL

Use in combination with:

Capture Reagent: Mouse CX3CL1/Fractalkine Chemokine Domain Antibody (Catalog # MAB571)

Standard: Recombinant Mouse CX3CL1/Fractalkine (Full Length) Protein (Catalog # 472-FF)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CX3CL1/Fractalkine

CX3CL1, also named neurotactin, is a member of the delta chemokine subfamily that contains a novel C-X₃-C motif. Unlike other known chemokines, CX3CL1 is a type 1 membrane protein containing a chemokine domain tethered on a long mucin-like stalk. Mouse CX3CL1 cDNA encodes a 395 amino acid (aa) residue precursor protein with a predicted 24 or an alternate 21 aa residue signal peptide, a 76 aa residue globular chemokine domain, a 236 aa residue stalk region rich in Gly, Pro, Ser and Thr and containing degenerate mucin-like repeats, a 21 aa residue transmembrane segment and a 38 aa residue cytoplasmic domain. The extracellular domain of mouse and human CX3CL1 can potentially be released as a soluble protein by proteolysis at the conserved dibasic motif proximal to the transmembrane region. With the exception of the stalk region, human and mouse CX3CL1 share a high degree of amino acid sequence homology (> 75% sequence identity). CX3CL1 is expressed in various tissues including heart, brain, lung, kidney, skeletal muscle, and testis. In rat brain, CX3CL1 expression was found to be localized principally to neurons. The expression of CX3CL1 was also reported to be up-regulated on activated endothelial cells. Membrane-bound CX3CL1 has been shown to promote adhesion of leukocytes. The soluble chemokine domain of human CX3CL1 was reported to be chemotactic for T cells and monocytes while the soluble chemokine domain of mouse CX3CL1 was reported to chemoattract neutrophils and T-lymphocytes but not monocytes. The gene for mouse CX3CL1 has been mapped to chromosome 11. CX3CR1, previously named V28 or chemokine beta receptor-like 1, has been found to be a specific receptor for CX3CL1. In addition, US28, a 7TM receptor encoded by human cytomegalovirus that binds multiple CC chemokines, has also been shown to bind CX3CL1 with high-affinity.

References

Kledal, T.N. et al. (1998) FEBS Lett. 441:209.
Combadiere, C. et al. (1998) J. Biol. Chem. 273:23799.
Harrison, J.L. et al. (1998) Proc. Natl. Acad. Sci. USA 95:10896.
Rossi, D.L. et al. (1998) Genomics 47:163.

Entrez Gene IDs

6376 (Human); 20312 (Mouse); 89808 (Rat); 102117496 (Cynomolgus Monkey)

Alternate Names

ABCD-3; C3Xkine; chemokine (C-X3-C motif) ligand 1; CX3C membrane-anchored chemokine; CX3CL1; CXC3; CXC3C; FKN; Fractalkine; Neurotactin; neurotactin); NTNsmall inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine; NTTSmall-inducible cytokine D1; SCYD1C-X3-C motif chemokine 1; small inducible cytokine subfamily D (Cys-X3-Cys), member-1

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Citations for Mouse CX3CL1/Fractalkine Chemokine Domain Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Acute Ethanol Exposure during Synaptogenesis Rapidly Alters Medium Spiny Neuron Morphology and Synaptic Protein Expression in the Dorsal Striatum
Authors: E Clabough, J Ingersoll, T Reekes, A Gleichsner, A Ryan
International Journal of Molecular Sciences, 2021-12-28;23(1):.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blots
Effect of fractalkine-Fc delivery in experimental lung metastasis using DNA/704 nanospheres.
Authors: Richard-Fiardo P, Cambien B, Pradelli E
Cancer Gene Ther., 2011-08-26;18(11):761-72.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot