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Mouse CXCL10/IP-10/CRG-2 Antibody

Catalog #: AF-466-NA
43 Citations 2 Reviews Datasheet / COA / SDS
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AF-466-NA
AF-466-SP
Detection of Mouse CXCL10/IP‑10/CRG‑2 by Western Blot.
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Product Details
Citations (43)
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Reviews (2)
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Mouse CXCL10/IP-10/CRG-2 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CXCL10/IP-10/CRG-2 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human CXCL10 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse CXCL10/IP-10/CRG-2
Ile22-Pro98
Accession # P17515
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Neutralization
Measured by its ability to neutralize CXCL10/IP‑10/CRG‑2-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR3. The Neutralization Dose (ND50) is typically 5-25 µg/mL in the presence of 0.5 µg/mL Recombinant Mouse CXCL10/IP‑10/CRG‑2.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse CXCL10/IP-10/CRG-2 antibody by Western Blot. View Larger

Detection of Mouse CXCL10/IP‑10/CRG‑2 by Western Blot. Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse CXCL10/IP-10/CRG-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-466-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CXCL10/IP-10/CRG-2 at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Neutralization Chemotaxis Induced by CXCL10/CRG‑2 and Neutral-ization by Mouse CXCL10/ CRG‑2 Antibody. View Larger

Chemotaxis Induced by CXCL10/CRG‑2 and Neutral-ization by Mouse CXCL10/ CRG‑2 Antibody. Recombinant Mouse CXCL10/ CRG-2 (Catalog # 466-CR) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL10/ CRG-2 (0.5 µg/mL) is neutral-ized (green line) by increasing concentrations of Goat Anti-Mouse CXCL10/CRG-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-466-NA). The ND50 is typically 5-25 µg/mL.

Western Blot Detection of Mouse CXCL10/IP-10/CRG-2 by Western Blot View Larger

Detection of Mouse CXCL10/IP-10/CRG-2 by Western Blot Conditional deletion of astroglial CXCL10. (A) Location of loxP sites in the CXCL10 locus in CXCL10fl/fl mice. (B) Day 17 post-MOG peptide injection (dpi) representative images of immunostaining for CXCL10-Red, GFAP-Green, and DAPI-Blue. (C) Quantification of images for CXCL10 integrated density at 17 dpi (n = 4–7 mice/group). (D and E) qRT/PCR for CXCL10 and CXCL9 in spinal cord of astroglial CXCL10 knockout and littermate control mice at 17 dpi (n = 6 mice/group). (F) Quantification of CXCL10 protein levels in the spinal cord of astroglial CXCL10 knockout and control mice at 14 dpi, by western blot analysis (n = 4 mice/group). (G) Data were normalized to GAPDH, P = 0.046. Vertical bars = SEMs. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24924222), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Mouse CXCL10/IP-10/CRG-2 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Mouse CXCL10/IP-10/CRG-2 by Immunocytochemistry/Immunofluorescence Conditional deletion of astroglial CXCL10. (A) Location of loxP sites in the CXCL10 locus in CXCL10fl/fl mice. (B) Day 17 post-MOG peptide injection (dpi) representative images of immunostaining for CXCL10-Red, GFAP-Green, and DAPI-Blue. (C) Quantification of images for CXCL10 integrated density at 17 dpi (n = 4–7 mice/group). (D and E) qRT/PCR for CXCL10 and CXCL9 in spinal cord of astroglial CXCL10 knockout and littermate control mice at 17 dpi (n = 6 mice/group). (F) Quantification of CXCL10 protein levels in the spinal cord of astroglial CXCL10 knockout and control mice at 14 dpi, by western blot analysis (n = 4 mice/group). (G) Data were normalized to GAPDH, P = 0.046. Vertical bars = SEMs. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24924222), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL10/IP-10/CRG-2

The gene for CRG-2, a mouse homolog of human IP-10, was originally identified as an immediate early gene induced in response to macrophage activation. It has since been shown that CRG-2 mRNA is induced by alpha / beta / gamma -interferons and by lipopolysaccharide in macrophages, astrocytes and microglia. Human IP-10 was also shown to be expressed in activated T-lymphocytes, splenocytes, keratinocytes, osteoblasts, astrocytes, and smooth muscle cells. Mouse CRG-2 cDNA encodes a 98 amino acid (aa) residue precursor protein with a 21 aa residue signal peptide that is cleaved to form the 77 aa residue secreted mature protein. Mature CRG-2 shares approximately 67% amino acid sequence identity with human IP-10. The amino acid sequence of CRG-2 identified the protein as a member of the chemokine alpha subfamily that lacks the ELR domain. CRG-2 has been shown to be a chemoattractant for activated T-lymphocytes. Recently, human IP-10 has also been reported to be a potent inhibitor of angiogenesis and to display a potent thymus-dependent anti-tumor effect. A chemokine receptor specific for IP-10 and MIG (CXCR3) has been cloned and shown to be highly expressed in IL-2-activated T-lymphocytes.

References
  1. Loetscher, M. et al. (1996) J. Exp. Med. 184:963.
  2. Vanguri, P. (1996) J. Neuroimmunol. 56:35.
  3. Sgadari, C. et al. (1996) Blood 87:3877.
Entrez Gene IDs
3627 (Human); 15945 (Mouse)
Alternate Names
C7; chemokine (C-X-C motif) ligand 10; CRG2; CRG-2; CXCL10; gIP-10; IFI10; INP10; IP-10; mob-1; SCYB10

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Citations for Mouse CXCL10/IP-10/CRG-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

43 Citations: Showing 1 - 10
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  1. Immune cells and their inflammatory mediators modify beta cells and cause checkpoint inhibitor-induced diabetes
    Authors: Perdigoto AL, Deng S, Du KC et al.
    JCI insight
  2. Mechano-modulatory synthetic niches for liver organoid derivation
    Authors: G Sorrentino, S Rezakhani, E Yildiz, S Nuciforo, MH Heim, MP Lutolf, K Schoonjans
    Nat Commun, 2020-07-10;11(1):3416.
  3. Oncogenic CXCL10 signalling drives metastasis development and poor clinical outcome.
    Authors: Wightman S, Uppal A, Pitroda S, Ganai S, Burnette B, Stack M, Oshima G, Khan S, Huang X, Posner M, Weichselbaum R, Khodarev N
    Br J Cancer, 2015-06-04;113(2):327-35.
  4. A new genetic strategy for targeting microglia in development and disease
    Authors: McKinsey GL, Lizama CO, Keown-Lang AE et al.
    Elife
  5. NF-kappaB Activator 1 downregulation in macrophages activates STAT3 to promote adenoma-adenocarcinoma transition and immunosuppression in colorectal cancer
    Authors: S Wang, Y Kuai, S Lin, L Li, Q Gu, X Zhang, X Li, Y He, S Chen, X Xia, Z Ruan, C Lin, Y Ding, Q Zhang, C Qi, J Li, X He, JL Pathak, W Zhou, S Liu, L Wang, L Zheng
    Bmc Medicine, 2023-03-29;21(1):115.
  6. The dopamine analogue CA140 alleviates AD pathology, neuroinflammation, and rescues synaptic/cognitive functions by modulating DRD1 signaling or directly binding to Abeta
    Authors: Chae, S;Lee, HJ;Lee, HE;Kim, J;Jeong, YJ;Lin, Y;Kim, HY;Leriche, G;Ehrlich, RS;Lingl, SC;Seo, MD;Lee, YH;Yang, J;Kim, JI;Hoe, HS;
    Journal of neuroinflammation
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  7. Astrocytic TDP-43 dysregulation impairs memory by modulating antiviral pathways and interferon-inducible chemokines
    Authors: A Licht-Mura, SM Meadows, F Palaguachi, SC Song, S Jackvony, Y Bram, C Zhou, RE Schwartz, RC Froemke, AL Orr, AG Orr
    Science Advances, 2023-04-19;9(16):eade1282.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. Altered early immune response after fracture and traumatic brain injury
    Authors: Melanie Haffner-Luntzer, Birte Weber, Kazuhito Morioka, Ina Lackner, Verena Fischer, Chelsea Bahney et al.
    Frontiers in Immunology
  9. Cancer cell autophagy, reprogrammed macrophages, and remodeled vasculature in glioblastoma triggers tumor immunity
    Authors: Agnieszka Chryplewicz, Julie Scotton, Mélanie Tichet, Anoek Zomer, Ksenya Shchors, Johanna A. Joyce et al.
    Cancer Cell
  10. Complement receptor C5aR1 on osteoblasts regulates osteoclastogenesis in experimental postmenopausal osteoporosis
    Authors: Jasmin Maria Bülow, Nikolai Renz, Melanie Haffner-Luntzer, Verena Fischer, Astrid Schoppa, Jan Tuckermann et al.
    Front Endocrinol (Lausanne)
  11. Hepatocyte growth factor derived from senescent cells attenuates cell competition-induced apical elimination of oncogenic cells
    Authors: N Igarashi, K Miyata, TM Loo, M Chiba, A Hanyu, M Nishio, H Kawasaki, H Zheng, S Toyokuni, S Kon, K Moriyama, Y Fujita, A Takahashi
    Oncogene, 2022-07-18;13(1):4157.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  12. Survival of stem cells and progenitors in the intestine is regulated by LPA5-dependent signaling
    Authors: Z Liang, P He, Y Han, CC Yun
    Cellular and Molecular Gastroenterology and Hepatology, 2022-04-04;0(0):.
    Species: Mouse
    Sample Types: Organoid
    Applications: Tissue Culture
  13. CCR2 deficiency alters activation of microglia subsets in traumatic brain injury
    Authors: K Somebang, J Rudolph, I Imhof, L Li, EC Niemi, J Shigenaga, H Tran, TM Gill, I Lo, BA Zabel, G Schmajuk, BT Wipke, S Gyoneva, L Jandreski, M Craft, G Benedetto, ED Plowey, I Charo, J Campbell, CJ Ye, SS Panter, MC Nakamura, W Eckalbar, CL Hsieh
    Cell Reports, 2021-09-21;36(12):109727.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  14. Pro-inflammatory beta cell small extracellular vesicles induce beta cell failure through activation of the CXCL10/CXCR3 axis in diabetes
    Authors: N Javeed, TK Her, MR Brown, P Vanderboom, K Rakshit, AM Egan, A Vella, I Lanza, AV Matveyenko
    Cell Reports, 2021-08-24;36(8):109613.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  15. Boosting immune surveillance by low-dose PI3K inhibitor facilitates early intervention of breast cancer
    Authors: J Wang, Y Zhang, Y Xiao, X Yuan, P Li, X Wang, Y Duan, VL Seewaldt, D Yu
    American journal of cancer research, 2021-05-15;11(5):2005-2024.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  16. The lymph node stromal laminin alpha 5 shapes alloimmunity
    Authors: L Li, MW Shirkey, T Zhang, Y Xiong, W Piao, V Saxena, C Paluskievi, YS Lee, N Toney, BM Cerel, Q Li, T Simon, KD Smith, KL Hippen, BR Blazar, R Abdi, JS Bromberg
    J. Clin. Invest., 2020-05-01;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  17. Impacts of Intralipid on Nanodrug Abraxane Therapy and on the Innate Immune System
    Authors: YJ Chen, CY Tsai, YM Cheng, SW Nieh, TK Yeh, CP Chen, MH Wang, LH Chou, TY Chiu, L Liu, C Ho, CT Chen, TW Liu
    Sci Rep, 2020-02-18;10(1):2838.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  18. Key features and homing properties of NK cells in the liver are shaped by activated iNKT cells
    Authors: S Trittel, BJ Chambers, U Heise, CA Guzmán, P Riese
    Sci Rep, 2019-11-08;9(1):16362.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  19. Senescent hepatocytes enhance natural killer cell activity via the CXCL‑10/CXCR3 axis
    Authors: Jinfeng Zang, Jun Ye, Chi Zhang, Min Sha, Junye Gao
    Experimental and Therapeutic Medicine
  20. Chronic Inflammation Directs an Olfactory Stem Cell Functional Switch from Neuroregeneration to Immune Defense
    Authors: M Chen, RR Reed, AP Lane
    Cell Stem Cell, 2019-09-12;25(4):501-513.e5.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  21. Herpes Simplex Virus Type 2 Infection-Induced Expression of CXCR3 Ligands Promotes CD4+ T Cell Migration and Is Regulated by the Viral Immediate-Early Protein ICP4
    Authors: Mudan Zhang, Xu Deng, Xinmeng Guan, Lanlan Geng, Ming Fu, Binman Zhang et al.
    Frontiers in Immunology
  22. Genome-Wide Association Study Identifies Cxcl Family Members as Partial Mediators of LPS-induced Periodontitis
    Authors: S Hiyari, E Green, C Pan, S Lari, M Davar, R Davis, PM Camargo, S Tetradis, AJ Lusis, FQ Pirih
    J. Bone Miner. Res., 2018-05-22;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  23. SSeCKS/AKAP12 scaffolding functions suppress B16F10-induced peritoneal metastasis by attenuating CXCL9/10 secretion by resident fibroblasts
    Authors: M Muramatsu, L Gao, J Peresie, B Balderman, S Akakura, IH Gelman
    Oncotarget, 2017-08-09;8(41):70281-70298.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  24. IFN? Protects Neurons from Damage in a Murine Model of HIV-1 Associated Brain Injury
    Authors: VE Thaney, AM O'Neill, MM Hoefer, R Maung, AB Sanchez, M Kaul
    Sci Rep, 2017-04-20;7(0):46514.
    Species: Mouse, Rat
    Sample Types: Whole Cells
    Applications: Neutralization
  25. Placental growth factor silencing ameliorates liver fibrosis and angiogenesis and inhibits activation of hepatic stellate cells in a murine model of chronic liver disease
    Authors: X Li, QY Yao, HC Liu, QW Jin, BL Xu, SC Zhang, CT Tu
    J. Cell. Mol. Med., 2017-04-05;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  26. Promoted Interaction of C/EBP alpha with Demethylated Cxcr3 Gene Promoter Contributes to Neuropathic Pain in Mice
    Authors: Bao-Chun Jiang, Li-Na He, Xiao-Bo Wu, Hui Shi, Wen-Wen Zhang, Zhi-Jun Zhang et al.
    The Journal of Neuroscience
  27. Brain inflammation is accompanied by peripheral inflammation in Cstb (-/-) mice, a model for progressive myoclonus epilepsy
    J Neuroinflammation, 2016-11-28;13(1):298.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  28. CXCR3 Blockade Inhibits T Cell Migration into the Skin and Prevents Development of Alopecia Areata
    J Immunol, 2016-07-13;0(0):.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: Flow Cytometry, IHC-P
  29. Desiccating Stress–Induced Chemokine Expression in the Epithelium Is Dependent on Upregulation of NKG2D/RAE-1 and Release of IFN-gamma in Experimental Dry Eye
    Authors: Terry G. Coursey, Ritu Bohat, Flavia L. Barbosa, Stephen C. Pflugfelder, Cintia S. de Paiva
    The Journal of Immunology
  30. Deletion of astroglial CXCL10 delays clinical onset but does not affect progressive axon loss in a murine autoimmune multiple sclerosis model.
    Authors: Mills Ko E, Ma J, Guo F, Miers L, Lee E, Bannerman P, Burns T, Ko D, Sohn J, Soulika A, Pleasure D
    J Neuroinflammation, 2014-06-12;11(0):105.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC, Western Blot
  31. Astrocyte-Derived CXCL10 Drives Accumulation of Antibody-Secreting Cells in the Central Nervous System during Viral Encephalomyelitis
    Authors: Timothy W. Phares, Stephen A. Stohlman, David R. Hinton, Cornelia C. Bergmann
    Journal of Virology
  32. Divergent effects of T cell costimulation and inflammatory cytokine production on autoimmune peripheral neuropathy provoked by Aire deficiency.
    Authors: Zeng X, Nagavalli A, Smith C, Howard J, Su M
    J Immunol, 2013-03-13;190(8):3895-904.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  33. Nonviral delivery of small interfering RNA into pancreas-associated immune cells prevents autoimmune diabetes.
    Authors: Leconet W, Petit P, Peraldi-Roux S, Bresson D
    Mol Ther, 2012-09-18;20(12):2315-25.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  34. Identification of Chemoattractive Factors Involved in the Migration of Bone Marrow-Derived Mesenchymal Stem Cells to Brain Lesions Caused by Prions
    Authors: Chang-Hyun Song, Osamu Honmou, Hidefumi Furuoka, Motohiro Horiuchi
    Journal of Virology
  35. Comprehensive assessment of chemokine expression profiles by flow cytometry.
    Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
    J. Clin. Invest., 2010-02-08;120(3):907-23.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  36. Interferon-gamma and the interferon-inducible chemokine CXCL10 protect against aneurysm formation and rupture.
    Authors: King VL, Lin AY, Kristo F, Anderson TJ, Ahluwalia N, Hardy GJ, Owens AP, Howatt DA, Shen D, Tager AM, Luster AD, Daugherty A, Gerszten RE
    Circulation, 2009-01-12;119(3):426-35.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  37. TWEAK/Fn14 interactions are instrumental in the pathogenesis of nephritis in the chronic graft-versus-host model of systemic lupus erythematosus.
    Authors: Zhao Z, Burkly LC, Campbell S, Schwartz N, Molano A, Choudhury A, Eisenberg RA, Michaelson JS, Putterman C
    J. Immunol., 2007-12-01;179(11):7949-58.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  38. CD8+ T Cells are required for inflammation and destruction in cigarette smoke-induced emphysema in mice.
    Authors: Maeno T, Houghton AM, Quintero PA, Grumelli S, Owen CA, Shapiro SD
    J. Immunol., 2007-06-15;178(12):8090-6.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  39. The direct action of 1alpha,25(OH)2-vitamin D3 on purified mouse Langerhans cells.
    Authors: Fujita H, Asahina A, Komine M, Tamaki K
    Cell. Immunol., 2007-05-15;245(2):70-9.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Functional Assay
  40. Viral infection of the lungs through the eye.
    Authors: Bitko V, Musiyenko A, Barik S
    J. Virol., 2006-10-18;81(2):783-90.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  41. CC chemokines mediate leukocyte trafficking into the central nervous system during murine neurocysticercosis: role of gamma delta T cells in amplification of the host immune response.
    Authors: Cardona AE, Gonzalez PA, Teale JM
    Infect. Immun., 2003-05-01;71(5):2634-42.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  42. Targeting KDM4C enhances CD8+ T cell mediated antitumor immunity by activating chemokine CXCL10 transcription in lung cancer
    Authors: Jie X, Chen Y, Zhao Y et al.
    Journal for immunotherapy of cancer
  43. CNS-Native Myeloid Cells Drive Immune Suppression in the Brain Metastatic Niche through Cxcl10
    Authors: Ian H. Guldner, Qingfei Wang, Lin Yang, Samantha M. Golomb, Zhuo Zhao, Jacqueline A. Lopez et al.
    Cell

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Mouse CXCL10/IP-10/CRG-2 Antibody
By Christina Chuong on 12/16/2021
Application: ELISA Sample Tested: Cell Culture Samples Species: Mouse

Antibody initially had high non-specific background (picture) and then antibody function decreased over a course of time when stored at 4C long term.

ELISA Mouse CXCL10/IP-10/CRG-2 Antibody AF-466-NA
Mouse CXCL10/IP-10/CRG-2 Antibody
By Anonymous on 05/09/2019
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Spleen tissue Species: Mouse