Mouse CXCL10/IP‑10/CRG‑2 Biotinylated Antibody

Catalog #: BAF466
11 Citations Datasheet / COA / SDS

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BAF466

Product Details

Citations (11)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CXCL10/IP‑10/CRG‑2 Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CXCL10/IP‑10/CRG‑2 in ELISAs and Western blots. In sandwich immunoassays, less than 0.5% cross-reactivity with recombinant human IP‑10, recombinant mouse (rm) PF4, rmMIG, rmLIX, and rmI-TAC is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse CXCL10/IP‑10/CRG‑2 (R&D Systems, Catalog # 466-CR)
Ile22-Pro98
Accession # Q548V9

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse CXCL10/IP‑10/CRG‑2 (Catalog # 466-CR)

Mouse CXCL10/IP-10/CRG-2 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.1-0.4 µg/mL

Use in combination with:

Capture Reagent: Mouse CXCL10/IP‑10/CRG‑2 Antibody (Catalog # MAB466)

Standard: Recombinant Mouse CXCL10/IP-10/CRG-2 Protein (Catalog # 466-CR)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL10/IP-10/CRG-2

The gene for CRG-2, a mouse homolog of human IP-10, was originally identified as an immediate early gene induced in response to macrophage activation. It has been shown that CRG-2 mRNA is induced by alpha / beta / gamma -interferons and by lipopolysaccharide in macrophages, astrocytes and microglia. Human IP-10 was also shown to be expressed in activated T-lymphocytes, splenocytes, keratinocytes, osteoblasts, astrocytes, and smooth muscle cells. Mouse CRG-2 cDNA encodes a 98 amino acid (aa) residue precursor protein with a 21 aa residue signal peptide that is cleaved to form the 77 aa residue secreted mature protein. Mature CRG-2 shares approximately 67% amino acid sequence identity with human IP-10. The amino acid sequence of CRG-2 identified the protein as a member of the chemokine alpha subfamily that lacks the ELR domain. CRG-2 has been shown to be a chemoattractant for activated T-lymphocytes. Human IP-10 has also been reported to be a potent inhibitor of angiogenesis and to display a potent thymus-dependent anti-tumor effect. A chemokine receptor specific for IP-10 and MIG (CXCR3) has been cloned and shown to be highly expressed in IL-2-activated T-lymphocytes.

References

Loetscher, M. et al. (1996) J. Exp. Med. 184:963.
Vanguri, P. (1996) J. Neuroimmunol. 56:35.
Sgadari, C. et al. (1996) Blood, 87:3877.

Entrez Gene IDs

3627 (Human); 15945 (Mouse)

Alternate Names

C7; chemokine (C-X-C motif) ligand 10; CRG2; CRG-2; CXCL10; gIP-10; IFI10; INP10; IP-10; mob-1; SCYB10

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse CXCL10/IP‑10/CRG‑2 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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Early growth response 1 deficiency protects host against Pseudomonas aeruginosa lung infection
Authors: Z Pang, R Raudonis, C McCormick, Z Cheng
Infect. Immun., 2019-12-17;0(0):.
Species: Mouse
Sample Types: BALF
Applications: ELISA Detection
Nitrosporeusine analogue ameliorates Chandipura virus induced inflammatory response in CNS via NF?b inactivation in microglia
Authors: AK Verma, TS Waghmare, GR Jachak, SC Philkhana, DS Reddy, A Basu
PLoS Negl Trop Dis, 2018-07-12;12(7):e0006648.
Species: Mouse
Sample Types: Protein
Applications: ELISA Development (Detection)
Activation of stimulator of interferon genes (STING) induces ADAM17-mediated shedding of the immune semaphorin SEMA4D
Authors: K Motani, H Kosako
J. Biol. Chem., 2018-04-04;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
IL-33 Attenuates Sepsis by Inhibiting IL-17 Receptor Signaling through Upregulation of SOCS3
Authors: R Lv, J Zhao, M Lei, D Xiao, Y Yu, J Xie
Cell. Physiol. Biochem., 2017-08-09;42(5):1961-1972.
Species: Mouse
Sample Types: Serum
Applications: ELISA Development (Detection)
Monocyte- and Neutrophil-Derived CXCL10 Impairs Efficient Control of Blood-Stage Malaria Infection and Promotes Severe Disease.
Authors: Ioannidis L, Nie C, Ly A, Ryg-Cornejo V, Chiu C, Hansen D
J Immunol, 2015-12-30;196(3):1227-38.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: ELISA Development (Detection)
Dipeptidylpeptidase 4 inhibition enhances lymphocyte trafficking, improving both naturally occurring tumor immunity and immunotherapy.
Authors: Barreira da Silva R, Laird M, Yatim N, Fiette L, Ingersoll M, Albert M
Nat Immunol, 2015-06-15;16(8):850-8.
Species: Mouse
Sample Types: Plasma
Applications: ELISA Development (Capture)
Colonic patch and colonic SILT development are independent and differentially regulated events.
Authors: Baptista A, Olivier B, Goverse G, Greuter M, Knippenberg M, Kusser K, Domingues R, Veiga-Fernandes H, Luster A, Lugering A, Randall T, Cupedo T, Mebius R
Mucosal Immunol, 2012-09-19;6(3):511-21.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Nonviral delivery of small interfering RNA into pancreas-associated immune cells prevents autoimmune diabetes.
Authors: Leconet W, Petit P, Peraldi-Roux S, Bresson D
Mol Ther, 2012-09-18;20(12):2315-25.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
Radiation-inducible immunotherapy for cancer: senescent tumor cells as a cancer vaccine.
Authors: Meng Y, Efimova EV, Hamzeh KW
Mol. Ther., 2012-02-14;20(5):1046-55.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P
Antagonist of interferon-inducible protein 10/CXCL10 ameliorates the progression of autoimmune sialadenitis in MRL/lpr mice.
Authors: Hasegawa H, Inoue A, Kohno M, Muraoka M, Miyazaki T, Terada M, Nakayama T, Yoshie O, Nose M, Yasukawa M
Arthritis Rheum., 2006-04-01;54(4):1174-83.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P

A distinct and unique transcriptional program expressed by tumor-associated macrophages (defective NF-kappaB and enhanced IRF-3/STAT1 activation).
Authors: Biswas SK, Gangi L, Paul S, Schioppa T, Saccani A, Sironi M, Bottazzi B, Doni A, Vincenzo B, Pasqualini F, Vago L, Nebuloni M, Mantovani A, Sica A
Blood, 2005-11-03;107(5):2112-22.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC