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Mouse CXCL12/SDF-1 alpha Quantikine ELISA Kit

Catalog #: MCX120
91 Citations 11 Reviews Datasheet / COA / SDS
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MCX120
Mouse CXCL12/SDF-1 alpha ELISA Standard Curve
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Citations (91)
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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Mouse CXCL12/SDF-1 alpha Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (10 uL), Serum (50 uL)
Sensitivity
0.069 ng/mL
Assay Range
0.2 - 10 ng/mL (Cell Culture Supernates, Serum)
Specificity
Natural and recombinant mouse SDF-1 alpha
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Mouse SDF-1 alpha Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse SDF-1 alpha in cell culture supernates and mouse serum. It contains E. coli-expressed recombinant mouse SDF-1 alpha and antibodies raised against the recombinant factor. It has been shown to accurately quantitate recombinant mouse SDF-1 alpha. Results obtained using natural SDF-1 alpha showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values of natural mouse SDF-1 alpha.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 1.09 2.6 4.1 1.25 3.15 4.91
Standard Deviation 0.04 0.12 0.21 0.09 0.24 0.37
CV% 3.7 4.6 5.1 7.2 7.6 7.5

Recovery

The recovery of mouse SDF-1 alpha spiked to three different levels in samples throughout the range of the assay was assessed.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 101 98-104

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse SDF-1 alpha were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Mouse CXCL12/SDF-1 alpha ELISA Linearity

Scientific Data

N/A Mouse CXCL12/SDF-1 alpha ELISA Standard Curve View Larger

Mouse CXCL12/SDF-1 alpha ELISA Standard Curve

Product Datasheets

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Product Datasheet
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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CXCL12/SDF-1 alpha

CXCL12/Stromal Cell-derived Factor 1 (SDF-1) is a member of the CXC chemokine ligand superfamily. Six alternatively spliced CXCL12/SDF-1 isoforms have been identified including, CXCL12/SDF-1 alpha, CXCL12/SDF-1 beta, and CXCL12/SDF-1 gamma. CXCL12/SDF-1 alpha is 89 amino acids (aa) in length with a predicted molecular weight of 10 kDa, CXCL12/SDF-1 beta is 93 aa in length with a predicted molecular weight of 10.6 kDa, and CXCL12/SDF-1 gamma is 119 aa in length with a predicted molecular weight of 13.7 kDa. The mouse CXCL12/SDF-1 orthologs share 99% aa sequence identity with the human protein. The alpha and beta isoforms are ubiquitously expressed while the gamma isoform is primarily expressed in the heart. CXCL12/SDF-1 isoforms are chemotactic for T lymphocytes and monocytes. It is cardio protective and has also been shown to hinder CXCR4-mediated HIV infection of T cells.

Entrez Gene IDs:
6387 (Human); 20315 (Mouse); 493806 (Feline)
Alternate Names:
CXCL12 chemokine (C-X-C motif) ligand 12; CXCL12/SDF-1 alpha; IRH; PBSF; SCYB12; SDF1 alpha; SDF1; SDF1a; SDF1B; TLSF; TPAR1
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 50 µL Assay Diluent
  3.   Add 50 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 100 µL Conjugate
  6.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  7.   Aspirate and wash 4 times.
    8. 200 µL Substrate Solution
  8.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
    9. 50 µL Stop Solution
  9.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Mouse CXCL12/SDF-1 alpha Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

91 Citations: Showing 1 - 10
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  1. Smooth muscle cell-derived Cxcl12 directs macrophage accrual and sympathetic innervation to control thermogenic adipose tissue
    Authors: Lee, D;Benvie, AM;Steiner, BM;Kolba, NJ;Ford, JG;McCabe, SM;Jiang, Y;Berry, DC;
    Cell reports
    Species: Mouse
    Sample Types:
  2. Upregulation of IL4-induced gene 1 enzyme by B2 cells during melanoma progression impairs their antitumor properties
    Authors: Bekkat, F;Seradj, M;Lengagne, R;Fiore, F;Kato, M;Lucas, B;Castellano, F;Molinier-Frenkel, V;Richard, Y;Prévost-Blondel, A;
    European journal of immunology
    Species: Mouse
    Sample Types: Cell Lysates
  3. Haematopoietic stem cell numbers are not solely determined by niche availability
    Authors: Takeishi, S;Marchand, T;Koba, WR;Borger, DK;Xu, C;Guha, C;Bergman, A;Frenette, PS;Gritsman, K;Steidl, U;
    bioRxiv : the preprint server for biology
    Species: Mouse, Transgenic Mouse
    Sample Types: Bone Marrow Lavage Fluid
  4. Neutrophil metalloproteinase driven spleen damage hampers infection control of trypanosomiasis
    Authors: Pham, HTT;Magez, S;Choi, B;Baatar, B;Jung, J;Radwanska, M;
    Nature communications
    Species: Mouse
    Sample Types: Tissue Homogenates
  5. High ploidy large cytoplasmic megakaryocytes are hematopoietic stem cells regulators and essential for platelet production
    Authors: SY Heazlewood, T Ahmad, B Cao, H Cao, M Domingues, X Sun, CK Heazlewood, S Li, B Williams, M Fulton, JF White, T Nebl, CM Nefzger, JM Polo, BT Kile, F Kraus, MT Ryan, YB Sun, PFM Choong, SL Ellis, ML Anko, SK Nilsson
    Nature Communications, 2023-04-13;14(1):2099.
    Species: Mouse
    Sample Types: Plasma
  6. METTL3-mediated m6A mRNA methylation regulates neutrophil activation through targeting TLR4 signaling
    Authors: S Luo, C Liao, L Zhang, C Ling, X Zhang, P Xie, G Su, Z Chen, L Zhang, T Lai, J Tang
    Cell Reports, 2023-03-14;42(3):112259.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  7. Runx1 and Runx2 inhibit fibrotic conversion of cellular niches for hematopoietic stem cells
    Authors: Y Omatsu, S Aiba, T Maeta, K Higaki, K Aoki, H Watanabe, G Kondoh, R Nishimura, S Takeda, UI Chung, T Nagasawa
    Nature Communications, 2022-05-12;13(1):2654.
    Species: Mouse
    Sample Types: Tissue Homogenates
  8. Cytokines and Neurodegeneration in Epileptogenesis
    Authors: P Wolinski, D Ksiazek-Wi, A Glabinski
    Brain sciences, 2022-03-12;12(3):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  9. CXCR4-dependent macrophage-to-fibroblast signaling contributes to cardiac diastolic dysfunction in heart failure with preserved ejection fraction
    Authors: N Zhang, Q Ma, Y You, X Xia, C Xie, Y Huang, Z Wang, F Ye, Z Yu, X Xie
    International journal of biological sciences, 2022-01-09;18(3):1271-1287.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  10. CXCR7 Antagonism Reduces Acute Lung Injury Pathogenesis
    Authors: L Pouzol, A Sassi, N Baumlin, M Tunis, DS Strasser, F Lehembre, MM Martinic
    Oncogene, 2021-11-05;12(0):748740.
    Species: Mouse
    Sample Types: Plasma
  11. Nanoparticle-containing Electrospun Nanofibrous Scaffolds for Sustained Release of SDF-1alpha
    Authors: R Molina-Peñ, M Haji Manso, M Najberg, JM Thomassin, B Gueza, C Alvarez-Lo, E Garcion, C Jérôme, F Boury
    International journal of pharmaceutics, 2021-10-17;0(0):121205.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  12. Interleukin-17A Triggers the Release of Platelet-Derived Factors Driving Vascular Endothelial Cells toward a Pro-Angiogenic State
    Authors: A Gatsiou, K Sopova, A Tselepis, K Stellos
    Cells, 2021-07-22;10(8):.
    Species: Human
    Sample Types: Cell Culture Supernates
  13. Tumor-Associated Macrophage Promotes the Survival of Cancer Cells upon Docetaxel Chemotherapy via the CSF1/CSF1R-CXCL12/CXCR4 Axis in Castration-Resistant Prostate Cancer
    Authors: W Guan, F Li, Z Zhao, Z Zhang, J Hu, Y Zhang
    Genes, 2021-05-19;12(5):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  14. Adipocyte-Specific ACKR3 Regulates Lipid Levels in Adipose Tissue
    Authors: S Gencer, Y Döring, Y Jansen, S Bayasgalan, O Schengel, M Müller, LJF Peters, C Weber, EPC van der Vo
    Biomedicines, 2021-04-06;9(4):.
    Species: Mouse
    Sample Types: Plasma
  15. Effects of protein malnutrition on haematopoietic regulatory activity of bone marrow mesenchymal stem cells
    Authors: AA Hastreiter, GG Dos Santos, EN Makiyama, EWC Santos, P Borelli, RA Fock
    The Journal of nutritional biochemistry, 2021-03-08;0(0):108626.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  16. FGF21 promotes ischaemic angiogenesis and endothelial progenitor cells function under diabetic conditions in an AMPK/NAD+-dependent manner
    Authors: Q Dai, X Fan, X Meng, S Sun, Y Su, X Ling, X Chen, K Wang, X Dai, C Zhang, S Da, G Zhang, C Gu, H Chen, J He, H Hu, L Yu, X Pan, Y Tan, X Yan
    Journal of Cellular and Molecular Medicine, 2021-02-17;0(0):.
    Species: Mouse
    Sample Types: Plasma
  17. Spinal cord injury causes chronic bone marrow failure
    Authors: RS Carpenter, JM Marbourg, FH Brennan, KA Mifflin, JCE Hall, RR Jiang, XM Mo, M Karunasiri, MH Burke, AM Dorrance, PG Popovich
    Nat Commun, 2020-07-24;11(1):3702.
    Species: Mouse
    Sample Types: Bone Marrow Lysate
  18. Chemokine receptor CXCR7 non-cell-autonomously controls pontine neuronal migration and nucleus formation
    Authors: Y Zhu, T Hirata, F Mackay, F Murakami
    Sci Rep, 2020-07-16;10(1):11830.
    Species: Mouse
    Sample Types: Tissue Lysate
  19. Pbrm1 Steers Mesenchymal Stromal Cell Osteolineage Differentiation by Integrating PBAF-Dependent Chromatin Remodeling and BMP/TGF-&beta Signaling
    Authors: S Sinha, M Biswas, SS Chatterjee, S Kumar, A Sengupta
    Cell Rep, 2020-04-28;31(4):107570.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  20. Myocardium-targeted transplantation of PHD2 shRNA-modified bone mesenchymal stem cells through ultrasound-targeted microbubble destruction protects the heart from acute myocardial infarction
    Authors: Z Sun, Y Xie, RJ Lee, Y Chen, Q Jin, Q Lv, J Wang, Y Yang, Y Li, Y Cai, R Wang, Z Han, L Zhang, M Xie
    Theranostics, 2020-04-06;10(11):4967-4982.
    Species: Rat
    Sample Types: Cell Culture Supernants
  21. Low-Energy Extracorporeal Shock Wave Ameliorates Streptozotocin Induced Diabetes and Promotes Pancreatic Beta Cells Regeneration in a Rat Model
    Authors: CC Hsiao, CC Lin, YS Hou, JY Ko, CJ Wang
    Int J Mol Sci, 2019-10-05;20(19):.
    Species: Rat
    Sample Types: Tissue Lysates
  22. Mesenchymal stem cell-derived conditioned medium attenuate angiotensin II-induced aortic aneurysm growth by modulating macrophage polarization
    Authors: YZ Zhou, Z Cheng, Y Wu, QY Wu, XB Liao, Y Zhao, JM Li, XM Zhou, XM Fu
    J. Cell. Mol. Med., 2019-10-04;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  23. Hematopoietic-Extrinsic Cues Dictate Circadian Redistribution of Mature and Immature Hematopoietic Cells in Blood and Spleen
    Authors: M Stenzinger, D Karpova, C Unterraine, S Harenkamp, E Wiercinska, K Hoerster, M Pfeffer, E Maronde, H Bonig
    Cells, 2019-09-05;8(9):.
    Species: Mouse
    Sample Types: Plasma
  24. Evaluation of Neurosecretome from Mesenchymal Stem Cells Encapsulated in Silk Fibroin Hydrogels
    Authors: Y Martín-Mar, L Fernández-, MH Sanchez-Re, N Marí-Buyé, FJ Rojo, J Pérez-Rigu, M Ramos, GV Guinea, F Panetsos, D González-N
    Sci Rep, 2019-06-19;9(1):8801.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  25. CXCL12-CXCR4 pathway activates brown adipocytes and induces insulin resistance in CXCR4-deficient mice under high-fat diet
    Authors: K Kurita, K Ishikawa, K Takeda, M Fujimoto, H Ono, J Kumagai, H Inoue, H Yokoh, K Yokote
    Sci Rep, 2019-04-16;9(1):6165.
    Species: Mouse
    Sample Types: Serum
  26. The Release of Adipose Stromal Cells from Subcutaneous Adipose Tissue Regulates Ectopic Intramuscular Adipocyte Deposition
    Authors: A Girousse, M Gil-Ortega, V Bourlier, C Bergeaud, Q Sastourné-, C Moro, C Barreau, C Guissard, J Vion, E Arnaud, JP Pradère, N Juin, L Casteilla, C Sengenès
    Cell Rep, 2019-04-09;27(2):323-333.e5.
    Species: Mouse
    Sample Types: Plasma
  27. Optimal design, anti-tumour efficacy and tolerability of anti-CXCR4 antibody drug conjugates
    Authors: MJ Costa, J Kudaravall, JT Ma, WH Ho, K Delaria, C Holz, A Stauffer, AG Chunyk, Q Zong, E Blasi, B Buetow, TT Tran, K Lindquist, M Dorywalska, A Rajpal, DL Shelton, P Strop, SH Liu
    Sci Rep, 2019-02-21;9(1):2443.
    Species: Mouse
    Sample Types: Serum
  28. JNK signalling mediates aspects of maternal immune activation: importance of maternal genotype in relation to schizophrenia risk
    Authors: RL Openshaw, J Kwon, A McColl, JM Penninger, J Cavanagh, JA Pratt, BJ Morris
    J Neuroinflammation, 2019-01-28;16(1):18.
    Species: Mouse
    Sample Types: Plasma
  29. Aging Suppresses Skin-Derived Circulating SDF1 to Promote Full-Thickness Tissue Regeneration
    Authors: MA Nishiguchi, CA Spencer, DH Leung, TH Leung
    Cell Rep, 2018-09-25;24(13):3383-3392.e5.
    Species: Mouse
    Sample Types: Serum
  30. Decellularized Diaphragmatic Muscle Drives a Constructive Angiogenic Response In Vivo
    Authors: ME Alvarèz Fa, M Piccoli, C Franzin, A Sgrò, A Dedja, L Urbani, E Bertin, C Trevisan, P Gamba, AJ Burns, P De Coppi, M Pozzobon
    Int J Mol Sci, 2018-04-28;19(5):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  31. Alcohol exposure decreases osteopontin expression during fracture healing and osteopontin-mediated mesenchymal stem cell migration in vitro
    Authors: RM Natoli, H Yu, MC Meislin, P Abbasnia, P Roper, A Vuchkovska, X Xiao, SR Stock, JJ Callaci
    J Orthop Surg Res, 2018-04-27;13(1):101.
    Species: Mouse
    Sample Types: Tissue Lysates
  32. Tumor-independent host secretomes induced by angiogenesis and immune-checkpoint inhibitors
    Authors: M Mastri, CR Lee, A Tracz, RS Kerbel, M Dolan, Y Shi, JML Ebos
    Mol. Cancer Ther., 2018-04-25;0(0):.
    Species: Mouse
    Sample Types: Plasma
  33. SDF-1 is an Autocrine Insulin-Desensitizing Factor in Adipocytes
    Authors: J Shin, A Fukuhara, T Onodera, S Kita, C Yokoyama, M Otsuki, I Shimomura
    Diabetes, 2018-03-26;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Capture
  34. A mouse model for evaluation of efficacy and concomitant toxicity of anti-human CXCR4 therapeutics
    Authors: MJ Costa, J Kudaravall, WH Liu, J Stock, S Kong, SH Liu
    PLoS ONE, 2018-03-19;13(3):e0194688.
    Species: Mouse
    Sample Types: Serum
  35. Anti-CD69 therapy induces rapid mobilization and high proliferation of HSPCs through S1P and mTOR
    Authors: L Notario, E Alari-Pahi, A Albentosa, M Leiva, G Sabio, P Lauzurica
    Leukemia, 2018-02-27;0(0):.
    Species: Mouse
    Sample Types: Plasma
  36. Mesenchymal stem cells over-expressing cxcl12 enhance the radioresistance of the small intestine
    Authors: P Chang, B Zhang, L Shao, W Song, W Shi, L Wang, T Xu, D Li, X Gao, Y Qu, L Dong, J Wang
    Cell Death Dis, 2018-02-05;9(2):154.
    Species: Mouse
    Sample Types: Serum
  37. Neuroprotective effects of ellagic acid on cuprizone-induced acute demyelination through limitation of microgliosis, adjustment of CXCL12/IL-17/IL-11 axis and restriction of mature oligodendrocytes apoptosis
    Authors: N Sanadgol, F Golab, Z Tashakkor, N Taki, S Moradi Kou, A Mostafaie, M Mehdizadeh, M Abdollahi, G Taghizadeh, M Sharifzade
    Pharm Biol, 2017-12-01;55(1):1679-1687.
    Species: Mouse
    Sample Types: Tissue Homogenates
  38. Prevention of breast cancer skeletal metastases with parathyroid hormone
    Authors: S Swami, J Johnson, LA Bettinson, T Kimura, H Zhu, MA Albertelli, RW Johnson, JY Wu
    JCI Insight, 2017-09-07;2(17):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  39. Comprehensive comparison of three different animal models for systemic inflammation
    Authors: S Seemann, F Zohles, A Lupp
    J. Biomed. Sci., 2017-08-24;24(1):60.
    Species: Mouse
    Sample Types: Serum
  40. Sitagliptin-mediated preservation of endothelial progenitor cell function via augmenting autophagy enhances ischaemic angiogenesis in diabetes
    Authors: X Dai, J Zeng, X Yan, Q Lin, K Wang, J Chen, F Shen, X Gu, Y Wang, J Chen, K Pan, L Cai, KA Wintergers, Y Tan
    J. Cell. Mol. Med., 2017-08-10;0(0):.
    Species: Mouse
    Sample Types: Plasma
  41. Sitagliptin, a dipeptidyl peptidase-4 inhibitor, suppresses CXCL5 and SDF-1 and does not accelerate intestinal neoplasia formation in Apc(Min/+) mice fed a high-fat diet
    Authors: K Fujiwara, T Inoue, Y Henmi, Y Hirata, Y Naka, A Hara, K Kakimoto, S Nouda, T Okada, K Kawakami, T Takeuchi, K Higuchi
    Oncol Lett, 2017-08-01;14(4):4355-4360.
    Species: Mouse
    Sample Types: Plasma
  42. Lymphoid differentiation of hematopoietic stem cells requires efficient Cxcr4 desensitization
    Authors: C Freitas, M Wittner, J Nguyen, V Rondeau, V Biajoux, ML Aknin, F Gaudin, S Beaussant-, Y Bertrand, C Bellanné-C, J Donadieu, F Bachelerie, M Espéli, A Dalloul, F Louache, K Balabanian
    J. Exp. Med., 2017-05-26;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  43. Cannabinoid CB1 receptor overactivity contributes to the pathogenesis of idiopathic pulmonary fibrosis
    Authors: R Cinar, BR Gochuico, MR Iyer, T Jourdan, T Yokoyama, JK Park, NJ Coffey, H Pri-Chen, G Szanda, Z Liu, K Mackie, WA Gahl, G Kunos
    JCI Insight, 2017-04-20;2(8):.
    Species: Mouse
    Sample Types: BALF
  44. Neuronal p38? mediates synaptic and cognitive dysfunction in an Alzheimer's mouse model by controlling ?-amyloid production
    Authors: S Colié, S Sarroca, R Palenzuela, I Garcia, A Matheu, R Corpas, CG Dotti, JA Esteban, C Sanfeliu, AR Nebreda
    Sci Rep, 2017-03-31;7(0):45306.
    Species: Mouse
    Sample Types: Tissue Homogenates
  45. Dual Affinity Heparin-Based Hydrogels Achieve Pro-Regenerative Immunomodulation and Microvascular Remodeling
    Authors: ME Ogle, JR Krieger, LE Tellier, J McFaline-F, JS Temenoff, EA Botchwey
    ACS Biomater Sci Eng, 2017-02-20;4(4):1241-1250.
    Species: Mouse
    Sample Types: Hydrogel Supernates
  46. Immunological effects of vaccines combined with granulocyte colony-stimulating factor on a murine WEHI-3 leukemia model
    Authors: J Chen, M Zhang, F Zhou, J Wang, B Niu, W Zhang
    Oncol Lett, 2017-02-13;13(4):2323-2329.
    Species: Mouse
    Sample Types: Cell Lysates
  47. Sphingosine-1-phosphate Receptor-3 Supports Hematopoietic Stem and Progenitor Cell Residence within the Bone Marrow Niche
    Stem Cells, 2017-01-19;0(0):.
    Species: Mouse
    Sample Types: Plasma
  48. The DPP4 Inhibitor Linagliptin Protects from Experimental Diabetic Retinopathy
    PLoS ONE, 2016-12-12;11(12):e0167853.
    Species: Mouse, Rat
    Sample Types: Tissue Homogenates
  49. Absence of CCR2 results in an inflammaging environment in young mice with age-independent impairments in muscle regeneration
    J Leukoc Biol, 2016-08-16;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  50. Direct Renin Inhibition with Aliskiren Improves Ischemia-Induced Neovasculogenesis in Diabetic Animals via the SDF-1 Related Mechanism.
    Authors: Chang T, Wu T, Huang P, Lin C, Chen J, Lin L, Lin S, Chen J
    PLoS ONE, 2015-08-25;10(8):e0136627.
    Species: Mouse
    Sample Types: Plasma
  51. Intra-Arterial Transplantation of Allogeneic Mesenchymal Stem Cells Mounts Neuroprotective Effects in a Transient Ischemic Stroke Model in Rats: Analyses of Therapeutic Time Window and Its Mechanisms.
    Authors: Toyoshima A, Yasuhara T, Kameda M, Morimoto J, Takeuchi H, Wang F, Sasaki T, Sasada S, Shinko A, Wakamori T, Okazaki M, Kondo A, Agari T, Borlongan C, Date I
    PLoS ONE, 2015-06-15;10(6):e0127302.
    Species: Rat
    Sample Types: Tissue Homogenates
  52. Notch Receptor-Ligand Engagement Maintains Hematopoietic Stem Cell Quiescence and Niche Retention.
    Authors: Wang W, Yu S, Zimmerman G, Wang Y, Myers J, Yu V, Huang D, Huang X, Shim J, Huang Y, Xin W, Qiao P, Yan M, Xin W, Scadden D, Stanley P, Lowe J, Huang A, Siebel C, Zhou L
    Stem Cells, 2015-05-13;33(7):2280-93.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  53. SHIP1-expressing mesenchymal stem cells regulate hematopoietic stem cell homeostasis and lineage commitment during aging.
    Authors: Iyer S, Brooks R, Gumbleton M, Kerr W
    Stem Cells Dev, 2015-02-05;24(9):1073-81.
    Species: Mouse
    Sample Types: Serum
  54. Transplanted bone marrow-derived circulating PDGFRalpha+ cells restore type VII collagen in recessive dystrophic epidermolysis bullosa mouse skin graft.
    Authors: Iinuma S, Aikawa E, Tamai K, Fujita R, Kikuchi Y, Chino T, Kikuta J, McGrath J, Uitto J, Ishii M, Iizuka H, Kaneda Y
    J Immunol, 2015-01-19;194(4):1996-2003.
    Species: Mouse
    Sample Types: Serum
  55. Resolving cancer-stroma interfacial signalling and interventions with micropatterned tumour-stromal assays.
    Authors: Shen K, Luk S, Hicks D, Elman J, Bohr S, Iwamoto Y, Murray R, Pena K, Wang F, Seker E, Weissleder R, Yarmush M, Toner M, Sgroi D, Parekkadan B
    Nat Commun, 2014-12-09;5(0):5662.
    Species: Mouse
    Sample Types: Tissue Homogenates
  56. Heparan sulfate inhibits hematopoietic stem and progenitor cell migration and engraftment in mucopolysaccharidosis I.
    Authors: Watson H, Holley R, Langford-Smith K, Wilkinson F, van Kuppevelt T, Wynn R, Wraith J, Merry C, Bigger B
    J Biol Chem, 2014-10-30;289(52):36194-203.
    Species: Mouse
    Sample Types: Complex Sample Type
  57. Low intensity pulsed ultrasound enhanced mesenchymal stem cell recruitment through stromal derived factor-1 signaling in fracture healing.
    Authors: Wei F, Leung K, Li G, Qin J, Chow S, Huang S, Sun M, Qin L, Cheung W
    PLoS ONE, 2014-09-02;9(9):e106722.
    Species: Rat
    Sample Types: Cell Culture Supernates
  58. A novel molecule Me6TREN promotes angiogenesis via enhancing endothelial progenitor cell mobilization and recruitment.
    Authors: Chen H, Wang S, Zhang J, Ren X, Zhang R, Shi W, Lv Y, Zhou Y, Yan X, Chen L, He L, Zhang B, Nan X, Yue W, Li Y, Pei X
    Sci Rep, 2014-08-28;4(0):6222.
    Species: Mouse
    Sample Types: Plasma
  59. Stimulation of endogenous cardioblasts by exogenous cell therapy after myocardial infarction.
    Authors: Malliaras K, Ibrahim A, Tseliou E, Liu W, Sun B, Middleton R, Seinfeld J, Wang L, Sharifi B, Marban E
    EMBO Mol Med, 2014-04-06;6(6):760-77.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  60. IL-17-induced CXCL12 recruits B cells and induces follicle formation in BALT in the absence of differentiated FDCs.
    Authors: Fleige H, Ravens S, Moschovakis G, Bolter J, Willenzon S, Sutter G, Haussler S, Kalinke U, Prinz I, Forster R
    J Exp Med, 2014-03-24;211(4):643-51.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  61. C/EBPbeta mediates osteoclast recruitment by regulating endothelial progenitor cell expression of SDF-1alpha.
    Authors: Fu S, Pang H, Xu J, Wu X
    PLoS ONE, 2014-03-11;9(3):e91217.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  62. CCAAT/enhancer-binding protein beta expressed by bone marrow mesenchymal stromal cells regulates early B-cell lymphopoiesis.
    Authors: Yoshioka S, Miura Y, Yao H, Satake S, Hayashi Y, Tamura A, Hishita T, Ichinohe T, Hirai H, Takaor-Kondo A, Maekawa T
    Stem Cells, 2014-03-01;32(3):730-40.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  63. SDF1-a facilitates Lin-/Sca1+ cell homing following murine experimental cerebral ischemia.
    Authors: Mocco J, Afzal A, Ansari S, Wolfe A, Caldwell K, Connolly E, Scott E
    PLoS ONE, 2014-01-20;9(1):e85615.
    Species: Mouse
    Sample Types: Tissue Homogenates
  64. Klotho deficiency disrupts hematopoietic stem cell development and erythropoiesis.
    Authors: Vadakke Madathil S, Coe L, Casu C, Sitara D
    Am J Pathol, 2014-01-08;184(3):827-41.
    Species: Mouse
    Sample Types: Serum
  65. Protective effects of mesenchymal stem cells with CXCR4 up-regulation in a rat renal transplantation model.
    Authors: Cao, Zhiqiang, Zhang, Geng, Wang, Fuli, Liu, Hongbao, Liu, Long, Han, Yaling, Zhang, Jian, Yuan, Jianlin
    PLoS ONE, 2013-12-30;8(12):e82949.
    Species: Mouse
    Sample Types: Tissue Homogenates
  66. The Spiegelmer NOX-A12, a novel CXCL12 inhibitor, interferes with chronic lymphocytic leukemia cell motility and causes chemosensitization.
    Authors: Hoellenriegel J, Zboralski D, Maasch C, Rosin N, Wierda W, Keating M, Kruschinski A, Burger J
    Blood, 2013-11-25;123(7):1032-9.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  67. Inhibition of the CXCL12/CXCR4-axis as preventive therapy for radiation-induced pulmonary fibrosis.
    Authors: Shu, Hui-Kuo, Yoon, Younghyo, Hong, Samuel, Xu, Kaiming, Gao, Huiying, Hao, Chunhai, Torres-Gonzalez, Edilson, Nayra, Cardenes, Rojas, Mauricio, Shim, Hyunsuk
    PLoS ONE, 2013-11-07;8(11):e79768.
    Species: Mouse
    Sample Types: BALF
  68. SDF-1alpha mediates wound-promoted tumor growth in a syngeneic orthotopic mouse model of breast cancer.
    Authors: Stuelten C, Cervoni-Curet F, Busch J, Sutton E, Webster J, Kavalukas S, Wakefield L, Barbul A, Niederhuber J
    PLoS ONE, 2013-04-11;8(4):e60919.
    Species: Mouse
    Sample Types: Tissue Secretion
  69. Activator protein-2alpha mediates carbon monoxide-induced stromal cell-derived factor-1alpha expression and vascularization in ischemic heart.
    Authors: Lin H, Chen Y, Chiang M, Huang P, Chau L
    Arterioscler Thromb Vasc Biol, 2013-02-07;33(4):785-94.
    Species: Mouse
    Sample Types: Tissue Homogenates
  70. Host-derived MMP-13 exhibits a protective role in lung metastasis of melanoma cells by local endostatin production.
    Authors: Fukuda H, Mochizuki S, Abe H, Okano HJ, Hara-Miyauchi C, Okano H, Yamaguchi N, Nakayama M, D'Armiento J, Okada Y
    Br. J. Cancer, 2011-10-20;105(10):1615-24.
    Species: Mouse
    Sample Types: Serum
  71. JAM-B regulates maintenance of hematopoietic stem cells in the bone marrow.
    Authors: Arcangeli ML, Frontera V, Bardin F, Obrados E, Adams S, Chabannon C, Schiff C, Mancini SJ, Adams RH, Aurrand-Lions M
    Blood, 2011-08-25;118(17):4609-19.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  72. Conditional HIF-1 induction produces multistage neovascularization with stage-specific sensitivity to VEGFR inhibitors and myeloid cell independence.
    Authors: Oladipupo SS, Hu S, Santeford AC, Yao J, Kovalski JR, Shohet RV, Maslov K, Wang LV, Arbeit JM
    Blood, 2011-02-09;117(15):4142-53.
    Species: Human, Mouse
    Sample Types: Plasma, Tissue Homogenates
  73. Application of the chemokine CXCL12 expression plasmid restores wound healing to near normal in a diabetic mouse model.
    Authors: Restivo TE, Mace KA, Harken AH, Young DM
    J Trauma, 2010-08-01;69(2):392-8.
    Species: Mouse
    Sample Types: Serum
  74. Novel insight into stem cell mobilization-plasma sphingosine-1-phosphate is a major chemoattractant that directs the egress of hematopoietic stem progenitor cells from the bone marrow and its level in peripheral blood increases during mobilization due to activation of complement cascade/membrane attack complex.
    Authors: Ratajczak MZ, Lee H, Wysoczynski M, Wan W, Marlicz W, Laughlin MJ, Kucia M, Janowska-Wieczorek A, Ratajczak J
    Leukemia, 2010-04-01;24(5):976-85.
    Species: Mouse
    Sample Types: Plasma
  75. CXCR4 and CXCR7 regulate angiogenesis and CT26.WT tumor growth independent from SDF-1.
    Authors: Kollmar O, Rupertus K, Scheuer C, Nickels RM, Haberl GC, Tilton B, Menger MD, Schilling MK
    Int. J. Cancer, 2010-03-15;126(6):1302-15.
    Species: Mouse
    Sample Types: Serum
  76. Podocytes produce homeostatic chemokine stromal cell-derived factor-1/CXCL12, which contributes to glomerulosclerosis, podocyte loss and albuminuria in a mouse model of type 2 diabetes.
    Authors: Sayyed SG, Hagele H, Kulkarni OP, Endlich K, Segerer S, Eulberg D, Klussmann S, Anders HJ
    Diabetologia, 2009-08-26;52(11):2445-54.
    Species: Mouse
    Sample Types: Plasma
  77. Cell-nonautonomous function of Id1 in the hematopoietic progenitor cell niche.
    Authors: Suh HC, Ji M, Gooya J, Lee M, Klarmann KD, Keller JR
    Blood, 2009-05-28;114(6):1186-95.
    Species: Mouse
    Sample Types: Serum
  78. Synergy between CD26/DPP-IV inhibition and G-CSF improves cardiac function after acute myocardial infarction.
    Authors: Zaruba MM, Theiss HD, Vallaster M, Mehl U, Brunner S, David R, Fischer R, Krieg L, Hirsch E, Huber B, Nathan P, Israel L, Imhof A, Herbach N, Assmann G, Wanke R, Mueller-Hoecker J, Steinbeck G, Franz WM
    Cell Stem Cell, 2009-04-03;4(4):313-23.
    Species: Mouse
    Sample Types: Tissue Homogenates
  79. Endothelial nitric oxide synthase promotes bone marrow stromal cell migration to the ischemic myocardium via upregulation of stromal cell-derived factor-1alpha.
    Authors: Li N, Lu X, Zhao X, Xiang FL, Xenocostas A, Karmazyn M, Feng Q
    Stem Cells, 2009-04-01;27(4):961-70.
    Species: Mouse
    Sample Types: Tissue Homogenates
  80. The angiogenic response is dictated by beta3 integrin on bone marrow-derived cells.
    Authors: Feng W, McCabe NP, Mahabeleshwar GH, Somanath PR, Phillips DR, Byzova TV
    J. Cell Biol., 2008-12-15;183(6):1145-57.
    Species: Mouse
    Sample Types: Plasma
  81. Rosuvastatin reduces atherosclerotic lesions and promotes progenitor cell mobilisation and recruitment in apolipoprotein E knockout mice.
    Authors: Schroeter MR, Humboldt T, Schafer K, Konstantinides S
    Atherosclerosis, 2008-11-25;205(1):63-73.
    Species: Mouse
    Sample Types: Plasma
  82. Rapid chemotherapy-induced acute endothelial progenitor cell mobilization: implications for antiangiogenic drugs as chemosensitizing agents.
    Authors: Shaked Y, Henke E, Roodhart JM, Mancuso P, Langenberg MH, Colleoni M, Daenen LG, Man S, Xu P, Emmenegger U, Tang T, Zhu Z, Witte L, Strieter RM, Bertolini F, Voest EE, Benezra R, Kerbel RS
    Cancer Cell, 2008-09-09;14(3):263-73.
    Species: Mouse
    Sample Types: Plasma
  83. Antifibrotic action of pirfenidone and prednisolone: different effects on pulmonary cytokines and growth factors in bleomycin-induced murine pulmonary fibrosis.
    Authors: Oku H, Shimizu T, Kawabata T, Nagira M, Hikita I, Ueyama A, Matsushima S, Torii M, Arimura A
    Eur. J. Pharmacol., 2008-06-16;590(1):400-8.
    Species: Mouse
    Sample Types: Tissue Homogenates
  84. Heme oxygenase-1 promotes neovascularization in ischemic heart by coinduction of VEGF and SDF-1.
    Authors: Lin HH, Chen YH, Chang PF, Lee YT, Yet SF, Chau LY
    J. Mol. Cell. Cardiol., 2008-05-02;45(1):44-55.
    Species: Mouse
    Sample Types: Tissue Homogenates
  85. Altered SDF-1/CXCR4 axis in patients with primary myelofibrosis and in the Gata1 low mouse model of the disease.
    Authors: Migliaccio AR, Martelli F, Verrucci M, Migliaccio G, Vannucchi AM, Ni H, Xu M, Jiang Y, Nakamoto B, Papayannopoulou T, Hoffman R
    Exp. Hematol., 2008-02-01;36(2):158-71.
    Species: Mouse
    Sample Types: Plasma
  86. Multiple circulating proangiogenic factors induced by sunitinib malate are tumor-independent and correlate with antitumor efficacy.
    Authors: Ebos JM, Lee CR, Christensen JG, Mutsaers AJ, Kerbel RS
    Proc. Natl. Acad. Sci. U.S.A., 2007-10-17;104(43):17069-74.
    Species: Mouse
    Sample Types: Plasma
  87. Pulmonary stromal-derived factor-1 expression and effect on neutrophil recruitment during acute lung injury.
    Authors: Petty JM, Sueblinvong V, Lenox CC, Jones CC, Cosgrove GP, Cool CD, Rai PR, Brown KK, Weiss DJ, Poynter ME, Suratt BT
    J. Immunol., 2007-06-15;178(12):8148-57.
    Species: Mouse
    Sample Types: BALF
  88. Hypoxia-induced mitogenic factor has proangiogenic and proinflammatory effects in the lung via VEGF and VEGF receptor-2.
    Authors: Yamaji-Kegan K, Su Q, Angelini DJ, Champion HC, Johns RA
    Am. J. Physiol. Lung Cell Mol. Physiol., 2006-08-04;291(6):L1159-68.
    Species: Mouse, Rat
    Sample Types: Cell Culture Supernates
  89. Enalapril increases ischemia-induced endothelial progenitor cell mobilization through manipulation of the CD26 system.
    Authors: Wang CH, Verma S, Hsieh IC, Chen YJ, Kuo LT, Yang NI, Wang SY, Wu MY, Hsu CM, Cheng CW, Cherng WJ
    J. Mol. Cell. Cardiol., 2006-05-05;41(1):34-43.
    Species: Mouse
    Sample Types: Plasma
  90. Chemokine receptor expression in EBV-associated lymphoproliferation in hu/SCID mice: implications for CXCL12/CXCR4 axis in lymphoma generation.
    Authors: Piovan E, Tosello V, Indraccolo S, Cabrelle A, Baesso I, Trentin L, Zamarchi R, Tamamura H, Fujii N, Semenzato G, Chieco-Bianchi L, Amadori A
    Blood, 2004-09-28;105(3):931-9.
    Species: Mouse
    Sample Types: Peritoneal Lavage Fluid
  91. Recruitment of stem and progenitor cells from the bone marrow niche requires MMP-9 mediated release of kit-ligand.
    Authors: Heissig B, Hattori K, Dias S, Friedrich M, Ferris B, Hackett NR, Crystal RG, Besmer P, Lyden D, Moore MA, Werb Z, Rafii S
    Cell, 2002-05-31;109(5):625-37.
    Species: Mouse
    Sample Types: Plasma

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Mouse CXCL12/SDF-1 alpha Quantikine ELISA Kit
By Anonymous on 09/24/2023
Sample Tested: Mouse lymphocytes

We isolated mouse tumor tissues and collected serum samples to explore changes in the immune microenvironment of the TME and changes in immune-related indicators in the blood system.

Mouse CXCL12/SDF-1 alpha Quantikine ELISA Kit MCX120
Mouse CXCL12/SDF-1 alpha Quantikine ELISA Kit
By Ricardo Malvicini on 06/28/2022
Sample Tested: Cell culture supernatant

Cell culture supernatant from RAW2647 cells stimulated with LPS and LPS+MSC-EV

Mouse CXCL12/SDF-1 alpha Quantikine ELISA Kit MCX120
Mouse CXCL12/SDF-1 alpha Quantikine ELISA Kit
By Anonymous on 08/31/2021
Sample Tested: mouse cell line conditioned media
Mouse CXCL12/SDF-1 alpha Quantikine ELISA Kit
By Anonymous on 03/06/2021
Sample Tested: Human cell conditioned medium
Mouse CXCL12/SDF-1 alpha Quantikine ELISA Kit MCX120
Mouse CXCL12/SDF-1 alpha Quantikine ELISA Kit
By jayeeta giri on 07/08/2019
Sample Tested: Cell Lysates
Mouse CXCL12/SDF-1 alpha Quantikine ELISA Kit
By Anonymous on 03/08/2019
Sample Tested: mouse serum
Mouse CXCL12/SDF-1 alpha Quantikine ELISA Kit MCX120
Mouse CXCL12/SDF-1 alpha Quantikine ELISA Kit
By Anonymous on 04/30/2018
Sample Tested: 4T1.2 mouse mammary carcinoma cells
Mouse CXCL12/SDF-1 alpha Quantikine ELISA Kit
By Anonymous on 04/17/2018
Sample Tested: Pancreatic cancer cells
Mouse CXCL12/SDF-1 alpha Quantikine ELISA Kit
By Anonymous on 08/08/2016
Sample Tested: Bone marrow supernatant,Plasma

We have used this kit to check SDF gradient in mouse plasma and Bone marrow supernatant. Our results indicated that SDF gradient was shifted in favor of Bone marrow after treatment.

Mouse CXCL12/SDF-1 alpha Quantikine ELISA Kit
By Anonymous on 08/05/2016
Sample Tested: Mouse bone marrow supernatant,Mouse plasma

Very good precision and results are reliable. Published some data already and going to publish more.

Mouse CXCL12/SDF-1 alpha Quantikine ELISA Kit MCX120
Mouse CXCL12/SDF-1 alpha Quantikine ELISA Kit
By Adam Guess on 07/12/2016
Sample Tested: Bone marrow cells
Mouse CXCL12/SDF-1 alpha Quantikine ELISA Kit MCX120