Mouse CXCL13/BLC/BCA-1 Antibody

Catalog #: MAB4701
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4701-SP
MAB4701-500
MAB4701-100

Chemotaxis Induced by CXCL13/BLC/BCA‑1 and Neutralization by Mouse CXCL13/BLC/BCA‑1 Antibody.

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Mouse CXCL13/BLC/BCA-1 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CXCL13/BLC/BCA-1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant cotton rat CXCL10, recombinant human CXCL1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12/SDF-1 alpha, 12/SDF-1 beta, 13, 16, recombinant mouse CXCL1, 2, 4, 6, 7, 9, 10, 11, 12/SDF-1 alpha, 15, 16, recombinant porcine CXCL8, recombinant rat CXCL1, 2, 3/CINC-2 alpha, rr3/CINC-2 beta, or 5 is observed.

Source

Monoclonal Rat IgG_2A Clone # 143608

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant mouse CXCL13/BLC/BCA-1
Ile22-Ala109
Accession # O55038

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse CXCL13/BLC/BCA‑1 (Catalog # 470-BC)

Neutralization

Measured by its ability to neutralize CXCL13/BLC/BCA‑1-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR5. The Neutralization Dose (ND₅₀) is typically 12-30 µg/mL in the presence of 4 µg/mL Recombinant Mouse CXCL13/BLC/BCA‑1.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Chemotaxis Induced by CXCL13/BLC/BCA‑1 and Neutralization by Mouse CXCL13/BLC/BCA‑1 Antibody. Recombinant Mouse CXCL13/BLC/BCA-1 (Catalog # 470-BC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL13/BLC/BCA-1 (4 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CXCL13/BLC/BCA-1 Monoclonal Antibody (Catalog # MAB4701). The ND₅₀ is typically 12-30 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL13/BLC/BCA-1

CXCL13, also known as B-lymphocyte chemoattractant (BLC), is a CXC chemokine that is constitutively expressed in secondary lymphoid organs. Mouse BCA-1 cDNA encodes a precursor protein of 109 amino acid residues with a putative leader sequence of 21 residues. Mature mouse BCA-1 shares 64% amino acid sequence similarity with the human protein and 23‑34% amino acid sequence identity with other known CXC chemokines. Recombinant or chemically synthesized BCA-1 is a potent chemoattractant for B lymphocytes but not T lymphocytes, monocytes or neutrophils. BLR1, a G protein-coupled receptor originally isolated from Burkitt’s lymphoma cells, has now been shown to be the specific receptor for BCA-1. Among cells of the hematopoietic lineages, the expression of BLR1, now designated CXCR5, is restricted to B lymphocytes and a subpopulation of T helper memory cells. Mice lacking BLR1 have been shown to lack inguinal lymph nodes. These mice were also found to have impaired development of Peyer’s patches and defective formation of primary follicles and germinal centers in the spleen as a result of the inability of B lymphocytes to migrate into B cell areas.

References

Gunn, M.D. et al. (1998) Nature, 391:799.
Legler, D.F. et al. (1998) J. Exp. Med. 187:655.
Forster, R. et al. (1996) Cell 87:1037.

Entrez Gene IDs

10563 (Human); 55985 (Mouse)

Alternate Names

ANGIE; ANGIE2; B cell-attracting chemokine 1; B lymphocyte chemoattractant; BCA1; BCA-1; BCA1B-cell chemoattractant; BCA-1CXC chemokine BLC; B-cell-homing chemokine (ligand for Burkitt's lymphoma receptor-1); BLC; BLCSmall-inducible cytokine B13; BLR1L; B-lymphocyte chemoattractant; chemokine (C-X-C motif) ligand 13 (B-cell chemoattractant); chemokine (C-X-C motif) ligand 13; C-X-C motif chemokine 13; CXCL13; SCYB13; SCYB13B-cell-attracting chemokine 1; small inducible cytokine B subfamily (Cys-X-Cys motif), member 13 (B-cellchemoattractant)

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Citations for Mouse CXCL13/BLC/BCA-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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CXCR5(+) T helper cells mediate protective immunity against tuberculosis.
Authors: Slight S, Rangel-Moreno J, Gopal R, Lin Y, Fallert Junecko B, Mehra S, Selman M, Becerril-Villanueva E, Baquera-Heredia J, Pavon L, Kaushal D, Reinhart T, Randall T, Khader S
J Clin Invest, 2013-01-02;123(2):712-26.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P
Salmonella disrupts lymph node architecture by TLR4-mediated suppression of homeostatic chemokines.
Authors: St John AL, Abraham SN
Nat. Med., 2009-10-25;15(11):1259-65.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization