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Mouse CXCL13/BLC/BCA-1 Biotinylated Antibody

Catalog #: BAF470
8 Citations Datasheet / COA / SDS
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BAF470
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Citations (8)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CXCL13/BLC/BCA-1 Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CXCL13 in ELISAs and Western blots. In sandwich immunoassays, less than 0.05% cross-reactivity with recombinant human CXCL13, recombinant mouse (rm) I-TAC, rmMIG, and rmSDF-1 alpha is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse CXCL13/BLC (R&D Systems, Catalog # 470-BC)
Ile22-Ala109
Accession # Q3U1E8
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse CXCL13/BLC/BCA‑1 (Catalog # 470-BC)

Mouse CXCL13/BLC/BCA-1 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Mouse CXCL13/BLC/BCA‑1 Antibody (Catalog # MAB470R)

Capture Reagent: Mouse CXCL13/BLC/BCA‑1 Antibody (Catalog # MAB470)

Standard: Recombinant Mouse CXCL13/BLC/BCA-1 Protein (Catalog # 470-BC)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL13/BLC/BCA-1

CXCL13, also known as B-lymphocyte chemoattractant (BLC), is a CXC chemokine that is constitutively expressed in secondary lymphoid organs. Mouse BCA-1 cDNA encodes a precursor protein of 109 amino acid residues with a putative leader sequence of 21 residues. Mature mouse BCA-1 shares 64% amino acid sequence similarity with the human protein and 23 - 34% amino acid sequence identity with other known CXC chemokines. Recombinant or chemically synthesized BCA-1 is a potent chemoattractant for B lymphocytes but not T lymphocytes, monocytes or neutrophils. BLR1, a G protein-coupled receptor originally isolated from Burkitt’s lymphoma cells, has now been shown to be the specific receptor for BCA-1. Among cells of the hematopoietic lineages, the expression of BLR1, now designated CXCR5, is restricted to B lymphocytes and a subpopulation of T helper memory cells. Mice lacking BLR1 have been shown to lack inguinal lymph nodes. These mice were also found to have impaired development of Peyer’s patches and defective formation of primary follicles and germinal centers in the spleen as a result of the inability of B lymphocytes to migrate into B cell areas.

References
  1. Gunn, M.D. et al. (1998) Nature, 391:799.
  2. Legler, D.F. et al. (1998) J. Exp. Med. 187:655.
  3. Forster, R. et al. (1996) Cell 87:1037.
Entrez Gene IDs
10563 (Human); 55985 (Mouse)
Alternate Names
ANGIE; ANGIE2; B cell-attracting chemokine 1; B lymphocyte chemoattractant; BCA1; BCA-1; BCA1B-cell chemoattractant; BCA-1CXC chemokine BLC; B-cell-homing chemokine (ligand for Burkitt's lymphoma receptor-1); BLC; BLCSmall-inducible cytokine B13; BLR1L; B-lymphocyte chemoattractant; chemokine (C-X-C motif) ligand 13 (B-cell chemoattractant); chemokine (C-X-C motif) ligand 13; C-X-C motif chemokine 13; CXCL13; SCYB13; SCYB13B-cell-attracting chemokine 1; small inducible cytokine B subfamily (Cys-X-Cys motif), member 13 (B-cellchemoattractant)

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Citations for Mouse CXCL13/BLC/BCA-1 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Intestinal fibroblastic reticular cell niches control innate lymphoid cell homeostasis and function
    Authors: HW Cheng, U Mörbe, M Lütge, C Engetschwi, L Onder, M Novkovic, C Gil-Cruz, C Perez-Shib, T Hehlgans, E Scandella, B Ludewig
    Nature Communications, 2022-04-19;13(1):2027.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. CXCL13 Is an Indicator of Germinal Center Activity and Alloantibody Formation Following Transplantation
    Authors: Emma S. Crichton, Shan Zeng, G. Michael La Muraglia, I. Raul Badell
    Transplantation Direct
  3. Essential Role of Canonical NF-kappa B Activity in the Development of Stromal Cell Subsets in Secondary Lymphoid Organs
    Authors: Dana Bogdanova, Arata Takeuchi, Madoka Ozawa, Yasuhiro Kanda, M. Azizur Rahman, Burkhard Ludewig et al.
    The Journal of Immunology
  4. A Distinct Subset of Fibroblastic Stromal Cells Constitutes the Cortex-Medulla Boundary Subcompartment of the Lymph Node
    Authors: Arata Takeuchi, Madoka Ozawa, Yasuhiro Kanda, Mina Kozai, Izumi Ohigashi, Yoichi Kurosawa et al.
    Frontiers in Immunology
  5. Peyer's patch inducer cells play a leading role in the formation of B and T cell zone architecture.
    Authors: Nakagawa R, Togawa A, Nagasawa T, Nishikawa S
    J Immunol, 2013-03-04;190(7):3309-18.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. Colonic patch and colonic SILT development are independent and differentially regulated events.
    Authors: Baptista A, Olivier B, Goverse G, Greuter M, Knippenberg M, Kusser K, Domingues R, Veiga-Fernandes H, Luster A, Lugering A, Randall T, Cupedo T, Mebius R
    Mucosal Immunol, 2012-09-19;6(3):511-21.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. Response patterns of cytokines/chemokines in two murine strains after irradiation.
    Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
    Cytokine, 2012-01-25;58(2):169-77.
    Species: Mouse
    Sample Types: Plasma
    Applications: Luminex Development
  8. Organizer-like reticular stromal cell layer common to adult secondary lymphoid organs.
    Authors: Katakai T, Suto H, Sugai M, Gonda H, Togawa A, Suematsu S, Ebisuno Y, Katagiri K, Kinashi T, Shimizu A
    J. Immunol., 2008-11-01;181(9):6189-200.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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