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Mouse CXCR3 Antibody

Catalog #: MAB1685
14 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1685-100
MAB1685-SP
Detection of CXCR3 in Mouse Splenocytes by Flow Cytometry.
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Product Details
Citations (14)
FAQs
Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CXCR3 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CXCR3.
Source
Monoclonal Rat IgG2A Clone # 220803
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
CHO Chinese hamster ovary cell line transfected with mouse CXCR3
Met1-Leu367
Accession # AAC40163
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CXCR3 antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of CXCR3 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD3 APC-conjugated Monoclonal Antibody (Catalog # FAB4841A) and either (A) Rat Anti-Mouse CXCR3 Monoclonal Antibody (Catalog # MAB1685) or (B) Rat IgG2AIsotype Control (Catalog # MAB006) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of Mouse CXCR3 by Flow Cytometry View Larger

Detection of Mouse CXCR3 by Flow Cytometry Priming of pik3cg−/− CD4+ T cells is reduced during EAE.(A) Frequencies of CD4+ cells in draining LN that are CD69+ on day 9 post-immunisation for EAE as determined by flow cytometry. A representative histogram overlay gating on CD4+ cells is shown (filled = isotype control on WT, solid line = anti-CD69 on WT, dotted line = anti-CD69 on pik3cg−/−) (B) In vivo proliferation of CD4+ cells, measured by BrdU incorporation, is reduced in pik3cg−/− mice at day 9 post-immunization for EAE. (n = 6 mice per group). Representative dot plots gating on CD4+ cells are shown. (C) Expression of chemokine receptors by CD4+ T cells indicative of T cell activation was determined by flow cytometry. Representative histogram overlays showing expression of CCR7, CCR6 and CXCR3 are shown (filled = isotype control, solid line = WT, dotted line = pik3cg−/−). (D) Expression of CD62L, CD49d and PSGL-1 by CD4+ cells from spleen of day 9 immunised mice (n = 3 mice per group). All data shown are mean ± s.e.m. (*, p<0.05). Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0045095), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCR3

CXCR3 is a G protein coupled chemokine receptor that binds the alpha chemokines MIG (CXCL9), IP-10 (CXCL10) and I-TAC (CXCL11). CXCR3 is expressed on activated T cells, B cells and NK cells.

Entrez Gene IDs
2833 (Human); 12766 (Mouse); 84475 (Rat)
Alternate Names
CD183 antigen; CD183; chemokine (C-X-C motif) receptor 3; chemokine (C-X-C) receptor 3; CKR-L2IP-10 receptor; CMKAR3; C-X-C chemokine receptor type 3; CXCR3; CXC-R3; CXCR-3; G protein-coupled receptor 9CD182; GPR9; GPR9Mig-R; Interferon-inducible protein 10 receptor; IP10 receptor; IP10-R; Mig receptor; MigR

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Citations for Mouse CXCR3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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  1. PI3K gamma drives priming and survival of autoreactive CD4(+) T cells during experimental autoimmune encephalomyelitis
    Authors: Iain Comerford, Wendel Litchfield, Ervin Kara, Shaun R. McColl
    PLoS ONE
  2. Rapid Internalization and Nuclear Translocation of CCL5 and CXCL4 in Endothelial Cells
    Authors: A Dickhout, DM Kaczor, ACA Heinzmann, SLN Brouns, JWM Heemskerk, MAMJ van Zandvo, RR Koenen
    International Journal of Molecular Sciences, 2021-07-08;22(14):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  3. Quantitative single-cell interactomes in normal and virus-infected mouse lungs
    Authors: MP Cain, BJ Hernandez, J Chen
    Dis Model Mech, 2020-06-26;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Classical Type 1 Dendritic Cells Dominate Priming of Th1 Responses to Herpes Simplex Virus Type 1 Skin Infection
    Authors: CM Harpur, Y Kato, ST Dewi, S Stankovic, DN Johnson, S Bedoui, PG Whitney, MH Lahoud, I Caminschi, WR Heath, AG Brooks, T Gebhardt
    J. Immunol., 2018-12-31;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. CD4 T cell epitope specificity and cytokine potential are preserved as cells transition from the lung vasculature to lung tissue following influenza infection
    Authors: A DiPiazza, N Laniewski, A Rattan, DJ Topham, J Miller, AJ Sant
    J. Virol., 2018-06-13;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. The histone demethylase UTX regulates the lineage-specific epigenetic program of invariant natural killer T cells
    Nat. Immunol, 2016-12-19;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. IL-10+ Innate-like B Cells Are Part of the Skin Immune System and Require ?4?1 Integrin To Migrate between the Peritoneum and Inflamed Skin
    Authors: SA Geherin, D Gómez, RA Glabman, G Ruthel, A Hamann, GF Debes
    J. Immunol, 2016-02-05;196(6):2514-25.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. NKG2D ligand overexpression in lupus nephritis correlates with increased NK cell activity and differentiation in kidneys but not in the periphery.
    Authors: Spada R, Rojas J, Perez-Yague S, Mulens V, Cannata-Ortiz P, Bragado R, Barber D
    J Leukoc Biol, 2015-01-12;97(3):583-98.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. IL-21 promotes late activator APC-mediated T follicular helper cell differentiation in experimental pulmonary virus infection.
    Authors: Yoo J, Braciale T
    PLoS ONE, 2014-09-24;9(9):e105872.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Flow Cytometry
  10. Early differentiated CD138(high) MHCII+ IgG+ plasma cells express CXCR3 and localize into inflamed kidneys of lupus mice.
    Authors: Lacotte S, Decossas M, Le Coz C, Brun S, Muller S, Dumortier H
    PLoS ONE, 2013-03-08;8(3):e58140.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  11. T cells dampen innate immune responses through inhibition of NLRP1 and NLRP3 inflammasomes.
    Authors: Guarda G, Dostert C, Staehli F, Cabalzar K, Castillo R, Tardivel A, Schneider P, Tschopp J
    Nature, 2009-06-03;460(7252):269-73.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. L-selectin-negative CCR7- effector and memory CD8+ T cells enter reactive lymph nodes and kill dendritic cells.
    Authors: Guarda G, Hons M, Soriano SF, Huang AY, Polley R, Martin-Fontecha A, Stein JV, Germain RN, Lanzavecchia A, Sallusto F
    Nat. Immunol., 2007-05-27;8(7):743-52.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. Temporal dissection of T-bet functions.
    Authors: Matsuda JL, George TC, Hagman J, Gapin L
    J. Immunol., 2007-03-15;178(6):3457-65.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. CXCR3-dependent recruitment of antigen-specific T lymphocytes to the liver during murine cytomegalovirus infection.
    Authors: Hokeness KL, Deweerd ES, Munks MW, Lewis CA, Gladue RP, Salazar-Mather TP
    J. Virol., 2006-11-15;81(3):1241-50.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Mouse CXCR3 Antibody
By JEEHO LEE on 08/04/2016
Application: Flow Sample Tested: T cells Species: Mouse

murine splenic CD4 T cells were stained.

Flow Cytometry Mouse CXCR3 Antibody MAB1685